Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines

Nine phenolic compounds were identified and quantified in Artocarpus altilia fruit. One of the main compounds was quercetin, which is the major class of flavonoids has been identified and quantified in pulp part of A. altilis fruit of methanol extract. The aim of this study was to evaluate in vitro...

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Main Authors:	Jalal, Tara Kamal, Firus Khan, Al'aina Yuhainis, Natto, Hatim A., Abdull Rasad, Mohammad Syaiful Bahari, Kaderi, Mohd Arifin, Mohammad, Mardhiah, Johan, Muhammad Farid, Omar, Muhammad Nor, Abdul Wahab, Ridhwan
Format:	Article
Language:	English English English
Published:	Taylor & Francis Group 2019
Subjects:	RA644.3 Public Health. Hygiene. Preventive Medicine - Chronic and Noninfectious Diseases and Public Health RM147 Administration of Drugs and Other Therapeutic Agents RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/69643/ http://irep.iium.edu.my/69643/ http://irep.iium.edu.my/69643/ http://irep.iium.edu.my/69643/7/69643%20Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20-In-press.pdf http://irep.iium.edu.my/69643/9/69643%20Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20%20SCOPUS-In-press.pdf http://irep.iium.edu.my/69643/20/69643_Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20of%20Artocarpus%20altilis%20by%20Targeting%20Related%20Genes%20of%20Apoptosis%20and%20Cell%20Cycle_WOS.pdf

