Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data

Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of thi...

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Main Authors:	Waludin, Shaidatul Nadia, Mohamed, Ahmad Azfar, Mukai, Yukinori
Format:	Article
Language:	English English
Published:	Malaysian Society of Applied Biology 2018
Subjects:	QL Zoology SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/69802/ http://irep.iium.edu.my/69802/ http://irep.iium.edu.my/69802/1/69802_Genetic%20diversity%20of%20the%20orange-spotted.pdf http://irep.iium.edu.my/69802/7/69802_Genetic%20diversity%20of%20the%20orange-spotted%20grouper_scopus.pdf

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recordtype	eprints
spelling	iium-698022019-02-26T01:34:58Z http://irep.iium.edu.my/69802/ Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori QL Zoology SH Aquaculture. Fisheries. Angling Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of this study was to investigate the genetic diversity of wild orange-spotted grouper populations in Terengganu via partial cytochrome b (cyt-b) gene analysis. A total of 60 fish, which were reared from wild seed fish, were collected from farms in Besut and Setiu, Terengganu. Their DNA was extracted using the Qiagen Blood and Tissue kit. PCR amplification was conducted using cyt-b primers, and a sequence of 460 bp in length was obtained for each sample. Next, phylogenetic analysis was performed to study the relationships among the individuals. The haplotype and nucleotide diversities of the populations were investigated to measure genetic diversity. The haplotype diversity (Hd) of E. coioides was relatively low for both Besut (Hd = 0.4161) and Setiu (Hd = 0.8782) populations. The nucleotide diversity (π) for both Besut and Setiu populations was also low. Moreover, 15 haplotypes were identified among the 60 individuals, with 33 individuals sharing a single haplotype (Hap01). These findings similar with a previous study that reported that seed fish dependent can led to a decline in grouper numbers and in turn cause genetic deprivation in the population Malaysian Society of Applied Biology 2018-12-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69802/1/69802_Genetic%20diversity%20of%20the%20orange-spotted.pdf application/pdf en http://irep.iium.edu.my/69802/7/69802_Genetic%20diversity%20of%20the%20orange-spotted%20grouper_scopus.pdf Waludin, Shaidatul Nadia and Mohamed, Ahmad Azfar and Mukai, Yukinori (2018) Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data. Malaysia Applied Biology, 47 (6). pp. 61-68. http://www.mabjournal.com/images/47_6_December_2018/47_06_08.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QL Zoology SH Aquaculture. Fisheries. Angling
spellingShingle	QL Zoology SH Aquaculture. Fisheries. Angling Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
description	Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of this study was to investigate the genetic diversity of wild orange-spotted grouper populations in Terengganu via partial cytochrome b (cyt-b) gene analysis. A total of 60 fish, which were reared from wild seed fish, were collected from farms in Besut and Setiu, Terengganu. Their DNA was extracted using the Qiagen Blood and Tissue kit. PCR amplification was conducted using cyt-b primers, and a sequence of 460 bp in length was obtained for each sample. Next, phylogenetic analysis was performed to study the relationships among the individuals. The haplotype and nucleotide diversities of the populations were investigated to measure genetic diversity. The haplotype diversity (Hd) of E. coioides was relatively low for both Besut (Hd = 0.4161) and Setiu (Hd = 0.8782) populations. The nucleotide diversity (π) for both Besut and Setiu populations was also low. Moreover, 15 haplotypes were identified among the 60 individuals, with 33 individuals sharing a single haplotype (Hap01). These findings similar with a previous study that reported that seed fish dependent can led to a decline in grouper numbers and in turn cause genetic deprivation in the population
format	Article
author	Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori
author_facet	Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori
author_sort	Waludin, Shaidatul Nadia
title	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_short	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_full	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_fullStr	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_full_unstemmed	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_sort	genetic diversity of the orange-spotted grouper (epinephelus coioides) in terengganu malaysia based on mitochondrial cytochrome b sequence data
publisher	Malaysian Society of Applied Biology
publishDate	2018
url	http://irep.iium.edu.my/69802/ http://irep.iium.edu.my/69802/ http://irep.iium.edu.my/69802/1/69802_Genetic%20diversity%20of%20the%20orange-spotted.pdf http://irep.iium.edu.my/69802/7/69802_Genetic%20diversity%20of%20the%20orange-spotted%20grouper_scopus.pdf
first_indexed	2023-09-18T21:39:06Z
last_indexed	2023-09-18T21:39:06Z
_version_	1777413019374977024

Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data

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