LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves

Averrhoa bilimbi (family: Oxalidaceae) leaves preparations are used in traditional medicine in Malaysia for treatment of several diseases including diabetes. The objective of this study was to investigate the xanthine oxidase (XO) inhibitory potential of A. bilimbi leaves and identification of its b...

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Main Authors:	Ahmed, Qamar Uddin, Alhassan, Alhassan Muhammad, Sarian, Murni Nazira
Format:	Conference or Workshop Item
Language:	English English English English
Published:	2018
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/69002/ http://irep.iium.edu.my/69002/1/Dr%20Qamar%20U.%20Ahmed_PP_CU%2025-26%20Aug%202018.pdf http://irep.iium.edu.my/69002/2/Certificate%20of%20Partcipation.pdf http://irep.iium.edu.my/69002/15/69002-4thPRC2018-1_Abstract%20Final.pdf http://irep.iium.edu.my/69002/16/4IPRC.pdf

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recordtype	eprints
spelling	iium-690022018-12-28T03:43:03Z http://irep.iium.edu.my/69002/ LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Sarian, Murni Nazira Q Science (General) Averrhoa bilimbi (family: Oxalidaceae) leaves preparations are used in traditional medicine in Malaysia for treatment of several diseases including diabetes. The objective of this study was to investigate the xanthine oxidase (XO) inhibitory potential of A. bilimbi leaves and identification of its bioactive constituents through LC-MS-Q-TOF analysis. A. bilimbi leaves crude methanol extract was fractionated with hexane, chloroform and n-butanol. The fractions at different concentrations were subjected to XO inhibitory activity investigation. LC-MS-Q-TOF profiling of the most active fraction was carried out to identify the active compound. Molecular docking was used to investigate the potential inhibitory effect of the active compounds identified through LC-MS-Q-TOF analysis. Results for XO assay demonstrated for n-butanol fraction to display significant inhibitory activity (IC50: 64.84 ± 3.93 μg/mL). However, hexane and chloroform fractions did not show good inhibitory activity. (Z)-Resveratrol-3,4ʹ-β-glucoside and afzelechin-3-O-α-L-rhamnopyranoside were identified in n-butanol fraction through LC-MS-Q-TOF analysis as the possible metabolites responsible for XO inhibitory activity of the n-butanol fraction. Further scientific effort is, however, needed to obtain such bioactive compounds in pure form for complete pharmacological evaluations. 2018-08-24 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/69002/1/Dr%20Qamar%20U.%20Ahmed_PP_CU%2025-26%20Aug%202018.pdf application/pdf en http://irep.iium.edu.my/69002/2/Certificate%20of%20Partcipation.pdf application/pdf en http://irep.iium.edu.my/69002/15/69002-4thPRC2018-1_Abstract%20Final.pdf application/pdf en http://irep.iium.edu.my/69002/16/4IPRC.pdf Ahmed, Qamar Uddin and Alhassan, Alhassan Muhammad and Sarian, Murni Nazira (2018) LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves. In: 4th International Pharmaceutical Research Conference 2018, 25th-26th August 2018, Cyberjaya, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Sarian, Murni Nazira LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves
description	Averrhoa bilimbi (family: Oxalidaceae) leaves preparations are used in traditional medicine in Malaysia for treatment of several diseases including diabetes. The objective of this study was to investigate the xanthine oxidase (XO) inhibitory potential of A. bilimbi leaves and identification of its bioactive constituents through LC-MS-Q-TOF analysis. A. bilimbi leaves crude methanol extract was fractionated with hexane, chloroform and n-butanol. The fractions at different concentrations were subjected to XO inhibitory activity investigation. LC-MS-Q-TOF profiling of the most active fraction was carried out to identify the active compound. Molecular docking was used to investigate the potential inhibitory effect of the active compounds identified through LC-MS-Q-TOF analysis. Results for XO assay demonstrated for n-butanol fraction to display significant inhibitory activity (IC50: 64.84 ± 3.93 μg/mL). However, hexane and chloroform fractions did not show good inhibitory activity. (Z)-Resveratrol-3,4ʹ-β-glucoside and afzelechin-3-O-α-L-rhamnopyranoside were identified in n-butanol fraction through LC-MS-Q-TOF analysis as the possible metabolites responsible for XO inhibitory activity of the n-butanol fraction. Further scientific effort is, however, needed to obtain such bioactive compounds in pure form for complete pharmacological evaluations.
format	Conference or Workshop Item
author	Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Sarian, Murni Nazira
author_facet	Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Sarian, Murni Nazira
author_sort	Ahmed, Qamar Uddin
title	LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves
title_short	LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves
title_full	LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves
title_fullStr	LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves
title_full_unstemmed	LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves
title_sort	lc-ms-q-tof identification of xanthine oxidase inhibitory phytoconstituents from averrhoa bilimbi leaves
publishDate	2018
url	http://irep.iium.edu.my/69002/ http://irep.iium.edu.my/69002/1/Dr%20Qamar%20U.%20Ahmed_PP_CU%2025-26%20Aug%202018.pdf http://irep.iium.edu.my/69002/2/Certificate%20of%20Partcipation.pdf http://irep.iium.edu.my/69002/15/69002-4thPRC2018-1_Abstract%20Final.pdf http://irep.iium.edu.my/69002/16/4IPRC.pdf
first_indexed	2023-09-18T21:37:55Z
last_indexed	2023-09-18T21:37:55Z
_version_	1777412945337122816

LC-MS-Q-TOF identification of xanthine oxidase inhibitory phytoconstituents from Averrhoa bilimbi leaves

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