Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)

The objective of this study was to observe the effect of 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-trichloro picolinic acid (Picloram) on callus induction of Mas Cotek (Ficus deltoidea var bilobata) explant. Murashige and Skoog (MS) nutrient media supplemented with 6-Benzylaminopurine (BAP) and...

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Main Authors:	Ismail, Azrina, Shahidan, Norshazila, Othman, Rashidi, Mat, Nashriyah
Format:	Article
Language:	English
Published:	Science Publishing Corporation Inc 2018
Subjects:	S Agriculture (General) SB Plant culture TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/69830/ http://irep.iium.edu.my/69830/ http://irep.iium.edu.my/69830/ http://irep.iium.edu.my/69830/1/69830_Callus%20Induction%20from%20Leaf%20Explants%20of%20Mas%20Cotek.pdf

id	iium-69830
recordtype	eprints
spelling	iium-698302019-07-12T08:52:26Z http://irep.iium.edu.my/69830/ Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram) Ismail, Azrina Shahidan, Norshazila Othman, Rashidi Mat, Nashriyah S Agriculture (General) SB Plant culture TP248.13 Biotechnology The objective of this study was to observe the effect of 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-trichloro picolinic acid (Picloram) on callus induction of Mas Cotek (Ficus deltoidea var bilobata) explant. Murashige and Skoog (MS) nutrient media supplemented with 6-Benzylaminopurine (BAP) and 4-amino-3,5,6-trichloro picolinic acid (Picloram) each at different concentrations of 0 mg/l, 0.5 mg/l , 1.5 mg/l and 3.0 mg/l, respectively. The control and other six treatments gave negative responses to the MS media supplemented with various concentrations of BAP and Picloram. Most of the callus began to form at day 16 up to day 30 with treatment. Hard and yellowish callus with moderate growth rate was obtained from explants cultured on MS medium supplemented with 0.5 mg/ l BAP and 1.5 mg/l Picloram, wherein the callus formation was recorded at 47 %. Hard and greenish callus was obtained from medium treated with 0.5 mg/l BAP and 0.5 mg/l Picloram after 30 days of treatment with callus formation of 26.67 %. Medium supplemented with 0.5 mg/l of BAP hormone and 1.5 mg/l of Picloram hormone displayed the best growth rate of callus and was the most suitable medium for callus induction. Science Publishing Corporation Inc 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69830/1/69830_Callus%20Induction%20from%20Leaf%20Explants%20of%20Mas%20Cotek.pdf Ismail, Azrina and Shahidan, Norshazila and Othman, Rashidi and Mat, Nashriyah (2018) Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram). International Journal of Engineering and Technology, 7 (4.43). pp. 164-167. ISSN 2227-524X https://www.sciencepubco.com/index.php/ijet/article/view/25841 10.14419/ijet.v7i4.43.25841
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	S Agriculture (General) SB Plant culture TP248.13 Biotechnology
spellingShingle	S Agriculture (General) SB Plant culture TP248.13 Biotechnology Ismail, Azrina Shahidan, Norshazila Othman, Rashidi Mat, Nashriyah Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
description	The objective of this study was to observe the effect of 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-trichloro picolinic acid (Picloram) on callus induction of Mas Cotek (Ficus deltoidea var bilobata) explant. Murashige and Skoog (MS) nutrient media supplemented with 6-Benzylaminopurine (BAP) and 4-amino-3,5,6-trichloro picolinic acid (Picloram) each at different concentrations of 0 mg/l, 0.5 mg/l , 1.5 mg/l and 3.0 mg/l, respectively. The control and other six treatments gave negative responses to the MS media supplemented with various concentrations of BAP and Picloram. Most of the callus began to form at day 16 up to day 30 with treatment. Hard and yellowish callus with moderate growth rate was obtained from explants cultured on MS medium supplemented with 0.5 mg/ l BAP and 1.5 mg/l Picloram, wherein the callus formation was recorded at 47 %. Hard and greenish callus was obtained from medium treated with 0.5 mg/l BAP and 0.5 mg/l Picloram after 30 days of treatment with callus formation of 26.67 %. Medium supplemented with 0.5 mg/l of BAP hormone and 1.5 mg/l of Picloram hormone displayed the best growth rate of callus and was the most suitable medium for callus induction.
format	Article
author	Ismail, Azrina Shahidan, Norshazila Othman, Rashidi Mat, Nashriyah
author_facet	Ismail, Azrina Shahidan, Norshazila Othman, Rashidi Mat, Nashriyah
author_sort	Ismail, Azrina
title	Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_short	Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_full	Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_fullStr	Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_full_unstemmed	Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)
title_sort	callus induction from leaf explants of mas cotek (ficus deltoidea var. bilobata) treated with 6-benzylaminopurine (bap) and 4-amino-3, 5, 6-trichloropicolinic acid (picloram)
publisher	Science Publishing Corporation Inc
publishDate	2018
url	http://irep.iium.edu.my/69830/ http://irep.iium.edu.my/69830/ http://irep.iium.edu.my/69830/ http://irep.iium.edu.my/69830/1/69830_Callus%20Induction%20from%20Leaf%20Explants%20of%20Mas%20Cotek.pdf
first_indexed	2023-09-18T21:39:08Z
last_indexed	2023-09-18T21:39:08Z
_version_	1777413021352591360

Callus Induction from leaf explants of Mas Cotek (Ficus Deltoidea var. bilobata) treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)

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