Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water

E. coli O157:H7 is a common microorganism that is responsible for contaminating water after major floods events. Standard methods of pathogen detection cannot be utilized in the field; the need for a portable immunosensor becomes urgent so detection of pathogens can be done at remote post-flood ar...

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Main Authors:	Ali Ahmed, Nasteho, Ramli, Nurul Izzati, Abdul Jalal, Nurfatin Azma, Abd. Wahab, Mohd. Firdaus, Wan Salim, Wan Wardatul Amani
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering International Islamic University Malaysia 2018
Subjects:	QD Chemistry TD169 Environmental protection TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/68448/ http://irep.iium.edu.my/68448/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf

id	iium-68448
recordtype	eprints
spelling	iium-684482019-07-03T07:29:24Z http://irep.iium.edu.my/68448/ Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani QD Chemistry TD169 Environmental protection TP155 Chemical engineering TP248.13 Biotechnology E. coli O157:H7 is a common microorganism that is responsible for contaminating water after major floods events. Standard methods of pathogen detection cannot be utilized in the field; the need for a portable immunosensor becomes urgent so detection of pathogens can be done at remote post-flood areas with no lab and technician access. In this study, the objectives are to analyze E. coli binding to reduced graphene oxide (rGO) electrode surfaces via Linear Sweep Voltammetry (LSV) in phosphate buffered saline (PBS), pH 7.4, and potable tap water, and to correlate voltammetry results with E. coli attachment on electrodes by using FESEM. From the results, LSV in PBS shows sensitivity of 38.7 µA/(cell/ml) in comparison to tap water, with a lower sensitivity of 2.20 µA/(cell/ml). Linear range from 103 to 108 cells/µl was achieved in both solution types. FESEM results show higher attachment of cells on rGO-based electrodes for higher concentration of cells, confirming the lowering of current in LSV measurements owing to increased cell attachment. Kulliyyah of Engineering International Islamic University Malaysia 2018-09-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/68448/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf Ali Ahmed, Nasteho and Ramli, Nurul Izzati and Abdul Jalal, Nurfatin Azma and Abd. Wahab, Mohd. Firdaus and Wan Salim, Wan Wardatul Amani (2018) Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water. In: Proceedings of the International Conference Biotechnology Engineering, ICBioE ‘18, 19-20 September 2018, Gombak, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry TD169 Environmental protection TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	QD Chemistry TD169 Environmental protection TP155 Chemical engineering TP248.13 Biotechnology Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water
description	E. coli O157:H7 is a common microorganism that is responsible for contaminating water after major floods events. Standard methods of pathogen detection cannot be utilized in the field; the need for a portable immunosensor becomes urgent so detection of pathogens can be done at remote post-flood areas with no lab and technician access. In this study, the objectives are to analyze E. coli binding to reduced graphene oxide (rGO) electrode surfaces via Linear Sweep Voltammetry (LSV) in phosphate buffered saline (PBS), pH 7.4, and potable tap water, and to correlate voltammetry results with E. coli attachment on electrodes by using FESEM. From the results, LSV in PBS shows sensitivity of 38.7 µA/(cell/ml) in comparison to tap water, with a lower sensitivity of 2.20 µA/(cell/ml). Linear range from 103 to 108 cells/µl was achieved in both solution types. FESEM results show higher attachment of cells on rGO-based electrodes for higher concentration of cells, confirming the lowering of current in LSV measurements owing to increased cell attachment.
format	Conference or Workshop Item
author	Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani
author_facet	Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani
author_sort	Ali Ahmed, Nasteho
title	Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water
title_short	Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water
title_full	Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water
title_fullStr	Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water
title_full_unstemmed	Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water
title_sort	quantifying e. coli o157:h7 via linear sweep voltammetry in phosphate buffered saline and tap water
publisher	Kulliyyah of Engineering International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/68448/ http://irep.iium.edu.my/68448/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf
first_indexed	2023-09-18T21:37:09Z
last_indexed	2023-09-18T21:37:09Z
_version_	1777412897208532992

Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water

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