Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract

Background: In practice, either the herbs themselves or the crude extracts are used for traditional remedies but not the isolated molecules. Therefore, the importance of toxicity and clot lysis study of the crude extract is beyond description. Objective: The aim of our study was to find out th...

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Main Authors:	Azad, A.K., Sarker, Md. Zaidul Islam, Helaluddin, Abul Bashar Mohammed, Alaama, Mohamed, Abdullah, J.
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/68159/ http://irep.iium.edu.my/68159/1/IPRC-2018-1.pdf

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recordtype	eprints
spelling	iium-681592019-07-05T00:36:47Z http://irep.iium.edu.my/68159/ Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract Azad, A.K. Sarker, Md. Zaidul Islam Helaluddin, Abul Bashar Mohammed Alaama, Mohamed Abdullah, J. QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Background: In practice, either the herbs themselves or the crude extracts are used for traditional remedies but not the isolated molecules. Therefore, the importance of toxicity and clot lysis study of the crude extract is beyond description. Objective: The aim of our study was to find out the preliminary cytotoxic and thrombolytic effect of the seven selected medicinal plant leaves extract. Methods: Cold maceration was performed using ethanol for extraction purposes. The cytotoxicity study was done by using brine shrimp nauplii (Artemia salina) and MTT assays by using MCF-7 cell line. Result: In the cytotoxic activity, out of the seven leaves extract three of them exhibited stronger brine shrimp lethality with 4 TH PHARMACEUTICAL RESEARCH CONFERENCE 2018 Challenging The Inquisitive Minds: Frontier Of The Future 24 – 26 August 2018 \| Cyberjaya, Malaysia LC50 122.548 (Uncaria acida), 170.861 (Leea indica) and 175.469 (Piper porphyrophyllum) μg /mL respectively, and on MCF-7 cell line, they also exhibited moderate cytotoxic activity with different concentration of the extract of the same plant leaves such as, Uncaria acida (72.31, 56.22, 38.12 and 9.24%), Leea indica (67.31, 58.22, 43.12 and 15.24%), Piper porphyrophyllum (65.88, 48.12, 40.12 and 21.34%). In thrombolytic assessments, all the leaves extract showed moderate (considering > 20% moderate; *p < 0.01; p < 0.05) clot lysis activity, but among the extracts, Uncaria cordata (27.36 ± 0.10%) showed the highest and Stachytarpheta indica the lowest (6.14 ± 0.20%) percent clot lysis as compared with the standard streptokinase (65.15 ± 0.16%). Conclusion: This study was conducted to support the folkloric use of seven medicinal plant leaves to improve cancer or myocardial infarction. Although, it is a preliminary screening but it will be targeted to the scientific community to put more attention and to do an extensive and molecular level study in future. 2018 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/68159/1/IPRC-2018-1.pdf Azad, A.K. and Sarker, Md. Zaidul Islam and Helaluddin, Abul Bashar Mohammed and Alaama, Mohamed and Abdullah, J. (2018) Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract. In: 4th International Pharmaceutical Research Conference 2018, 24-26 August 2018, Cyberjaya. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Azad, A.K. Sarker, Md. Zaidul Islam Helaluddin, Abul Bashar Mohammed Alaama, Mohamed Abdullah, J. Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
description	Background: In practice, either the herbs themselves or the crude extracts are used for traditional remedies but not the isolated molecules. Therefore, the importance of toxicity and clot lysis study of the crude extract is beyond description. Objective: The aim of our study was to find out the preliminary cytotoxic and thrombolytic effect of the seven selected medicinal plant leaves extract. Methods: Cold maceration was performed using ethanol for extraction purposes. The cytotoxicity study was done by using brine shrimp nauplii (Artemia salina) and MTT assays by using MCF-7 cell line. Result: In the cytotoxic activity, out of the seven leaves extract three of them exhibited stronger brine shrimp lethality with 4 TH PHARMACEUTICAL RESEARCH CONFERENCE 2018 Challenging The Inquisitive Minds: Frontier Of The Future 24 – 26 August 2018 \| Cyberjaya, Malaysia LC50 122.548 (Uncaria acida), 170.861 (Leea indica) and 175.469 (Piper porphyrophyllum) μg /mL respectively, and on MCF-7 cell line, they also exhibited moderate cytotoxic activity with different concentration of the extract of the same plant leaves such as, Uncaria acida (72.31, 56.22, 38.12 and 9.24%), Leea indica (67.31, 58.22, 43.12 and 15.24%), Piper porphyrophyllum (65.88, 48.12, 40.12 and 21.34%). In thrombolytic assessments, all the leaves extract showed moderate (considering > 20% moderate; *p < 0.01; p < 0.05) clot lysis activity, but among the extracts, Uncaria cordata (27.36 ± 0.10%) showed the highest and Stachytarpheta indica the lowest (6.14 ± 0.20%) percent clot lysis as compared with the standard streptokinase (65.15 ± 0.16%). Conclusion: This study was conducted to support the folkloric use of seven medicinal plant leaves to improve cancer or myocardial infarction. Although, it is a preliminary screening but it will be targeted to the scientific community to put more attention and to do an extensive and molecular level study in future.
format	Conference or Workshop Item
author	Azad, A.K. Sarker, Md. Zaidul Islam Helaluddin, Abul Bashar Mohammed Alaama, Mohamed Abdullah, J.
author_facet	Azad, A.K. Sarker, Md. Zaidul Islam Helaluddin, Abul Bashar Mohammed Alaama, Mohamed Abdullah, J.
author_sort	Azad, A.K.
title	Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
title_short	Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
title_full	Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
title_fullStr	Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
title_full_unstemmed	Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
title_sort	cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
publishDate	2018
url	http://irep.iium.edu.my/68159/ http://irep.iium.edu.my/68159/1/IPRC-2018-1.pdf
first_indexed	2023-09-18T21:36:44Z
last_indexed	2023-09-18T21:36:44Z
_version_	1777412870809583616

Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract

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