Comparison of extraction techniques for three Calophyllum species and their antioxidant activity

Calophyllum is a pan-tropical genus belongs to the Guttiferae family and locally known in Malaysia as ‘bintangor’. There has been a continual interest to further investigate the phytochemistry of Calophyllum sp since this genus is a rich source of active secondary metabolites which showed anti-HIV,...

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Main Authors:	Ramli, Siti Norhafizah, Aminudin, Nurul Iman, Ahmad, Farediah, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/69564/ http://irep.iium.edu.my/69564/1/SKAM31%20PROGRAMME%20BOOK.pdf http://irep.iium.edu.my/69564/7/69564.pdf

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recordtype	eprints
spelling	iium-695642019-01-30T04:38:51Z http://irep.iium.edu.my/69564/ Comparison of extraction techniques for three Calophyllum species and their antioxidant activity Ramli, Siti Norhafizah Aminudin, Nurul Iman Ahmad, Farediah Darnis, Deny Susanti QD Chemistry Calophyllum is a pan-tropical genus belongs to the Guttiferae family and locally known in Malaysia as ‘bintangor’. There has been a continual interest to further investigate the phytochemistry of Calophyllum sp since this genus is a rich source of active secondary metabolites which showed anti-HIV, cytotoxicity and antimicrobial properties. This study was conducted to investigate the effect of extraction techniques on the phytochemicals content and antioxidant activity of the barks, leaves and heartwood extracts of three Calophyllum species, C. incrassatum, C. rubiginosum and C. canum. Soxhlet and maceration extraction techniques by using methanol as solvent were chosen in this study. Maceration extraction technique produced higher percentage yield compared to Soxhlet extraction for leaves and barks of the three Calophyllum species. Highest percentage yield was obtained from bark extract of C. canum (21.76%) followed by bark extract of C. rubiginosum (20.24%) and leaves extract of C. rubiginosum (19.34%). Meanwhile, Soxhlet extraction technique gave higher percentage yield compared to maceration technique for heartwood extracts of all samples. The phytochemical screening test revealed all the extracts contain tannin, phenol, flavonoid, terpenes, cardiac glycoside, coumarin and phytosterol. The antioxidant activity of all extracts was tested by determining the Total Phenolic Content and DPPH radical scavenging activity. The highest Total Phenolic Content was obtained from Soxhlet extraction technique. The bark extract of C. canum displayed the highest phenolic content (461.90 mg GAE/g) followed by bark extract of C. incrassatum (394.52 mg GAE/g) and leave extract of C. incrassatum (227.89 mg GAE/g). Meanwhile, the extracts from Soxhlet extraction technique gave higher antioxidant activity compared to maceration extraction. The bark extract of C. canum showed the lowest IC50 value (3.07 µg/mL) followed by bark extract of C. incrassatum (5.12 µg/mL) and leave extract of C. incrassatum (5.93 µg/mL). Pearson’s correlation test showed positive correlation between Total Phenolic Content and DPPH radical scavenging activity. 2018-08-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/69564/1/SKAM31%20PROGRAMME%20BOOK.pdf application/pdf en http://irep.iium.edu.my/69564/7/69564.pdf Ramli, Siti Norhafizah and Aminudin, Nurul Iman and Ahmad, Farediah and Darnis, Deny Susanti (2018) Comparison of extraction techniques for three Calophyllum species and their antioxidant activity. In: International Conference of Analytical Sciences (SKAM31), 17th-19th August 2018, Vistana Hotel, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Ramli, Siti Norhafizah Aminudin, Nurul Iman Ahmad, Farediah Darnis, Deny Susanti Comparison of extraction techniques for three Calophyllum species and their antioxidant activity
description	Calophyllum is a pan-tropical genus belongs to the Guttiferae family and locally known in Malaysia as ‘bintangor’. There has been a continual interest to further investigate the phytochemistry of Calophyllum sp since this genus is a rich source of active secondary metabolites which showed anti-HIV, cytotoxicity and antimicrobial properties. This study was conducted to investigate the effect of extraction techniques on the phytochemicals content and antioxidant activity of the barks, leaves and heartwood extracts of three Calophyllum species, C. incrassatum, C. rubiginosum and C. canum. Soxhlet and maceration extraction techniques by using methanol as solvent were chosen in this study. Maceration extraction technique produced higher percentage yield compared to Soxhlet extraction for leaves and barks of the three Calophyllum species. Highest percentage yield was obtained from bark extract of C. canum (21.76%) followed by bark extract of C. rubiginosum (20.24%) and leaves extract of C. rubiginosum (19.34%). Meanwhile, Soxhlet extraction technique gave higher percentage yield compared to maceration technique for heartwood extracts of all samples. The phytochemical screening test revealed all the extracts contain tannin, phenol, flavonoid, terpenes, cardiac glycoside, coumarin and phytosterol. The antioxidant activity of all extracts was tested by determining the Total Phenolic Content and DPPH radical scavenging activity. The highest Total Phenolic Content was obtained from Soxhlet extraction technique. The bark extract of C. canum displayed the highest phenolic content (461.90 mg GAE/g) followed by bark extract of C. incrassatum (394.52 mg GAE/g) and leave extract of C. incrassatum (227.89 mg GAE/g). Meanwhile, the extracts from Soxhlet extraction technique gave higher antioxidant activity compared to maceration extraction. The bark extract of C. canum showed the lowest IC50 value (3.07 µg/mL) followed by bark extract of C. incrassatum (5.12 µg/mL) and leave extract of C. incrassatum (5.93 µg/mL). Pearson’s correlation test showed positive correlation between Total Phenolic Content and DPPH radical scavenging activity.
format	Conference or Workshop Item
author	Ramli, Siti Norhafizah Aminudin, Nurul Iman Ahmad, Farediah Darnis, Deny Susanti
author_facet	Ramli, Siti Norhafizah Aminudin, Nurul Iman Ahmad, Farediah Darnis, Deny Susanti
author_sort	Ramli, Siti Norhafizah
title	Comparison of extraction techniques for three Calophyllum species and their antioxidant activity
title_short	Comparison of extraction techniques for three Calophyllum species and their antioxidant activity
title_full	Comparison of extraction techniques for three Calophyllum species and their antioxidant activity
title_fullStr	Comparison of extraction techniques for three Calophyllum species and their antioxidant activity
title_full_unstemmed	Comparison of extraction techniques for three Calophyllum species and their antioxidant activity
title_sort	comparison of extraction techniques for three calophyllum species and their antioxidant activity
publishDate	2018
url	http://irep.iium.edu.my/69564/ http://irep.iium.edu.my/69564/1/SKAM31%20PROGRAMME%20BOOK.pdf http://irep.iium.edu.my/69564/7/69564.pdf
first_indexed	2023-09-18T21:38:45Z
last_indexed	2023-09-18T21:38:45Z
_version_	1777412997709299712

Comparison of extraction techniques for three Calophyllum species and their antioxidant activity

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