Quantitative paper-based detection of male fertility biomarkers

Male infertility is a reproductive disorder culminating from hormonal imbalances which may be caused by stress and use of health supplements. Invasive diagnostic tests and privacy issues have relatively deterred men from seeking infertility treatment. A rapid and reliable test using non-invasive sam...

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Main Authors:	Abdul Rahman, Suzanah, Mohd Zahri, Syazana, Abdul Majid, Zafri Azran, Ahmad, Nur Amalina, Abdul Samat, Nadia Hanis, Abdullah, Afif Raihan
Format:	Article
Language:	English English
Published:	Pharmascope Publications 2018
Subjects:	Q Science (General) TP248.13 Biotechnology TR510 Color photography
Online Access:	http://irep.iium.edu.my/69878/ http://irep.iium.edu.my/69878/ http://irep.iium.edu.my/69878/ http://irep.iium.edu.my/69878/1/IJRPS%202018.pdf http://irep.iium.edu.my/69878/7/69878_Quantitative%20paper-based%20detection_scopus.pdf

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recordtype	eprints
spelling	iium-698782019-07-12T08:43:21Z http://irep.iium.edu.my/69878/ Quantitative paper-based detection of male fertility biomarkers Abdul Rahman, Suzanah Mohd Zahri, Syazana Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, Afif Raihan Q Science (General) TP248.13 Biotechnology TR510 Color photography Male infertility is a reproductive disorder culminating from hormonal imbalances which may be caused by stress and use of health supplements. Invasive diagnostic tests and privacy issues have relatively deterred men from seeking infertility treatment. A rapid and reliable test using non-invasive samples is sought to observe the reproductive effects of subchronic thymoquinone administration and prolonged artificial light exposure to rats. Detection of testosterone as the male fertility biomarker from non-invasive samples was employed using adult male Sprague-Dawley rats treated with corticosterone (10 mg/kg) for 10 successive days, followed by a single dose of cisplatin (10 mg/kg), prior to the day of sacrifice. All interventions were given within a 56 days period and sampling of blood, saliva and urine were performed at day 0 and day 56. Testosterone and corticosterone levels were determined using enzyme-linked immunosorbent assay (ELISA). Sperm analysis parameters subsequent to testes harvest at day 56 were measured and followed by a histological assessment. Paper-based lateral flow assay (PLFA) strip was developed based on the colour change from the antigen-antibody reaction on paper reflecting testosterone levels from urine samples. The colour change was recorded using a smartphone camera by an application that captures the RGB colour value. This study demonstrated that 30mg/kg subchronic thymoquinone supplementation can reduce testosterone levels thus possibly affecting fertility. Meanwhile, the 24-hour light exposure showed no significant effects compared to controls. The testosterone level assessment using the PLFA produced comparable data with ELISA results. This sensor holds potential to increase patient compliance with sampling by using non-invasive samples to test infertility in men. Pharmascope Publications 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69878/1/IJRPS%202018.pdf application/pdf en http://irep.iium.edu.my/69878/7/69878_Quantitative%20paper-based%20detection_scopus.pdf Abdul Rahman, Suzanah and Mohd Zahri, Syazana and Abdul Majid, Zafri Azran and Ahmad, Nur Amalina and Abdul Samat, Nadia Hanis and Abdullah, Afif Raihan (2018) Quantitative paper-based detection of male fertility biomarkers. International Journal of Research in Pharmaceutical Sciences, 9 (Special Issue 2). pp. 17-26. E-ISSN 0975-7538 https://www.pharmascope.org/~pharmascope/index.php/ijrps/article/view/1732/955 10.26452/ijrps.v9iSPL2.1732
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) TP248.13 Biotechnology TR510 Color photography
spellingShingle	Q Science (General) TP248.13 Biotechnology TR510 Color photography Abdul Rahman, Suzanah Mohd Zahri, Syazana Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, Afif Raihan Quantitative paper-based detection of male fertility biomarkers
description	Male infertility is a reproductive disorder culminating from hormonal imbalances which may be caused by stress and use of health supplements. Invasive diagnostic tests and privacy issues have relatively deterred men from seeking infertility treatment. A rapid and reliable test using non-invasive samples is sought to observe the reproductive effects of subchronic thymoquinone administration and prolonged artificial light exposure to rats. Detection of testosterone as the male fertility biomarker from non-invasive samples was employed using adult male Sprague-Dawley rats treated with corticosterone (10 mg/kg) for 10 successive days, followed by a single dose of cisplatin (10 mg/kg), prior to the day of sacrifice. All interventions were given within a 56 days period and sampling of blood, saliva and urine were performed at day 0 and day 56. Testosterone and corticosterone levels were determined using enzyme-linked immunosorbent assay (ELISA). Sperm analysis parameters subsequent to testes harvest at day 56 were measured and followed by a histological assessment. Paper-based lateral flow assay (PLFA) strip was developed based on the colour change from the antigen-antibody reaction on paper reflecting testosterone levels from urine samples. The colour change was recorded using a smartphone camera by an application that captures the RGB colour value. This study demonstrated that 30mg/kg subchronic thymoquinone supplementation can reduce testosterone levels thus possibly affecting fertility. Meanwhile, the 24-hour light exposure showed no significant effects compared to controls. The testosterone level assessment using the PLFA produced comparable data with ELISA results. This sensor holds potential to increase patient compliance with sampling by using non-invasive samples to test infertility in men.
format	Article
author	Abdul Rahman, Suzanah Mohd Zahri, Syazana Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, Afif Raihan
author_facet	Abdul Rahman, Suzanah Mohd Zahri, Syazana Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, Afif Raihan
author_sort	Abdul Rahman, Suzanah
title	Quantitative paper-based detection of male fertility biomarkers
title_short	Quantitative paper-based detection of male fertility biomarkers
title_full	Quantitative paper-based detection of male fertility biomarkers
title_fullStr	Quantitative paper-based detection of male fertility biomarkers
title_full_unstemmed	Quantitative paper-based detection of male fertility biomarkers
title_sort	quantitative paper-based detection of male fertility biomarkers
publisher	Pharmascope Publications
publishDate	2018
url	http://irep.iium.edu.my/69878/ http://irep.iium.edu.my/69878/ http://irep.iium.edu.my/69878/ http://irep.iium.edu.my/69878/1/IJRPS%202018.pdf http://irep.iium.edu.my/69878/7/69878_Quantitative%20paper-based%20detection_scopus.pdf
first_indexed	2023-09-18T21:39:12Z
last_indexed	2023-09-18T21:39:12Z
_version_	1777413025754513408

Quantitative paper-based detection of male fertility biomarkers

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