Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia

Actinomycetes are biotechnologically important for their unparalleled capability to produce various bioactive secondary metabolites. The diversity and antimicrobial capabilities were investigated for actinomycetes, which were previously isolated from mangrove sediment of the Pahang mangrove forest....

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Main Authors:	Zainal Abidin, Zaima Azira, Khan Chowdhury, Ahmed Jalal, Abdul Malek, Nurfathiah, Zainuddin, Zarina
Format:	Article
Language:	English English English
Published:	Coastal Education & Research Foundation Inc. Florida 2018
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/67808/ http://irep.iium.edu.my/67808/ http://irep.iium.edu.my/67808/ http://irep.iium.edu.my/67808/7/67808%20Diversity%2C%20Antimicrobial%20Capabilities.pdf http://irep.iium.edu.my/67808/8/67808%20Diversity%2C%20Antimicrobial%20Capabilities%20SCOPUS.pdf http://irep.iium.edu.my/67808/19/97808_Diversity%2C%20antimicrobial%20capabilities_WOS.pdf

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spelling	iium-678082019-01-30T03:00:48Z http://irep.iium.edu.my/67808/ Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia Zainal Abidin, Zaima Azira Khan Chowdhury, Ahmed Jalal Abdul Malek, Nurfathiah Zainuddin, Zarina QR Microbiology Actinomycetes are biotechnologically important for their unparalleled capability to produce various bioactive secondary metabolites. The diversity and antimicrobial capabilities were investigated for actinomycetes, which were previously isolated from mangrove sediment of the Pahang mangrove forest. The biosynthetic potential using type I polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS) genes and the morphological characteristics of actinomycetes were also determined. Representative isolates were selected from five sampling locations in the Kuantan mangrove forest. Antimicrobial activities of crude ethyl acetate extract of these actinomycetes against five test microorganisms—Bacillus subtilis, Candida albicans, Escherichia coli, Serratia marcescens, and Staphylococcus aureus—were evaluated using the disc diffusion method. Actinomycetes were identified using the 16S rRNA gene sequence, and culture characteristics of selected actinomycetes were determined by inoculating the strains onto a set of agar media, namely, starch–yeast extract agar, inorganic salt–starch agar, starch–casein agar, and nutrient agar. Screening of the biosynthetic potential of these actinomycetes was achieved through polymerase chain reaction amplification of PKS-I and NRPS genes. Most isolates exhibited moderate to good antimicrobial activities toward B. subtilis and S. aureus, and a few inhibited C. albicans and E. coli. Streptomyces sp. K1-01 and Streptomyces sp. K2-03 showed antimicrobial activity against all test organisms, suggesting a broad-spectrum nature of the compounds they produced. Mangrove actinomycetes enumerated in this study demonstrated a high level of diversity, and they were distributed among nine genera. Streptomyces and Micromonospora were the most predominant genera observed,alongside Micrococcus, Gordonia, Nocardia, Dietzia, Pseudonocardia, Saccharopolyspora, and Verrucosispora. However,there is no direct correlation between antimicrobial capability and presence of PKS-I and NRPS in actinomycetes.Cultural characterization of selected actinomycetes revealed two interesting isolates Micromonospora sp. K3-13,producing blue diffusible pigment, and Streptomyces sp. K2-03, producing purple diffusible pigment. This study demonstrated the potential of mangrove sediment as a new resource for highly diverse actinomycetes with biosynthetic capabilities. Detection of PKS-I and NRPS in these actinomycetes helps to establish a focus group that can be applied for future study of the natural product, particularly in antibiotic production. Coastal Education & Research Foundation Inc. Florida 2018-11-17 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67808/7/67808%20Diversity%2C%20Antimicrobial%20Capabilities.pdf application/pdf en http://irep.iium.edu.my/67808/8/67808%20Diversity%2C%20Antimicrobial%20Capabilities%20SCOPUS.pdf application/pdf en http://irep.iium.edu.my/67808/19/97808_Diversity%2C%20antimicrobial%20capabilities_WOS.pdf Zainal Abidin, Zaima Azira and Khan Chowdhury, Ahmed Jalal and Abdul Malek, Nurfathiah and Zainuddin, Zarina (2018) Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia. Journal of Coastal Research, Special Issue 82 Fall. pp. 174-179. ISSN 0749-0208 E-ISSN 0749-0208 http://www.jcronline.org/doi/pdf/10.2112/SI82-025.1 10.2112/SI82-025.1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Zainal Abidin, Zaima Azira Khan Chowdhury, Ahmed Jalal Abdul Malek, Nurfathiah Zainuddin, Zarina Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia
