Chronic organic arsenic induced liver ultra structural damage

Introduction: Inorganic arsenic is one of the environmental toxins that has been associated with increased risk of cardiovascular diseases and vascular contributions to liver diseases. It has generally been thought to be more toxic than organic arsenic. In human liver, inorganic arsenic promotes vas...

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Main Authors:	Saharudin, Shahida, A. Talib, Norlelawati, Abdullah, Nor Zamzila, Ab Rahman, Jamalludin, Buyong, Zunariah
Format:	Article
Language:	English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2018
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/69260/ http://irep.iium.edu.my/69260/ http://irep.iium.edu.my/69260/1/MRS2018-38%20Chronic%20Organic%20Arsenic%20Induced%20Liver%20Ultrastructural%20Damage%20IMJM%20Vol%2017%20Supp%20Issue%201.pdf

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spelling	iium-692602019-01-18T02:03:38Z http://irep.iium.edu.my/69260/ Chronic organic arsenic induced liver ultra structural damage Saharudin, Shahida A. Talib, Norlelawati Abdullah, Nor Zamzila Ab Rahman, Jamalludin Buyong, Zunariah R Medicine (General) Introduction: Inorganic arsenic is one of the environmental toxins that has been associated with increased risk of cardiovascular diseases and vascular contributions to liver diseases. It has generally been thought to be more toxic than organic arsenic. In human liver, inorganic arsenic promotes vascular remodelling, portal fibrosis and hypertension. The purpose of the current study was to determine whether chronic exposure to organic arsenic impair liver ultrastructure as in inorganic arsenic exposure. Materials and Methods: Twenty eight male SpragueDawley rats were divided into 2 groups with their own control group. They received oral intubation of monosodium- methylarsonate (MSMA) at 63.30 mg/kg body weight for 4 and 6 months duration respectively while the control groups received distilled water. The liver of euthanized rats were perfused- fixed with glutaraldehyde for transmission electron microscopy processing. Results: TEM revealed a marked reduction in the number of mitochondria in both treatment groups. Some typical features of apoptosis are present with pyknotic nuclei of hepatocytes and disintegrated hepatic cytoplasm in 4- month groups. The microvilli of hepatocyte are almost completely absent with the presence of many fibre bundles (collagen fibres) widening the space of Disse. In 6-month treatment group, disintegrated cytoplasms are more prominent with degrading mitochondria of varying stages. Liver sinusoidal endothelial cells (LSEC) of 6-month treatment group are noted to have chromatin condensation with few caveolae seen. Conclusion: Chronic exposure of MSMA leads to necrotic changes of hepatocytes and apoptotic changes in LSEC and collagenisation in the space of Disse. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2018-12-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69260/1/MRS2018-38%20Chronic%20Organic%20Arsenic%20Induced%20Liver%20Ultrastructural%20Damage%20IMJM%20Vol%2017%20Supp%20Issue%201.pdf Saharudin, Shahida and A. Talib, Norlelawati and Abdullah, Nor Zamzila and Ab Rahman, Jamalludin and Buyong, Zunariah (2018) Chronic organic arsenic induced liver ultra structural damage. International Medical Journal Malaysia, 17 (Supplementary Issue No 1). p. 37. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/vol_17_no_3_supp_1/MRS2018-38.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Saharudin, Shahida A. Talib, Norlelawati Abdullah, Nor Zamzila Ab Rahman, Jamalludin Buyong, Zunariah Chronic organic arsenic induced liver ultra structural damage
description	Introduction: Inorganic arsenic is one of the environmental toxins that has been associated with increased risk of cardiovascular diseases and vascular contributions to liver diseases. It has generally been thought to be more toxic than organic arsenic. In human liver, inorganic arsenic promotes vascular remodelling, portal fibrosis and hypertension. The purpose of the current study was to determine whether chronic exposure to organic arsenic impair liver ultrastructure as in inorganic arsenic exposure. Materials and Methods: Twenty eight male SpragueDawley rats were divided into 2 groups with their own control group. They received oral intubation of monosodium- methylarsonate (MSMA) at 63.30 mg/kg body weight for 4 and 6 months duration respectively while the control groups received distilled water. The liver of euthanized rats were perfused- fixed with glutaraldehyde for transmission electron microscopy processing. Results: TEM revealed a marked reduction in the number of mitochondria in both treatment groups. Some typical features of apoptosis are present with pyknotic nuclei of hepatocytes and disintegrated hepatic cytoplasm in 4- month groups. The microvilli of hepatocyte are almost completely absent with the presence of many fibre bundles (collagen fibres) widening the space of Disse. In 6-month treatment group, disintegrated cytoplasms are more prominent with degrading mitochondria of varying stages. Liver sinusoidal endothelial cells (LSEC) of 6-month treatment group are noted to have chromatin condensation with few caveolae seen. Conclusion: Chronic exposure of MSMA leads to necrotic changes of hepatocytes and apoptotic changes in LSEC and collagenisation in the space of Disse.
format	Article
author	Saharudin, Shahida A. Talib, Norlelawati Abdullah, Nor Zamzila Ab Rahman, Jamalludin Buyong, Zunariah
author_facet	Saharudin, Shahida A. Talib, Norlelawati Abdullah, Nor Zamzila Ab Rahman, Jamalludin Buyong, Zunariah
author_sort	Saharudin, Shahida
title	Chronic organic arsenic induced liver ultra structural damage
title_short	Chronic organic arsenic induced liver ultra structural damage
title_full	Chronic organic arsenic induced liver ultra structural damage
title_fullStr	Chronic organic arsenic induced liver ultra structural damage
title_full_unstemmed	Chronic organic arsenic induced liver ultra structural damage
title_sort	chronic organic arsenic induced liver ultra structural damage
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/69260/ http://irep.iium.edu.my/69260/ http://irep.iium.edu.my/69260/1/MRS2018-38%20Chronic%20Organic%20Arsenic%20Induced%20Liver%20Ultrastructural%20Damage%20IMJM%20Vol%2017%20Supp%20Issue%201.pdf
first_indexed	2023-09-18T21:38:18Z
last_indexed	2023-09-18T21:38:18Z
_version_	1777412969429204992

Chronic organic arsenic induced liver ultra structural damage

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