Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization

Lipid vesicles in the nano range with ionic and nonionic surfactants are known as transfersomes. The presence of surfactant in the bilayer structure makes the vesicles very flexible in nature and helps them to permeate through the stratum corneum. The purpose of this research was to develop and char...

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Main Authors:	Mahmood, Sayed, Chatterjee, Bappaditya, Mandal, Uttam Kumar
Format:	Article
Language:	English
Published:	Medwin 2018
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/69268/ http://irep.iium.edu.my/69268/ http://irep.iium.edu.my/69268/ http://irep.iium.edu.my/69268/2/69268_Nano%20transfersomes%20vesicles%20of%20raloxifene%20HCl%20with%20sorbitan%2080_article.pdf

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recordtype	eprints
spelling	iium-692682019-02-18T01:46:07Z http://irep.iium.edu.my/69268/ Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization Mahmood, Sayed Chatterjee, Bappaditya Mandal, Uttam Kumar RS192 Materia Medica-Pharmaceutical Technology Lipid vesicles in the nano range with ionic and nonionic surfactants are known as transfersomes. The presence of surfactant in the bilayer structure makes the vesicles very flexible in nature and helps them to permeate through the stratum corneum. The purpose of this research was to develop and characterize a transfersomal formulation of raloxifene HCl to deliver it into systemic circulation through the transdermal route. The transfersomal formulation was prepared by the rotary evaporation method with phospholipon 90G and sorbitan 80. The particle size, zeta potential and polydispersity index (PDI) of the formulation were measured. The drug entrapment efficiency (EE%) of the vesicles was determined by an indirect ultracentrifugation method. Differential scanning calorimetry (DSC), ex-vivo skin permeation study, field emission scanning electron microscope (FESEM), high resolution transmission electron microscope (HRTEM) and confocal laser scanning microscopy (CLSM) study were carried out as parts of advanced characterization of the developed formulation. The vesicles were found to have an average particle size of 95.1±1.05nm with a PDI value of 0.162±0.01 and zeta potential of +17.62±0.29 mV. EE% was recorded up to 90.9±1.15. Transdermal flux (J = 4.66±0.79 μg/cm2hr) of the developed formulation showed a favorable value required for the formulation efficacy. FESEM and TEM study results proved the spherical and round structures of the vesicles. DSC showed that the raloxifene was in the non-crystal form and was enclosed in the lipid bilayer. CLSM study proved the distribution of the drug in the stratum corneum, viable epidermis and dermis with high fluorescence intensity. The developed nano transfersomes of raloxifene HCl with sorbitan 80 showed encouraging results and can be further investigated for in vivo efficacy. Medwin 2018-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69268/2/69268_Nano%20transfersomes%20vesicles%20of%20raloxifene%20HCl%20with%20sorbitan%2080_article.pdf Mahmood, Sayed and Chatterjee, Bappaditya and Mandal, Uttam Kumar (2018) Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization. Bioequivalence & Bioavailability International Journal (BEBA), 2 (1). pp. 1-7. ISSN 2578-4803 https://medwinpublishers.com/BEBA/BEBA16000121.pdf 10.23880/beba-16000121
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Mahmood, Sayed Chatterjee, Bappaditya Mandal, Uttam Kumar Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization
description	Lipid vesicles in the nano range with ionic and nonionic surfactants are known as transfersomes. The presence of surfactant in the bilayer structure makes the vesicles very flexible in nature and helps them to permeate through the stratum corneum. The purpose of this research was to develop and characterize a transfersomal formulation of raloxifene HCl to deliver it into systemic circulation through the transdermal route. The transfersomal formulation was prepared by the rotary evaporation method with phospholipon 90G and sorbitan 80. The particle size, zeta potential and polydispersity index (PDI) of the formulation were measured. The drug entrapment efficiency (EE%) of the vesicles was determined by an indirect ultracentrifugation method. Differential scanning calorimetry (DSC), ex-vivo skin permeation study, field emission scanning electron microscope (FESEM), high resolution transmission electron microscope (HRTEM) and confocal laser scanning microscopy (CLSM) study were carried out as parts of advanced characterization of the developed formulation. The vesicles were found to have an average particle size of 95.1±1.05nm with a PDI value of 0.162±0.01 and zeta potential of +17.62±0.29 mV. EE% was recorded up to 90.9±1.15. Transdermal flux (J = 4.66±0.79 μg/cm2hr) of the developed formulation showed a favorable value required for the formulation efficacy. FESEM and TEM study results proved the spherical and round structures of the vesicles. DSC showed that the raloxifene was in the non-crystal form and was enclosed in the lipid bilayer. CLSM study proved the distribution of the drug in the stratum corneum, viable epidermis and dermis with high fluorescence intensity. The developed nano transfersomes of raloxifene HCl with sorbitan 80 showed encouraging results and can be further investigated for in vivo efficacy.
format	Article
author	Mahmood, Sayed Chatterjee, Bappaditya Mandal, Uttam Kumar
author_facet	Mahmood, Sayed Chatterjee, Bappaditya Mandal, Uttam Kumar
author_sort	Mahmood, Sayed
title	Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization
title_short	Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization
title_full	Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization
title_fullStr	Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization
title_full_unstemmed	Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization
title_sort	nano transfersomes vesicles of raloxifene hcl with sorbitan 80: formulation and characterization
publisher	Medwin
publishDate	2018
url	http://irep.iium.edu.my/69268/ http://irep.iium.edu.my/69268/ http://irep.iium.edu.my/69268/ http://irep.iium.edu.my/69268/2/69268_Nano%20transfersomes%20vesicles%20of%20raloxifene%20HCl%20with%20sorbitan%2080_article.pdf
first_indexed	2023-09-18T21:38:19Z
last_indexed	2023-09-18T21:38:19Z
_version_	1777412970135945216

Nano transfersomes vesicles of raloxifene HCl with sorbitan 80: formulation and characterization

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