Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method

Traditionally Trachyspermum ammi has been used for the treatment of immune disease such as rheumatism. The aim and objective of this study was to find the immunostimulant effect of three different extracts of T. ammi seeds. To assess the immunomodulatory effect, delayed-type hypersensitivity (DTH)...

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Main Authors:	Siddiqui, Mohammad Jamshed Ahmad, Aslam, Adeel, Khan, Tanveer
Format:	Article
Language:	English English English
Published:	Wolters Kluwer - Medknow 2019
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/68347/ http://irep.iium.edu.my/68347/ http://irep.iium.edu.my/68347/ http://irep.iium.edu.my/68347/1/68347_Comparison%20and%20evaluation%20of%20different%20seed.pdf http://irep.iium.edu.my/68347/2/68347_Comparison%20and%20evaluation%20of%20different%20seed_SCOPUS.pdf http://irep.iium.edu.my/68347/3/68347_Comparison%20and%20evaluation%20of%20different%20seed_WOS.pdf

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spelling	iium-683472019-03-11T07:20:44Z http://irep.iium.edu.my/68347/ Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method Siddiqui, Mohammad Jamshed Ahmad Aslam, Adeel Khan, Tanveer QD Chemistry Traditionally Trachyspermum ammi has been used for the treatment of immune disease such as rheumatism. The aim and objective of this study was to find the immunostimulant effect of three different extracts of T. ammi seeds. To assess the immunomodulatory effect, delayed-type hypersensitivity (DTH) assay method was used and by the DTH assay, the effect of T. ammi on the skin thickness of rats was estimated. Seeds of T. ammi were extracted using three different solvents—n-hexane, chloroform, and methanol—by using Soxhlet apparatus. To find the exact dose for administration, acute toxicity test was performed using crude methanolic extract at a dose of 400, 800, 1600, and 3200 mg/kg. After acute toxicity test, 500 mg/kg dose was determined as safe for therapeutic effect and immunomodulatory effect was evaluated at this dose. Dose of 500 mg/kg was administered to Wistar rats daily for 14 days and skin thickness of rats was measured at 24, 48, and 72 h. Results were obtained from six groups of rats, which were positive control group, negative control group, and the groups receiving the test drugs. Standard drug was the combination of sodium selenite, vitamin E, and sodium chloride and it showed more positive results as compared to that of test drug. Furthermore, among the three extracts, methanol extract showed more effectiveness on skin thickness. There was a meaningful difference in the skin thickness of rats when the skin thickness was measured on the 2nd and 14th day of activity. Wolters Kluwer - Medknow 2019-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68347/1/68347_Comparison%20and%20evaluation%20of%20different%20seed.pdf application/pdf en http://irep.iium.edu.my/68347/2/68347_Comparison%20and%20evaluation%20of%20different%20seed_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/68347/3/68347_Comparison%20and%20evaluation%20of%20different%20seed_WOS.pdf Siddiqui, Mohammad Jamshed Ahmad and Aslam, Adeel and Khan, Tanveer (2019) Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method. Journal of Pharmacy and Bioallied Sciences, 11 (1 (Jan-March)). pp. 49-59. ISSN 0976-4879 http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2019;volume=11;issue=1;spage=43;epage=48;aulast=Siddiqui;type=0 10.4103/JPBS.JPBS_174_18
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QD Chemistry
spellingShingle	QD Chemistry Siddiqui, Mohammad Jamshed Ahmad Aslam, Adeel Khan, Tanveer Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method
description	Traditionally Trachyspermum ammi has been used for the treatment of immune disease such as rheumatism. The aim and objective of this study was to find the immunostimulant effect of three different extracts of T. ammi seeds. To assess the immunomodulatory effect, delayed-type hypersensitivity (DTH) assay method was used and by the DTH assay, the effect of T. ammi on the skin thickness of rats was estimated. Seeds of T. ammi were extracted using three different solvents—n-hexane, chloroform, and methanol—by using Soxhlet apparatus. To find the exact dose for administration, acute toxicity test was performed using crude methanolic extract at a dose of 400, 800, 1600, and 3200 mg/kg. After acute toxicity test, 500 mg/kg dose was determined as safe for therapeutic effect and immunomodulatory effect was evaluated at this dose. Dose of 500 mg/kg was administered to Wistar rats daily for 14 days and skin thickness of rats was measured at 24, 48, and 72 h. Results were obtained from six groups of rats, which were positive control group, negative control group, and the groups receiving the test drugs. Standard drug was the combination of sodium selenite, vitamin E, and sodium chloride and it showed more positive results as compared to that of test drug. Furthermore, among the three extracts, methanol extract showed more effectiveness on skin thickness. There was a meaningful difference in the skin thickness of rats when the skin thickness was measured on the 2nd and 14th day of activity.
format	Article
author	Siddiqui, Mohammad Jamshed Ahmad Aslam, Adeel Khan, Tanveer
author_facet	Siddiqui, Mohammad Jamshed Ahmad Aslam, Adeel Khan, Tanveer
author_sort	Siddiqui, Mohammad Jamshed Ahmad
title	Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method
title_short	Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method
title_full	Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method
title_fullStr	Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method
title_full_unstemmed	Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method
title_sort	comparison and evaluation of different seed extracts of trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method
publisher	Wolters Kluwer - Medknow
publishDate	2019
url	http://irep.iium.edu.my/68347/ http://irep.iium.edu.my/68347/ http://irep.iium.edu.my/68347/ http://irep.iium.edu.my/68347/1/68347_Comparison%20and%20evaluation%20of%20different%20seed.pdf http://irep.iium.edu.my/68347/2/68347_Comparison%20and%20evaluation%20of%20different%20seed_SCOPUS.pdf http://irep.iium.edu.my/68347/3/68347_Comparison%20and%20evaluation%20of%20different%20seed_WOS.pdf
first_indexed	2023-09-18T21:37:00Z
last_indexed	2023-09-18T21:37:00Z
_version_	1777412887382327296

Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method

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