Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties

Antibacterial agents from natural sources have been suggested as alternatives for treating infectious diseases due to their low side effects. Flaxseed (Linum usitatissimum) exhibits some antibacterial properties, but its effects against oral pathogens are poorly understood. This study investigated f...

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Main Authors:	Fadzir, Ummu Afifah, Mokhtar@Makhtar, Khairani Idah, Mustafa Al-Ahmad, Basma Ezzat, Darnis, Deny Susanti
Format:	Article
Language:	English English
Published:	University Indonesia 2018
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/69154/ http://irep.iium.edu.my/69154/ http://irep.iium.edu.my/69154/ http://irep.iium.edu.my/69154/7/69154%20Evaluation%20of%20bioactive%20compounds%20in%20different%20extracts.pdf http://irep.iium.edu.my/69154/8/69154%20Evaluation%20of%20bioactive%20compounds%20in%20different%20extracts%20WOS.pdf

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recordtype	eprints
spelling	iium-691542019-01-29T08:17:26Z http://irep.iium.edu.my/69154/ Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti QD Chemistry Antibacterial agents from natural sources have been suggested as alternatives for treating infectious diseases due to their low side effects. Flaxseed (Linum usitatissimum) exhibits some antibacterial properties, but its effects against oral pathogens are poorly understood. This study investigated flaxseed extracts’ antibacterial effects against such pathogens. Methods: Non-polar and polar extracts of flaxseed, with n-hexane, dichloromethane (DCM) and methanol (MeOH), were prepared by sequential Soxhlet extraction. All extracts were qualitatively screened through gas chromatography–mass spectrometry to detect bioactive compounds. Antibacterial activities of flaxseed extracts with different volumes per disc (Oxoid, Badhoevedorp, Netherlands) (5, 7, 10, 12 and 15 μL/disc) were evaluated against four different oral pathogens: Streptococcus pyogenes, Streptococcus mutans, Lactobacillus casei and Enterococcus faecalis, using the disc diffusion method. Flaxseed extracts’ inhibitory activities against the tested oral pathogens were examined by measuring the inhibition zone diameter. Results: Polar extract (methanolic extract) demonstrated significant antibacterial activity (p<0.01) against all tested oral pathogens, with inhibition zones comparable to those for penicillin. In contrast, n- hexane and DCM extracts demonstrated variable antibacterial activities. Conclusions: This study suggests that Linum usitatissimum methanolic extract exhibited the best inhibitory activity on all tested strains compared to the other extracts. University Indonesia 2018-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69154/7/69154%20Evaluation%20of%20bioactive%20compounds%20in%20different%20extracts.pdf application/pdf en http://irep.iium.edu.my/69154/8/69154%20Evaluation%20of%20bioactive%20compounds%20in%20different%20extracts%20WOS.pdf Fadzir, Ummu Afifah and Mokhtar@Makhtar, Khairani Idah and Mustafa Al-Ahmad, Basma Ezzat and Darnis, Deny Susanti (2018) Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties. Makara Journal Health Research, 22 (3). pp. 121-127. ISSN 2356-3664 E-ISSN 2356-3656 http://journal.ui.ac.id/index.php/health/article/view/10181/pdf 10.7454/msk.v22i3.10181
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties
description	Antibacterial agents from natural sources have been suggested as alternatives for treating infectious diseases due to their low side effects. Flaxseed (Linum usitatissimum) exhibits some antibacterial properties, but its effects against oral pathogens are poorly understood. This study investigated flaxseed extracts’ antibacterial effects against such pathogens. Methods: Non-polar and polar extracts of flaxseed, with n-hexane, dichloromethane (DCM) and methanol (MeOH), were prepared by sequential Soxhlet extraction. All extracts were qualitatively screened through gas chromatography–mass spectrometry to detect bioactive compounds. Antibacterial activities of flaxseed extracts with different volumes per disc (Oxoid, Badhoevedorp, Netherlands) (5, 7, 10, 12 and 15 μL/disc) were evaluated against four different oral pathogens: Streptococcus pyogenes, Streptococcus mutans, Lactobacillus casei and Enterococcus faecalis, using the disc diffusion method. Flaxseed extracts’ inhibitory activities against the tested oral pathogens were examined by measuring the inhibition zone diameter. Results: Polar extract (methanolic extract) demonstrated significant antibacterial activity (p<0.01) against all tested oral pathogens, with inhibition zones comparable to those for penicillin. In contrast, n- hexane and DCM extracts demonstrated variable antibacterial activities. Conclusions: This study suggests that Linum usitatissimum methanolic extract exhibited the best inhibitory activity on all tested strains compared to the other extracts.
format	Article
author	Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti
author_facet	Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti
author_sort	Fadzir, Ummu Afifah
title	Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties
title_short	Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties
title_full	Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties
title_fullStr	Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties
title_full_unstemmed	Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties
title_sort	evaluation of bioactive compounds in different extracts of linum usitatissimum and their antibacterial properties
publisher	University Indonesia
publishDate	2018
url	http://irep.iium.edu.my/69154/ http://irep.iium.edu.my/69154/ http://irep.iium.edu.my/69154/ http://irep.iium.edu.my/69154/7/69154%20Evaluation%20of%20bioactive%20compounds%20in%20different%20extracts.pdf http://irep.iium.edu.my/69154/8/69154%20Evaluation%20of%20bioactive%20compounds%20in%20different%20extracts%20WOS.pdf
first_indexed	2023-09-18T21:38:10Z
last_indexed	2023-09-18T21:38:10Z
_version_	1777412960889602048

Evaluation of bioactive compounds in different extracts of Linum usitatissimum and their antibacterial properties

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