Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.

The aims of the study were to investigate the anti-cancer effects of 5Aza and TSA in two leukemic cell lines (CCRF-CEM and HL-60). Inhibition concentration of 5-Aza and TSA were measured using trypan blue exclusion assay. 5-Aza and TSA at IC50 were treated to both CCRF-CEM and HL-60 cell lines for 4...

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Main Authors:	Aziee, Sudin, Haiyuni, Mohd Yassim, Nagi Al-Jamal, Hamid Ali, Shafini, Mohamed Yusoff, Abdul Wahab, Ridhwan, Shamsuddin, Shaharum, Johan, Muhammad Farid
Format:	Article
Language:	English
Published:	Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia. 2018
Subjects:	RM147 Administration of Drugs and Other Therapeutic Agents
Online Access:	http://irep.iium.edu.my/69651/ http://irep.iium.edu.my/69651/ http://irep.iium.edu.my/69651/1/69651_Apoptotic%20Induction%20in%20CCRF-CEM.pdf

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spelling	iium-696512019-02-15T09:28:07Z http://irep.iium.edu.my/69651/ Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A. Aziee, Sudin Haiyuni, Mohd Yassim Nagi Al-Jamal, Hamid Ali Shafini, Mohamed Yusoff Abdul Wahab, Ridhwan Shamsuddin, Shaharum Johan, Muhammad Farid RM147 Administration of Drugs and Other Therapeutic Agents The aims of the study were to investigate the anti-cancer effects of 5Aza and TSA in two leukemic cell lines (CCRF-CEM and HL-60). Inhibition concentration of 5-Aza and TSA were measured using trypan blue exclusion assay. 5-Aza and TSA at IC50 were treated to both CCRF-CEM and HL-60 cell lines for 4-6 days. To confirm the inhibition effects of these agents, Annexin-V stained cells were analyzed using flow cytometry to evaluate the apoptotic induction. The IC50 values of CCRF-CEM were 2.01±0.1µM and 2.65±0.3µM for 5-Aza- and TSA-treated, respectively. Whereas, the IC50 values of HL-60 were 1.98±0.2µM and 2.35±0.2µM for 5-Aza- and TSA-treated, respectively. To further substantiate the findings, the time-dependent exposure of both drugs was studied. CCRF-CEM cells were reduced to 49.4%±5.0, 49.4%±2.5 and 41.5%±5.6 by 5-Aza; 56.5%±7.0, 45.3%±4.2 and 40.2%±4.2 by TSA treatment at first, third and sixth day. HL-60 cells were reduced to 72.0%±4.5, 51.0%±1.5 and 40.6%±2.6 by 5-Aza at first, third and sixth day. Meanwhile, HL-60 cells reduced to 55.6%±4.5, 45.2%±4.0 and 36.3%±2.9 by TSA at first, second and fourth day. Both cell lines were significantly inhibited (p<0.05) compared to the untreated. Furthermore, flow cytometry demonstrated that 5-Aza and TSA significantly increased the cells population positive for Annexin-V in CCRF-CEM and HL-60 cell lines. In CCRF-CEM, the total apoptotic rates were 51.7%±9.7 and 49.4%±6.0 for 5-Aza- and TSA-treated, while, in HL-60, the apoptotic rates were 51.0%±3.9 and 49.7%±9.6 for 5-Aza- and TSA-treated, in a dose- and time-dependent manner, respectively. Epigenetic modification drugs, 5-Aza and TSA have anti-leukemic effects and induce apoptosis at micro-molar concentrations in CCRF-CEM and HL-60 leukemic cell lines. These results may provide a new insight into the use of 5-Aza and TSA in inhibiting the growth of leukemic cells and useful strategy in developing an epigenetic therapy. Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia. 2018-08-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69651/1/69651_Apoptotic%20Induction%20in%20CCRF-CEM.pdf Aziee, Sudin and Haiyuni, Mohd Yassim and Nagi Al-Jamal, Hamid Ali and Shafini, Mohamed Yusoff and Abdul Wahab, Ridhwan and Shamsuddin, Shaharum and Johan, Muhammad Farid (2018) Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A. Journal of Biomedical and Clinical Sciences (JBCS), 3 (1). pp. 54-61. E-ISSN 2550-147X http://apps.amdi.usm.my/journal/index.php/jbcs/article/view/160
