Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology

Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response S...

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Main Authors:	Ulhiza, Tami Astie, Mohamad Puad, Noor Illi, Azmi, Azlin Suhaida
Format:	Article
Language:	English English English
Published:	Elsevier 2018
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/1/Published%20article.pdf http://irep.iium.edu.my/67867/13/67867_Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20from%20sago%20wastewater_scopus.pdf http://irep.iium.edu.my/67867/18/67867%20Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20WOS.pdf

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spelling	iium-678672019-01-27T06:46:43Z http://irep.iium.edu.my/67867/ Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida TP248.13 Biotechnology Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response Surface Methodology (RSM) was employed to find the optimum conditions. From preliminary optimization, it was found that the most significant factors were yeast extract, temperature, and inoculum size. According to Face Centered Central Composite Design (FCCCD), the maximum hydrogen concentration and yield were 630.67 μmol/L and 7.42 mmol H2/mol glucose, respectively, which is obtained from the sample supplemented with 4.8 g/L yeast extract concentration, 5% inoculum, and incubated at the temperature of 31 °C. Cumulative hydrogen production curve fitted by the modified Gompertz equation suggested that Hmax, Rmax, and λ from this study were 15.10 mL, 2.18 mL/h, and 9.84 h, respectively. Elsevier 2018-10-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67867/1/Published%20article.pdf application/pdf en http://irep.iium.edu.my/67867/13/67867_Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20from%20sago%20wastewater_scopus.pdf application/pdf en http://irep.iium.edu.my/67867/18/67867%20Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20WOS.pdf Ulhiza, Tami Astie and Mohamad Puad, Noor Illi and Azmi, Azlin Suhaida (2018) Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology. International Journal of Hydrogen Energy, 43 (49). pp. 22148-22158. ISSN 0360-3199 https://www.sciencedirect.com/science/article/pii/S0360319918332348?dgcid=author 10.1016/j.ijhydene.2018.10.057
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
description	Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response Surface Methodology (RSM) was employed to find the optimum conditions. From preliminary optimization, it was found that the most significant factors were yeast extract, temperature, and inoculum size. According to Face Centered Central Composite Design (FCCCD), the maximum hydrogen concentration and yield were 630.67 μmol/L and 7.42 mmol H2/mol glucose, respectively, which is obtained from the sample supplemented with 4.8 g/L yeast extract concentration, 5% inoculum, and incubated at the temperature of 31 °C. Cumulative hydrogen production curve fitted by the modified Gompertz equation suggested that Hmax, Rmax, and λ from this study were 15.10 mL, 2.18 mL/h, and 9.84 h, respectively.
format	Article
author	Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida
author_facet	Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida
author_sort	Ulhiza, Tami Astie
title	Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
title_short	Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
title_full	Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
title_fullStr	Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
title_full_unstemmed	Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
title_sort	optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
publisher	Elsevier
publishDate	2018
url	http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/1/Published%20article.pdf http://irep.iium.edu.my/67867/13/67867_Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20from%20sago%20wastewater_scopus.pdf http://irep.iium.edu.my/67867/18/67867%20Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20WOS.pdf
first_indexed	2023-09-18T21:36:22Z
last_indexed	2023-09-18T21:36:22Z
_version_	1777412847422144512

Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology

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