Correlation of leptin with clinico-pathological features of breast cancer

Leptin is a multifunctional hormone produced mainly by adipocyte. Leptin and its receptor have long been found associated with breast cancer. Our aim is to investigate the correlation between Leptin/Leptin receptor and the clinico-pathological features of breast cancer. Blood samples for ELISA, ti...

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Main Authors:	Abdulrazzaq, Saad Mohammed, Amjad, Nasser Muhammad, Al-Kubaisi, Muna Khaleel Dheyab, Harun, Norra
Format:	Article
Language:	English
Published:	Innovare Academic Sciences 2018
Subjects:	RB Pathology RD Surgery RG Gynecology and obstetrics
Online Access:	http://irep.iium.edu.my/69147/ http://irep.iium.edu.my/69147/ http://irep.iium.edu.my/69147/1/69147_Correlation%20of%20leptin%20with%20clinico-pathological.pdf

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spelling	iium-691472019-01-23T01:42:21Z http://irep.iium.edu.my/69147/ Correlation of leptin with clinico-pathological features of breast cancer Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Harun, Norra RB Pathology RD Surgery RG Gynecology and obstetrics Leptin is a multifunctional hormone produced mainly by adipocyte. Leptin and its receptor have long been found associated with breast cancer. Our aim is to investigate the correlation between Leptin/Leptin receptor and the clinico-pathological features of breast cancer. Blood samples for ELISA, tissue samples from tumours and adjacent breast tissue were taken from 51 women with breast cancer with a control group of 40 women with negative mammogram. Leptin and Leptin receptor in the tissues were estimated by immunohistochemistry (IHC). They were localized at subcellular level by immunocytochemistry using transmission electron microscopy TEM. Our results showed significant difference in serum leptin level between control and the patient group, but no difference between pre and post-operative levels in the patient group. By IHC, we found that majority of the breast cancer cells studied, stained positively for leptin and leptin receptors. Majority of the patients with distant metastasis were associated with high leptin and leptin receptor expression. TEM views both Leptin and Leptin receptor were found highly concentrated within and around the nucleus of the cancer cells, indicating nucleus is their principal seat of actions. However, presence of high concentration of leptin does not necessarily prove its over-expression, because it could be internalized from outside by leptin receptor in the cells. In contrast, leptin receptor is definitely over-expressed in the ductal breast cancer cells. We conclude that reducing leptin levels, blocking its downstream tissue specific signal transduction, and/or blocking the upstream leptin receptor pathway might help in prevention and therapy of breast cancer. Innovare Academic Sciences 2018-09-24 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69147/1/69147_Correlation%20of%20leptin%20with%20clinico-pathological.pdf Abdulrazzaq, Saad Mohammed and Amjad, Nasser Muhammad and Al-Kubaisi, Muna Khaleel Dheyab and Harun, Norra (2018) Correlation of leptin with clinico-pathological features of breast cancer. Asian Journal of Pharmaceutical and Clinical Research, 11 (9). p. 17. ISSN 0974-2441 E-ISSN 2455-3891 https://innovareacademics.in/journals/index.php/ajpcr/issue/view/373
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology RD Surgery RG Gynecology and obstetrics
spellingShingle	RB Pathology RD Surgery RG Gynecology and obstetrics Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Harun, Norra Correlation of leptin with clinico-pathological features of breast cancer
description	Leptin is a multifunctional hormone produced mainly by adipocyte. Leptin and its receptor have long been found associated with breast cancer. Our aim is to investigate the correlation between Leptin/Leptin receptor and the clinico-pathological features of breast cancer. Blood samples for ELISA, tissue samples from tumours and adjacent breast tissue were taken from 51 women with breast cancer with a control group of 40 women with negative mammogram. Leptin and Leptin receptor in the tissues were estimated by immunohistochemistry (IHC). They were localized at subcellular level by immunocytochemistry using transmission electron microscopy TEM. Our results showed significant difference in serum leptin level between control and the patient group, but no difference between pre and post-operative levels in the patient group. By IHC, we found that majority of the breast cancer cells studied, stained positively for leptin and leptin receptors. Majority of the patients with distant metastasis were associated with high leptin and leptin receptor expression. TEM views both Leptin and Leptin receptor were found highly concentrated within and around the nucleus of the cancer cells, indicating nucleus is their principal seat of actions. However, presence of high concentration of leptin does not necessarily prove its over-expression, because it could be internalized from outside by leptin receptor in the cells. In contrast, leptin receptor is definitely over-expressed in the ductal breast cancer cells. We conclude that reducing leptin levels, blocking its downstream tissue specific signal transduction, and/or blocking the upstream leptin receptor pathway might help in prevention and therapy of breast cancer.
format	Article
author	Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Harun, Norra
author_facet	Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Harun, Norra
author_sort	Abdulrazzaq, Saad Mohammed
title	Correlation of leptin with clinico-pathological features of breast cancer
title_short	Correlation of leptin with clinico-pathological features of breast cancer
title_full	Correlation of leptin with clinico-pathological features of breast cancer
title_fullStr	Correlation of leptin with clinico-pathological features of breast cancer
title_full_unstemmed	Correlation of leptin with clinico-pathological features of breast cancer
title_sort	correlation of leptin with clinico-pathological features of breast cancer
publisher	Innovare Academic Sciences
publishDate	2018
url	http://irep.iium.edu.my/69147/ http://irep.iium.edu.my/69147/ http://irep.iium.edu.my/69147/1/69147_Correlation%20of%20leptin%20with%20clinico-pathological.pdf
first_indexed	2023-09-18T21:38:09Z
last_indexed	2023-09-18T21:38:09Z
_version_	1777412960161890304

Correlation of leptin with clinico-pathological features of breast cancer

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