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM147 Administration of Drugs and Other Therapeutic Agents
spellingShingle	RM147 Administration of Drugs and Other Therapeutic Agents Aziee, Sudin Haiyuni, Mohd Yassim Nagi Al-Jamal, Hamid Ali Shafini, Mohamed Yusoff Abdul Wahab, Ridhwan Shamsuddin, Shaharum Johan, Muhammad Farid Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.
description	The aims of the study were to investigate the anti-cancer effects of 5Aza and TSA in two leukemic cell lines (CCRF-CEM and HL-60). Inhibition concentration of 5-Aza and TSA were measured using trypan blue exclusion assay. 5-Aza and TSA at IC50 were treated to both CCRF-CEM and HL-60 cell lines for 4-6 days. To confirm the inhibition effects of these agents, Annexin-V stained cells were analyzed using flow cytometry to evaluate the apoptotic induction. The IC50 values of CCRF-CEM were 2.01±0.1µM and 2.65±0.3µM for 5-Aza- and TSA-treated, respectively. Whereas, the IC50 values of HL-60 were 1.98±0.2µM and 2.35±0.2µM for 5-Aza- and TSA-treated, respectively. To further substantiate the findings, the time-dependent exposure of both drugs was studied. CCRF-CEM cells were reduced to 49.4%±5.0, 49.4%±2.5 and 41.5%±5.6 by 5-Aza; 56.5%±7.0, 45.3%±4.2 and 40.2%±4.2 by TSA treatment at first, third and sixth day. HL-60 cells were reduced to 72.0%±4.5, 51.0%±1.5 and 40.6%±2.6 by 5-Aza at first, third and sixth day. Meanwhile, HL-60 cells reduced to 55.6%±4.5, 45.2%±4.0 and 36.3%±2.9 by TSA at first, second and fourth day. Both cell lines were significantly inhibited (p<0.05) compared to the untreated. Furthermore, flow cytometry demonstrated that 5-Aza and TSA significantly increased the cells population positive for Annexin-V in CCRF-CEM and HL-60 cell lines. In CCRF-CEM, the total apoptotic rates were 51.7%±9.7 and 49.4%±6.0 for 5-Aza- and TSA-treated, while, in HL-60, the apoptotic rates were 51.0%±3.9 and 49.7%±9.6 for 5-Aza- and TSA-treated, in a dose- and time-dependent manner, respectively. Epigenetic modification drugs, 5-Aza and TSA have anti-leukemic effects and induce apoptosis at micro-molar concentrations in CCRF-CEM and HL-60 leukemic cell lines. These results may provide a new insight into the use of 5-Aza and TSA in inhibiting the growth of leukemic cells and useful strategy in developing an epigenetic therapy.
format	Article
author	Aziee, Sudin Haiyuni, Mohd Yassim Nagi Al-Jamal, Hamid Ali Shafini, Mohamed Yusoff Abdul Wahab, Ridhwan Shamsuddin, Shaharum Johan, Muhammad Farid
author_facet	Aziee, Sudin Haiyuni, Mohd Yassim Nagi Al-Jamal, Hamid Ali Shafini, Mohamed Yusoff Abdul Wahab, Ridhwan Shamsuddin, Shaharum Johan, Muhammad Farid
author_sort	Aziee, Sudin
title	Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.
title_short	Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.
title_full	Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.
title_fullStr	Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.
title_full_unstemmed	Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.
title_sort	apoptotic induction in ccrf-cem and hl-60 human leukemic cell lines by 5-azacitidine and trichostatin a.
publisher	Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia.
publishDate	2018
url	http://irep.iium.edu.my/69651/ http://irep.iium.edu.my/69651/ http://irep.iium.edu.my/69651/1/69651_Apoptotic%20Induction%20in%20CCRF-CEM.pdf
first_indexed	2023-09-18T21:38:52Z
last_indexed	2023-09-18T21:38:52Z
_version_	1777413004576423936

Apoptotic induction in CCRF-CEM and HL-60 human leukemic cell lines by 5-Azacitidine and trichostatin A.

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