Factors affecting the in-situ hydrolysis of empty fruit bunches in ionic liquid compatible cellulase system
Utilization of the abundant raw materials in Malaysia as well as surmounting the obstacle of the shortcoming of fossil fuels resources are current concerns of the research. Cellulase development aims to enhance the bioethanol production from lignocellulosic biomass such as empty fruit bunches (EF...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Penerbit Akademia Baru
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/68626/ http://irep.iium.edu.my/68626/ http://irep.iium.edu.my/68626/1/ARFMTSV52_N1_P23_32.pdf http://irep.iium.edu.my/68626/7/68626_Factors%20affecting%20the%20in-situ%20hydrolysis%20of%20empty_scopus.pdf |
| Summary: | Utilization of the abundant raw materials in Malaysia as well as surmounting the
obstacle of the shortcoming of fossil fuels resources are current concerns of the
research. Cellulase development aims to enhance the bioethanol production from
lignocellulosic biomass such as empty fruit bunches (EFB) that resists enzymatic
hydrolysis due to its complex structure. Hence, ionic liquids (ILs) have been
introduced with the ability to disrupt the lignocellulose to be accessible by enzymes
and thus it is expected to achieve a significant improvement by conducting both
treatment and hydrolysis in one step by leaping cellulose regeneration. Palm kernel
cake (PKC) was utilized as the primary media for cellulase production through solidstate
bioconversion (SSB) by Trichoderma reesei. Factors that affect the hydrolysis in
the ionic liquid-cellulase (IL-E) system was investigated using one-factor at a time
(OFAT) and reducing sugar obtained as the response to the study. It was found that
the biomass could be loaded up to 35% (w/v) and agitation has influenced the rate of
reducing sugar production where increasing the speed was associated with higher
concentration. Higher IL concentration in the combined system caused lower
enzymatic activity and hence, less sugar was produced. The results obtained can be
employed for the one-step process in the conversion of EFB into fermentable sugar. |
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