Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)

Studies on bacterial growth pattern from the conventional approach are defective due to their failure to explain the interactions or simply the complementary effects of the factors influencing the bacterial growth. In this study, the individual and collaborative effects of Pseudomonas putida growth...

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Main Authors:	Ahmad, Mani Malam, Mohd Azoddein, Abd. Aziz, Jami, Mohammed Saedi
Format:	Article
Language:	English
Published:	IIUM Press 2018
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/69543/ http://irep.iium.edu.my/69543/ http://irep.iium.edu.my/69543/1/69543_Factorial%20analysis%20of%20growth%20parameters%20for%20mesophilic%20bacterium_article.pdf

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spelling	iium-695432019-02-15T07:52:50Z http://irep.iium.edu.my/69543/ Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128) Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Jami, Mohammed Saedi TP155 Chemical engineering Studies on bacterial growth pattern from the conventional approach are defective due to their failure to explain the interactions or simply the complementary effects of the factors influencing the bacterial growth. In this study, the individual and collaborative effects of Pseudomonas putida growth variables were evaluated using a 2-level fractional factorial design of experiment (FFDOE). The growth of the organism was found to respond remarkably to different concentrations of nutrient media (carbon source) and the other independent variables. Factorial models were developed from the experimental design to study the individual and interactive effects of the studied parameters on the response. The studied parameters and their levels were as follows: nutrient concentration (4-16 g/L), acclimatization time (24-72 hrs), agitation (140-200 rpm), and temperature (30-40oC). These parameters were statistically validated using analysis of variance (ANOVA) and the results revealed that the model terms were statistically significant with an Fvalue of 415.17 at P <0.002. The growth factor with the most influence (positive) on the response was the nutrient concentration. The level of the parameters influence on the response was in the order of nutrient concentration > temperature > nutrient concentration versus temperature > agitation > nutrient concentration versus agitation. Based on the R2 and the adjusted R2 values of >95%, the estimated variables showed a high degree of relationship between the observed and the predicted values; thus, the predictive ability of the models was suggested. It could, therefore, be concluded that nutrient concentration, temperature, and agitation can greatly influence the growth of P. putida within a specific range. IIUM Press 2018-12-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69543/1/69543_Factorial%20analysis%20of%20growth%20parameters%20for%20mesophilic%20bacterium_article.pdf Ahmad, Mani Malam and Mohd Azoddein, Abd. Aziz and Jami, Mohammed Saedi (2018) Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128). Biological And Natural Resources Engineering Journal, 01 (01). pp. 50-57. E-ISSN 2637-0719 http://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/9/6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Jami, Mohammed Saedi Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)
description	Studies on bacterial growth pattern from the conventional approach are defective due to their failure to explain the interactions or simply the complementary effects of the factors influencing the bacterial growth. In this study, the individual and collaborative effects of Pseudomonas putida growth variables were evaluated using a 2-level fractional factorial design of experiment (FFDOE). The growth of the organism was found to respond remarkably to different concentrations of nutrient media (carbon source) and the other independent variables. Factorial models were developed from the experimental design to study the individual and interactive effects of the studied parameters on the response. The studied parameters and their levels were as follows: nutrient concentration (4-16 g/L), acclimatization time (24-72 hrs), agitation (140-200 rpm), and temperature (30-40oC). These parameters were statistically validated using analysis of variance (ANOVA) and the results revealed that the model terms were statistically significant with an Fvalue of 415.17 at P <0.002. The growth factor with the most influence (positive) on the response was the nutrient concentration. The level of the parameters influence on the response was in the order of nutrient concentration > temperature > nutrient concentration versus temperature > agitation > nutrient concentration versus agitation. Based on the R2 and the adjusted R2 values of >95%, the estimated variables showed a high degree of relationship between the observed and the predicted values; thus, the predictive ability of the models was suggested. It could, therefore, be concluded that nutrient concentration, temperature, and agitation can greatly influence the growth of P. putida within a specific range.
format	Article
author	Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Jami, Mohammed Saedi
author_facet	Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Jami, Mohammed Saedi
author_sort	Ahmad, Mani Malam
title	Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)
title_short	Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)
title_full	Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)
title_fullStr	Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)
title_full_unstemmed	Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)
title_sort	factorial analysis of growth parameters for mesophilic bacterium , pseudomonas putida (atcc 49128)
publisher	IIUM Press
publishDate	2018
url	http://irep.iium.edu.my/69543/ http://irep.iium.edu.my/69543/ http://irep.iium.edu.my/69543/1/69543_Factorial%20analysis%20of%20growth%20parameters%20for%20mesophilic%20bacterium_article.pdf
first_indexed	2023-09-18T21:38:43Z
last_indexed	2023-09-18T21:38:43Z
_version_	1777412995363635200

Factorial analysis of growth parameters for mesophilic bacterium , Pseudomonas putida (ATCC 49128)

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