Biosynthesis of nanoparticles using recombinant bromelain

Green chemical method was applied to synthesize nanoparticles using recombinant bromelain. Among the numerous applications of recombinant bromelain, there is still no research on nanoparticles synthesis which encourages its utilization in this study. Four chemicals which are copper (II) chloride...

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Main Authors:	Nasruddin, Arina, Amid, Azura, Sulaiman, Sarina, Othman, Muhd. Ezza Faiez
Format:	Article
Language:	English
Published:	IIUM Press, International Islamic University Malaysia 2018
Subjects:	TP368 Food processing and manufacture
Online Access:	http://irep.iium.edu.my/68285/ http://irep.iium.edu.my/68285/ http://irep.iium.edu.my/68285/1/68285_Biosynthesis%20of%20nanoparticles.pdf

id	iium-68285
recordtype	eprints
spelling	iium-682852019-07-11T02:11:04Z http://irep.iium.edu.my/68285/ Biosynthesis of nanoparticles using recombinant bromelain Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez TP368 Food processing and manufacture Green chemical method was applied to synthesize nanoparticles using recombinant bromelain. Among the numerous applications of recombinant bromelain, there is still no research on nanoparticles synthesis which encourages its utilization in this study. Four chemicals which are copper (II) chloride dihydrate (CuCl2.2H2O), cerium nitrate hexahydrate (Ce(NO3)3.6H2O), sodium selenite (Na2SeO3), and iron (III) chloride hexahydrate (FeCl3.6H2O) were selected to be screened for the suitability in nanoparticles biosynthesis by recombinant bromelain. The nanoparticles formed were characterized by using UV-visible absorption spectra. The biosynthesis process then was optimized by varying the centrifugation speed, temperature, and time to get the maximum absorption and weight of nanoparticles through central composite design (CCD) tool. Only CuCl2.2H2O showed a positive result for the screening process which was represented by the formation of colloidal solution and a maximum absorption at 580 nm. Thus, optimization was carried out for this chemical. Based on the optimization model, maximum absorption and weight were predicted at 67.5°C, 2 hrs, and 9,600 rpm. These optimal conditions were validated by repeating the biosynthesis process. The absorption and weight of the nanoparticles depended on the reaction of the chemical with recombinant bromelain. 3D plots showed that the optimal condition for high responses mostly depends on temperature and time. IIUM Press, International Islamic University Malaysia 2018-11-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68285/1/68285_Biosynthesis%20of%20nanoparticles.pdf Nasruddin, Arina and Amid, Azura and Sulaiman, Sarina and Othman, Muhd. Ezza Faiez (2018) Biosynthesis of nanoparticles using recombinant bromelain. Biological and Natural Resources Engineering Journal, 1 (1). pp. 14-25. E-ISSN 2637-0719 http://journals.iium.edu.my/bnrej/index.php/bnrej/issue/current
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP368 Food processing and manufacture
spellingShingle	TP368 Food processing and manufacture Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez Biosynthesis of nanoparticles using recombinant bromelain
description	Green chemical method was applied to synthesize nanoparticles using recombinant bromelain. Among the numerous applications of recombinant bromelain, there is still no research on nanoparticles synthesis which encourages its utilization in this study. Four chemicals which are copper (II) chloride dihydrate (CuCl2.2H2O), cerium nitrate hexahydrate (Ce(NO3)3.6H2O), sodium selenite (Na2SeO3), and iron (III) chloride hexahydrate (FeCl3.6H2O) were selected to be screened for the suitability in nanoparticles biosynthesis by recombinant bromelain. The nanoparticles formed were characterized by using UV-visible absorption spectra. The biosynthesis process then was optimized by varying the centrifugation speed, temperature, and time to get the maximum absorption and weight of nanoparticles through central composite design (CCD) tool. Only CuCl2.2H2O showed a positive result for the screening process which was represented by the formation of colloidal solution and a maximum absorption at 580 nm. Thus, optimization was carried out for this chemical. Based on the optimization model, maximum absorption and weight were predicted at 67.5°C, 2 hrs, and 9,600 rpm. These optimal conditions were validated by repeating the biosynthesis process. The absorption and weight of the nanoparticles depended on the reaction of the chemical with recombinant bromelain. 3D plots showed that the optimal condition for high responses mostly depends on temperature and time.
format	Article
author	Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez
author_facet	Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez
author_sort	Nasruddin, Arina
title	Biosynthesis of nanoparticles using recombinant bromelain
title_short	Biosynthesis of nanoparticles using recombinant bromelain
title_full	Biosynthesis of nanoparticles using recombinant bromelain
title_fullStr	Biosynthesis of nanoparticles using recombinant bromelain
title_full_unstemmed	Biosynthesis of nanoparticles using recombinant bromelain
title_sort	biosynthesis of nanoparticles using recombinant bromelain
publisher	IIUM Press, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/68285/ http://irep.iium.edu.my/68285/ http://irep.iium.edu.my/68285/1/68285_Biosynthesis%20of%20nanoparticles.pdf
first_indexed	2023-09-18T21:36:54Z
last_indexed	2023-09-18T21:36:54Z
_version_	1777412881275420672

Biosynthesis of nanoparticles using recombinant bromelain

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