Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers

Our previous study showed that miR-101 and miR-744 were deregulated in nasopharyngeal carcinoma (NPC) tissues as compared to control tissues with certain diagnostic performance. Thus, we would like to observe the expression of both miRNAs in plasma of NPC patients as compared to control subjects and...

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Main Authors:	Azmir, Ahmad, Mohan, Vijaya, A., Sivanesan, Tang, Lu Ping, Tolos, Siti Marponga, Tumian, Afidalina, Abdullah, Kahairi, Kaderi, Mohd Arifin
Format:	Article
Language:	English
Published:	Innovare Academic Sciences 2018
Subjects:	Q Science (General) R Medicine (General) RB Pathology
Online Access:	http://irep.iium.edu.my/69281/ http://irep.iium.edu.my/69281/ http://irep.iium.edu.my/69281/1/publication%202.pdf

id	iium-69281
recordtype	eprints
spelling	iium-692812019-01-17T04:10:26Z http://irep.iium.edu.my/69281/ Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers Azmir, Ahmad Mohan, Vijaya A., Sivanesan Tang, Lu Ping Tolos, Siti Marponga Tumian, Afidalina Abdullah, Kahairi Kaderi, Mohd Arifin Q Science (General) R Medicine (General) RB Pathology Our previous study showed that miR-101 and miR-744 were deregulated in nasopharyngeal carcinoma (NPC) tissues as compared to control tissues with certain diagnostic performance. Thus, we would like to observe the expression of both miRNAs in plasma of NPC patients as compared to control subjects and analyse their diagnostic performance. Plasma were collected from 33 NPC patients and 33 control subjects and subjected to total RNA extraction. Reverse transcription, pre-amplification and quantitative polymerase chain reaction (qPCR) were performed on the total RNA extract using Taqman microRNA Assays. Fold-change of the miRNAs were calculated using 2-ΔΔCq formula and statistical analyses were performed using SPSS version 20 software. The qPCR results showed that miR-101 and miR-744 (p>0.05) were up-regulated in NPC as compared to control plasma. Receiver operating characteristic curve analysis showed that area under curve (AUC) for miR-101, miR-744 and combination of miR-101 and miR744 were 0.374, 0.410 and 0.437, respectively, in discriminating NPC patients from control subjects, but they have AUC of 0.647, 0.69 and 0.599, respectively, in discriminating early from late stage NPC. The up-regulation of miR-101 and miR-744 have been reported in previous studies indicating the consistency of our findings. Although we found that they have poor diagnostic values in plasma for discriminating NPC patients from control subjects, they have better diagnostic values in discriminating early from late stage NPC. Therefore, we have successfully reported the up- regulation of miR-101 and miR-744 in plasma of NPC with respective diagnostic performance. Innovare Academic Sciences 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69281/1/publication%202.pdf Azmir, Ahmad and Mohan, Vijaya and A., Sivanesan and Tang, Lu Ping and Tolos, Siti Marponga and Tumian, Afidalina and Abdullah, Kahairi and Kaderi, Mohd Arifin (2018) Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers. Asian Journal of Pharmaceutical and Clinical Research, 11 (9). p. 14. ISSN 0974-2441 E-ISSN 2455-3891 https://innovareacademics.in/journals/index.php/ijpps/issue/archive
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) R Medicine (General) RB Pathology
spellingShingle	Q Science (General) R Medicine (General) RB Pathology Azmir, Ahmad Mohan, Vijaya A., Sivanesan Tang, Lu Ping Tolos, Siti Marponga Tumian, Afidalina Abdullah, Kahairi Kaderi, Mohd Arifin Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers
description	Our previous study showed that miR-101 and miR-744 were deregulated in nasopharyngeal carcinoma (NPC) tissues as compared to control tissues with certain diagnostic performance. Thus, we would like to observe the expression of both miRNAs in plasma of NPC patients as compared to control subjects and analyse their diagnostic performance. Plasma were collected from 33 NPC patients and 33 control subjects and subjected to total RNA extraction. Reverse transcription, pre-amplification and quantitative polymerase chain reaction (qPCR) were performed on the total RNA extract using Taqman microRNA Assays. Fold-change of the miRNAs were calculated using 2-ΔΔCq formula and statistical analyses were performed using SPSS version 20 software. The qPCR results showed that miR-101 and miR-744 (p>0.05) were up-regulated in NPC as compared to control plasma. Receiver operating characteristic curve analysis showed that area under curve (AUC) for miR-101, miR-744 and combination of miR-101 and miR744 were 0.374, 0.410 and 0.437, respectively, in discriminating NPC patients from control subjects, but they have AUC of 0.647, 0.69 and 0.599, respectively, in discriminating early from late stage NPC. The up-regulation of miR-101 and miR-744 have been reported in previous studies indicating the consistency of our findings. Although we found that they have poor diagnostic values in plasma for discriminating NPC patients from control subjects, they have better diagnostic values in discriminating early from late stage NPC. Therefore, we have successfully reported the up- regulation of miR-101 and miR-744 in plasma of NPC with respective diagnostic performance.
format	Article
author	Azmir, Ahmad Mohan, Vijaya A., Sivanesan Tang, Lu Ping Tolos, Siti Marponga Tumian, Afidalina Abdullah, Kahairi Kaderi, Mohd Arifin
author_facet	Azmir, Ahmad Mohan, Vijaya A., Sivanesan Tang, Lu Ping Tolos, Siti Marponga Tumian, Afidalina Abdullah, Kahairi Kaderi, Mohd Arifin
author_sort	Azmir, Ahmad
title	Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers
title_short	Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers
title_full	Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers
title_fullStr	Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers
title_full_unstemmed	Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers
title_sort	expression of circulating mir-101 and mir-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers
publisher	Innovare Academic Sciences
publishDate	2018
url	http://irep.iium.edu.my/69281/ http://irep.iium.edu.my/69281/ http://irep.iium.edu.my/69281/1/publication%202.pdf
first_indexed	2023-09-18T21:38:20Z
last_indexed	2023-09-18T21:38:20Z
_version_	1777412971424645120

Expression of circulating miR-101 and miR-744 in nasopharyngeal carcinoma and their performance as diagnostic biomarkers

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