Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang

Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation....

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Main Authors:	Azizan, Nur Hafizah, Md Rami, Siti Zakiah, Saedudin, RD Rohmat, Kasim, Shahreen
Format:	Article
Language:	English English English
Published:	Penerbit UTHM 2018
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/1/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading.pdf http://irep.iium.edu.my/69676/2/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_SCOPUS.pdf http://irep.iium.edu.my/69676/3/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_WOS.pdf

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spelling	iium-696762019-01-29T04:15:08Z http://irep.iium.edu.my/69676/ Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen Q Science (General) Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation. No profound studies have yet been done in the construction site at Tanah Rata. Hence, this research was carried out to find existing status of microbial community from a few selected spots. Enrichment culture technique by using MSM broth has been used to isolate the desired microorganisms. Isolation and characterization tests using phenotypic and genotypic approaches (based on genes encoding 16S rRNA) had led to the discovery of 18 isolates. The 16S rRNA was used due to its functional constant, universally distributed and moderately well discovered across broad phylogenetic distances. The successfully identified genera were Pseudomonas, Bacillus, Exiguobacterium, Stenotrophomonas, Acinetobacter, Serratia and Gamma Proteobacterium. Penerbit UTHM 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69676/1/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading.pdf application/pdf en http://irep.iium.edu.my/69676/2/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/69676/3/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_WOS.pdf Azizan, Nur Hafizah and Md Rami, Siti Zakiah and Saedudin, RD Rohmat and Kasim, Shahreen (2018) Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang. International Journal of Integrated Engineering, 10 (6). pp. 128-132. ISSN 2229-838X E-ISSN 2600-7916 http://penerbit.uthm.edu.my/ojs/index.php/ijie/article/view/2773/1771 https://doi.org/10.30880/ijie.2018.10.06.017
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang
description	Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation. No profound studies have yet been done in the construction site at Tanah Rata. Hence, this research was carried out to find existing status of microbial community from a few selected spots. Enrichment culture technique by using MSM broth has been used to isolate the desired microorganisms. Isolation and characterization tests using phenotypic and genotypic approaches (based on genes encoding 16S rRNA) had led to the discovery of 18 isolates. The 16S rRNA was used due to its functional constant, universally distributed and moderately well discovered across broad phylogenetic distances. The successfully identified genera were Pseudomonas, Bacillus, Exiguobacterium, Stenotrophomonas, Acinetobacter, Serratia and Gamma Proteobacterium.
format	Article
author	Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen
author_facet	Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen
author_sort	Azizan, Nur Hafizah
title	Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang
title_short	Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang
title_full	Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang
title_fullStr	Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang
title_full_unstemmed	Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang
title_sort	phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of pahang
publisher	Penerbit UTHM
publishDate	2018
url	http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/1/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading.pdf http://irep.iium.edu.my/69676/2/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_SCOPUS.pdf http://irep.iium.edu.my/69676/3/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_WOS.pdf
first_indexed	2023-09-18T21:38:54Z
last_indexed	2023-09-18T21:38:54Z
_version_	1777413007198912512

Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang

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