description	Actinomycetes are biotechnologically important for their unparalleled capability to produce various bioactive secondary metabolites. The diversity and antimicrobial capabilities were investigated for actinomycetes, which were previously isolated from mangrove sediment of the Pahang mangrove forest. The biosynthetic potential using type I polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS) genes and the morphological characteristics of actinomycetes were also determined. Representative isolates were selected from five sampling locations in the Kuantan mangrove forest. Antimicrobial activities of crude ethyl acetate extract of these actinomycetes against five test microorganisms—Bacillus subtilis, Candida albicans, Escherichia coli, Serratia marcescens, and Staphylococcus aureus—were evaluated using the disc diffusion method. Actinomycetes were identified using the 16S rRNA gene sequence, and culture characteristics of selected actinomycetes were determined by inoculating the strains onto a set of agar media, namely, starch–yeast extract agar, inorganic salt–starch agar, starch–casein agar, and nutrient agar. Screening of the biosynthetic potential of these actinomycetes was achieved through polymerase chain reaction amplification of PKS-I and NRPS genes. Most isolates exhibited moderate to good antimicrobial activities toward B. subtilis and S. aureus, and a few inhibited C. albicans and E. coli. Streptomyces sp. K1-01 and Streptomyces sp. K2-03 showed antimicrobial activity against all test organisms, suggesting a broad-spectrum nature of the compounds they produced. Mangrove actinomycetes enumerated in this study demonstrated a high level of diversity, and they were distributed among nine genera. Streptomyces and Micromonospora were the most predominant genera observed,alongside Micrococcus, Gordonia, Nocardia, Dietzia, Pseudonocardia, Saccharopolyspora, and Verrucosispora. However,there is no direct correlation between antimicrobial capability and presence of PKS-I and NRPS in actinomycetes.Cultural characterization of selected actinomycetes revealed two interesting isolates Micromonospora sp. K3-13,producing blue diffusible pigment, and Streptomyces sp. K2-03, producing purple diffusible pigment. This study demonstrated the potential of mangrove sediment as a new resource for highly diverse actinomycetes with biosynthetic capabilities. Detection of PKS-I and NRPS in these actinomycetes helps to establish a focus group that can be applied for future study of the natural product, particularly in antibiotic production.
format	Article
author	Zainal Abidin, Zaima Azira Khan Chowdhury, Ahmed Jalal Abdul Malek, Nurfathiah Zainuddin, Zarina
author_facet	Zainal Abidin, Zaima Azira Khan Chowdhury, Ahmed Jalal Abdul Malek, Nurfathiah Zainuddin, Zarina
author_sort	Zainal Abidin, Zaima Azira
title	Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia
title_short	Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia
title_full	Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia
title_fullStr	Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia
title_full_unstemmed	Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia
title_sort	diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in pahang, malaysia
publisher	Coastal Education & Research Foundation Inc. Florida
publishDate	2018
url	http://irep.iium.edu.my/67808/ http://irep.iium.edu.my/67808/ http://irep.iium.edu.my/67808/ http://irep.iium.edu.my/67808/7/67808%20Diversity%2C%20Antimicrobial%20Capabilities.pdf http://irep.iium.edu.my/67808/8/67808%20Diversity%2C%20Antimicrobial%20Capabilities%20SCOPUS.pdf http://irep.iium.edu.my/67808/19/97808_Diversity%2C%20antimicrobial%20capabilities_WOS.pdf
first_indexed	2023-09-18T21:36:16Z
last_indexed	2023-09-18T21:36:16Z
_version_	1777412841584721920

Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia

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