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spelling	iium-696432019-07-31T04:17:40Z http://irep.iium.edu.my/69643/ Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines Jalal, Tara Kamal Firus Khan, Al'aina Yuhainis Natto, Hatim A. Abdull Rasad, Mohammad Syaiful Bahari Kaderi, Mohd Arifin Mohammad, Mardhiah Johan, Muhammad Farid Omar, Muhammad Nor Abdul Wahab, Ridhwan RA644.3 Public Health. Hygiene. Preventive Medicine - Chronic and Noninfectious Diseases and Public Health RM147 Administration of Drugs and Other Therapeutic Agents RM300 Drugs and their action Nine phenolic compounds were identified and quantified in Artocarpus altilia fruit. One of the main compounds was quercetin, which is the major class of flavonoids has been identified and quantified in pulp part of A. altilis fruit of methanol extract. The aim of this study was to evaluate in vitro cytotoxic assay. Inhibitory concentration 50% concentration was determined using trypan blue exclusion assay. Apoptosis induction and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell cycle-related regulatory genes were assessed by RT-qPCR study of the methanol extract of pulp part on human lung carcinoma (A549) cell line. A significant increase of cells at G2/M phases was detected (P<0.05). Furthermore, the pulp of the fruit downregulated the expression of antiapoptosis gene BCL-2 and upregulated the expression of pro-apoptosis gene BAX. CASPASE3 was also activated by the fruit, which started a CASPASE-3-depended mitochondrial pathway to induce apoptosis. As the results, the pulp was the most active in terms of all tests, due to high amount of quercetin in pulp part, 78% of total flavonoids. Taken together, these findings suggested that A. altilis induces apoptosis in a mitochondrial-dependent pathway by releasing and upregulating CYTOCHROME C expression and regulates the expression of downstream apoptotic components, including BCL-2 and BAX. Taylor & Francis Group 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69643/7/69643%20Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20-In-press.pdf application/pdf en http://irep.iium.edu.my/69643/9/69643%20Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20%20SCOPUS-In-press.pdf application/pdf en http://irep.iium.edu.my/69643/20/69643_Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20of%20Artocarpus%20altilis%20by%20Targeting%20Related%20Genes%20of%20Apoptosis%20and%20Cell%20Cycle_WOS.pdf Jalal, Tara Kamal and Firus Khan, Al'aina Yuhainis and Natto, Hatim A. and Abdull Rasad, Mohammad Syaiful Bahari and Kaderi, Mohd Arifin and Mohammad, Mardhiah and Johan, Muhammad Farid and Omar, Muhammad Nor and Abdul Wahab, Ridhwan (2019) Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines. Nutrition and Cancer. pp. 1-14. ISSN 0163-5581 E-ISSN 1532-7914 (In Press) https://www.tandfonline.com/doi/abs/10.1080/01635581.2018.1516790 10.1080/01635581.2018.1516790
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RA644.3 Public Health. Hygiene. Preventive Medicine - Chronic and Noninfectious Diseases and Public Health RM147 Administration of Drugs and Other Therapeutic Agents RM300 Drugs and their action
spellingShingle	RA644.3 Public Health. Hygiene. Preventive Medicine - Chronic and Noninfectious Diseases and Public Health RM147 Administration of Drugs and Other Therapeutic Agents RM300 Drugs and their action Jalal, Tara Kamal Firus Khan, Al'aina Yuhainis Natto, Hatim A. Abdull Rasad, Mohammad Syaiful Bahari Kaderi, Mohd Arifin Mohammad, Mardhiah Johan, Muhammad Farid Omar, Muhammad Nor Abdul Wahab, Ridhwan Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines
description	Nine phenolic compounds were identified and quantified in Artocarpus altilia fruit. One of the main compounds was quercetin, which is the major class of flavonoids has been identified and quantified in pulp part of A. altilis fruit of methanol extract. The aim of this study was to evaluate in vitro cytotoxic assay. Inhibitory concentration 50% concentration was determined using trypan blue exclusion assay. Apoptosis induction and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell cycle-related regulatory genes were assessed by RT-qPCR study of the methanol extract of pulp part on human lung carcinoma (A549) cell line. A significant increase of cells at G2/M phases was detected (P<0.05). Furthermore, the pulp of the fruit downregulated the expression of antiapoptosis gene BCL-2 and upregulated the expression of pro-apoptosis gene BAX. CASPASE3 was also activated by the fruit, which started a CASPASE-3-depended mitochondrial pathway to induce apoptosis. As the results, the pulp was the most active in terms of all tests, due to high amount of quercetin in pulp part, 78% of total flavonoids. Taken together, these findings suggested that A. altilis induces apoptosis in a mitochondrial-dependent pathway by releasing and upregulating CYTOCHROME C expression and regulates the expression of downstream apoptotic components, including BCL-2 and BAX.
format	Article
author	Jalal, Tara Kamal Firus Khan, Al'aina Yuhainis Natto, Hatim A. Abdull Rasad, Mohammad Syaiful Bahari Kaderi, Mohd Arifin Mohammad, Mardhiah Johan, Muhammad Farid Omar, Muhammad Nor Abdul Wahab, Ridhwan
author_facet	Jalal, Tara Kamal Firus Khan, Al'aina Yuhainis Natto, Hatim A. Abdull Rasad, Mohammad Syaiful Bahari Kaderi, Mohd Arifin Mohammad, Mardhiah Johan, Muhammad Farid Omar, Muhammad Nor Abdul Wahab, Ridhwan
author_sort	Jalal, Tara Kamal
title	Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines
title_short	Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines
title_full	Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines
title_fullStr	Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines
title_full_unstemmed	Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines
title_sort	identification and quantification of quercetin, a major constituent of artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines
publisher	Taylor & Francis Group
publishDate	2019
url	http://irep.iium.edu.my/69643/ http://irep.iium.edu.my/69643/ http://irep.iium.edu.my/69643/ http://irep.iium.edu.my/69643/7/69643%20Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20-In-press.pdf http://irep.iium.edu.my/69643/9/69643%20Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20%20SCOPUS-In-press.pdf http://irep.iium.edu.my/69643/20/69643_Identification%20and%20Quantification%20of%20Quercetin%2C%20A%20Major%20Constituent%20of%20Artocarpus%20altilis%20by%20Targeting%20Related%20Genes%20of%20Apoptosis%20and%20Cell%20Cycle_WOS.pdf
first_indexed	2023-09-18T21:38:51Z
last_indexed	2023-09-18T21:38:51Z
_version_	1777413003982929920

Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines

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