Lipase immobilization on fibers grafted with polyglycidyl methachrylate

Lipase enzyme originated from wheat germ was immobilized on nylon -6- grafted with polyglycidyl methachrylate (PGMA). The immobilization of enzyme experiments were designed and studied using face centred central composite design (FCCCD) under response surface methodology (RSM). Prior to immobilizati...

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Main Authors:	Alkhatib, Ma'an Fahmi Rashid, Bahrudin, Nik Adlin, Mohd. Salleh, Hamzah, Nasef, Mohamed Mahmoud, TING, TEO M.
Format:	Article
Language:	English English English
Published:	IIUM Press, International Islamic University Malaysia 2019
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/69239/ http://irep.iium.edu.my/69239/ http://irep.iium.edu.my/69239/ http://irep.iium.edu.my/69239/3/Alkhatib%2C%20LIPASE%20IMMOBILIZATION%20ON%20FIBE..ACCEPT.pdf http://irep.iium.edu.my/69239/9/69239_Lipase%20immobilization.pdf http://irep.iium.edu.my/69239/15/69239_Lipase%20immobilization%20on%20fibers_scopus.pdf

id	iium-69239
recordtype	eprints
spelling	iium-692392019-11-24T16:26:14Z http://irep.iium.edu.my/69239/ Lipase immobilization on fibers grafted with polyglycidyl methachrylate Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud TING, TEO M. TP155 Chemical engineering TP248.13 Biotechnology Lipase enzyme originated from wheat germ was immobilized on nylon -6- grafted with polyglycidyl methachrylate (PGMA). The immobilization of enzyme experiments were designed and studied using face centred central composite design (FCCCD) under response surface methodology (RSM). Prior to immobilization, the polymer was activated with diethyl amine/ethanol to introduce amine functional group to facilitate covalent bonding with the enzyme. The immobilized and free enzymes were characterized for effect of temperature and pH on enzyme activity, stability, storage and reusability as well as kinetics studies. ANOVA revealed that optimum lipase activity of 0.287 U/ml was achieved at immobilization time of 5 h, pH of 6 and 1.0 mg/ml for enzyme concentration. The optimum temperatures and pH for immobilized and free enzymes were 45 °C and 35 °C, and 8 and 7, respectively. The immobilized enzyme showed higher stability compared to free enzyme. The immobilized enzyme retained 18% of its activity after being recycled for 8 times. In storage stability test, immobilized lipase was able to retain 70% of its activity after being stored for 30 days, while free enzyme activity dropped to 15 % after 20 days of storage. IIUM Press, International Islamic University Malaysia 2019-06-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69239/3/Alkhatib%2C%20LIPASE%20IMMOBILIZATION%20ON%20FIBE..ACCEPT.pdf application/pdf en http://irep.iium.edu.my/69239/9/69239_Lipase%20immobilization.pdf application/pdf en http://irep.iium.edu.my/69239/15/69239_Lipase%20immobilization%20on%20fibers_scopus.pdf Alkhatib, Ma'an Fahmi Rashid and Bahrudin, Nik Adlin and Mohd. Salleh, Hamzah and Nasef, Mohamed Mahmoud and TING, TEO M. (2019) Lipase immobilization on fibers grafted with polyglycidyl methachrylate. IIUM Engineering Journal, 20 (1). pp. 12-23. ISSN 1511-788X E-ISSN 2289-7860 https://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/1002 https://doi.org/10.31436/iiumej.v20i1.1002
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud TING, TEO M. Lipase immobilization on fibers grafted with polyglycidyl methachrylate
description	Lipase enzyme originated from wheat germ was immobilized on nylon -6- grafted with polyglycidyl methachrylate (PGMA). The immobilization of enzyme experiments were designed and studied using face centred central composite design (FCCCD) under response surface methodology (RSM). Prior to immobilization, the polymer was activated with diethyl amine/ethanol to introduce amine functional group to facilitate covalent bonding with the enzyme. The immobilized and free enzymes were characterized for effect of temperature and pH on enzyme activity, stability, storage and reusability as well as kinetics studies. ANOVA revealed that optimum lipase activity of 0.287 U/ml was achieved at immobilization time of 5 h, pH of 6 and 1.0 mg/ml for enzyme concentration. The optimum temperatures and pH for immobilized and free enzymes were 45 °C and 35 °C, and 8 and 7, respectively. The immobilized enzyme showed higher stability compared to free enzyme. The immobilized enzyme retained 18% of its activity after being recycled for 8 times. In storage stability test, immobilized lipase was able to retain 70% of its activity after being stored for 30 days, while free enzyme activity dropped to 15 % after 20 days of storage.
format	Article
author	Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud TING, TEO M.
author_facet	Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud TING, TEO M.
author_sort	Alkhatib, Ma'an Fahmi Rashid
title	Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_short	Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_full	Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_fullStr	Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_full_unstemmed	Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_sort	lipase immobilization on fibers grafted with polyglycidyl methachrylate
publisher	IIUM Press, International Islamic University Malaysia
publishDate	2019
url	http://irep.iium.edu.my/69239/ http://irep.iium.edu.my/69239/ http://irep.iium.edu.my/69239/ http://irep.iium.edu.my/69239/3/Alkhatib%2C%20LIPASE%20IMMOBILIZATION%20ON%20FIBE..ACCEPT.pdf http://irep.iium.edu.my/69239/9/69239_Lipase%20immobilization.pdf http://irep.iium.edu.my/69239/15/69239_Lipase%20immobilization%20on%20fibers_scopus.pdf
first_indexed	2023-09-18T21:38:17Z
last_indexed	2023-09-18T21:38:17Z
_version_	1777412967816495104

Lipase immobilization on fibers grafted with polyglycidyl methachrylate

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