Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King

This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methan...

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Main Authors:	Obaid, Ahmed Kareem, Syed Abdul Azziz, Saripah Salbiah, Mhd Bakri, Yuhanis, Nafiah, Mohd Azlan, Awang, Khalijah, Abdulla Aowda, Saadon, Mohd Hassan, Norazian, Hashim, Yumi Zuhanis Has-Yun, Abdullah, Fauziah
Format:	Article
Language:	English English
Published:	PharmaInfo Publications 2018
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/68245/ http://irep.iium.edu.my/68245/ http://irep.iium.edu.my/68245/1/jpsr10111807.pdf http://irep.iium.edu.my/68245/7/68245_Antioxidative%20and%20cytotoxic%20activities%20of%20crude%20and%20isolated%20compounds_scopus.pdf

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spelling	iium-682452019-01-09T01:01:10Z http://irep.iium.edu.my/68245/ Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King Obaid, Ahmed Kareem Syed Abdul Azziz, Saripah Salbiah Mhd Bakri, Yuhanis Nafiah, Mohd Azlan Awang, Khalijah Abdulla Aowda, Saadon Mohd Hassan, Norazian Hashim, Yumi Zuhanis Has-Yun Abdullah, Fauziah QD Chemistry RS Pharmacy and materia medica This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methanol. Phytochemical investigation of the crude extracts has led to the isolation of four oxoaporphine alkaloids (O-methylmoschatoline, atherospermidine, lysicamine, and liriodenine) and two steroids (stigmasterol and lupeol). The chemical structure of the compounds was elucidated using 1H-NMR, 13C-NMR, 2D-NMR, and mass spectral analysis. All the crude extracts and isolated compounds were tested for their in vitro DPPH radicals scavenging activity and cytotoxicity against MCF-7 breast cancer cells. Methanol extract showed the strongest antioxidant activity with percentage inhibition at 500μg/mL of 89.52±1.81 (IC50, 128.22± 6.73 μg/ml) in comparison with DCM (32.45±8.56), atherospermidine (20.17±9.74) and liriodenine (10.73±0.45), while other extracts were inactive. All the isolated compounds showed cytotoxic against MCF-7 cells, at IC50 values in the range of 42-89 μg/ml. Based on thorough literature review, all compounds identified in this study were isolated for the first time from this plant. In particular, lupeol is reported for the first time in the genus Polyalthia and possessed the most potent cytotoxic effect compared to other isolated compounds. PharmaInfo Publications 2018-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68245/1/jpsr10111807.pdf application/pdf en http://irep.iium.edu.my/68245/7/68245_Antioxidative%20and%20cytotoxic%20activities%20of%20crude%20and%20isolated%20compounds_scopus.pdf Obaid, Ahmed Kareem and Syed Abdul Azziz, Saripah Salbiah and Mhd Bakri, Yuhanis and Nafiah, Mohd Azlan and Awang, Khalijah and Abdulla Aowda, Saadon and Mohd Hassan, Norazian and Hashim, Yumi Zuhanis Has-Yun and Abdullah, Fauziah (2018) Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King. Journal of Pharmaceutical Sciences and Research, 10 (11). pp. 2718-2721. ISSN 0975-1459 https://www.jpsr.pharmainfo.in/Documents/Volumes/vol10Issue11/jpsr10111807.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Obaid, Ahmed Kareem Syed Abdul Azziz, Saripah Salbiah Mhd Bakri, Yuhanis Nafiah, Mohd Azlan Awang, Khalijah Abdulla Aowda, Saadon Mohd Hassan, Norazian Hashim, Yumi Zuhanis Has-Yun Abdullah, Fauziah Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King
description	This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methanol. Phytochemical investigation of the crude extracts has led to the isolation of four oxoaporphine alkaloids (O-methylmoschatoline, atherospermidine, lysicamine, and liriodenine) and two steroids (stigmasterol and lupeol). The chemical structure of the compounds was elucidated using 1H-NMR, 13C-NMR, 2D-NMR, and mass spectral analysis. All the crude extracts and isolated compounds were tested for their in vitro DPPH radicals scavenging activity and cytotoxicity against MCF-7 breast cancer cells. Methanol extract showed the strongest antioxidant activity with percentage inhibition at 500μg/mL of 89.52±1.81 (IC50, 128.22± 6.73 μg/ml) in comparison with DCM (32.45±8.56), atherospermidine (20.17±9.74) and liriodenine (10.73±0.45), while other extracts were inactive. All the isolated compounds showed cytotoxic against MCF-7 cells, at IC50 values in the range of 42-89 μg/ml. Based on thorough literature review, all compounds identified in this study were isolated for the first time from this plant. In particular, lupeol is reported for the first time in the genus Polyalthia and possessed the most potent cytotoxic effect compared to other isolated compounds.
format	Article
author	Obaid, Ahmed Kareem Syed Abdul Azziz, Saripah Salbiah Mhd Bakri, Yuhanis Nafiah, Mohd Azlan Awang, Khalijah Abdulla Aowda, Saadon Mohd Hassan, Norazian Hashim, Yumi Zuhanis Has-Yun Abdullah, Fauziah
author_facet	Obaid, Ahmed Kareem Syed Abdul Azziz, Saripah Salbiah Mhd Bakri, Yuhanis Nafiah, Mohd Azlan Awang, Khalijah Abdulla Aowda, Saadon Mohd Hassan, Norazian Hashim, Yumi Zuhanis Has-Yun Abdullah, Fauziah
author_sort	Obaid, Ahmed Kareem
title	Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King
title_short	Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King
title_full	Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King
title_fullStr	Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King
title_full_unstemmed	Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King
title_sort	antioxidative and cytotoxic activities of crude and isolated compounds of polyalthia lateriflora (bl.) king
publisher	PharmaInfo Publications
publishDate	2018
url	http://irep.iium.edu.my/68245/ http://irep.iium.edu.my/68245/ http://irep.iium.edu.my/68245/1/jpsr10111807.pdf http://irep.iium.edu.my/68245/7/68245_Antioxidative%20and%20cytotoxic%20activities%20of%20crude%20and%20isolated%20compounds_scopus.pdf
first_indexed	2023-09-18T21:36:51Z
last_indexed	2023-09-18T21:36:51Z
_version_	1777412878572191744

Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King

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