Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene

Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was c...

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Main Authors:	Kamarudin, Kamarul Rahim, Mohamed Rehan, Maryam, Mohamed Rehan, Aisyah
Format:	Article
Language:	English English
Published:	UPM Press 2018
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/67774/ http://irep.iium.edu.my/67774/ http://irep.iium.edu.my/67774/1/2018%20JTAS%20Kamarudin%20et%20al.%2012S%20Processed%20Sea%20Cucumber.pdf http://irep.iium.edu.my/67774/7/67774_Species%20identification%20and%20molecular_scopus.pdf

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spelling	iium-677742018-12-19T03:58:59Z http://irep.iium.edu.my/67774/ Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah QL Zoology Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was conducted to determine the species identities of processed sea cucumbers from selected Malaysian markets using non-protein-coding 12S mitochondrial rRNA gene. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 81 ingroup sequences representing 63 processed sea cucumber specimens, 13 fresh and processed reference samples for species identification, and five fresh additional specimens from Teluk Nipah Beach, Pangkor Archipelago and Manukan Island, Sabah suggested the presence of three main clusters i.e. a gamat family cluster consisting of family Stichopodidae (Stichopus horrens, Stichopus vastus, and Thelenota anax) and two clusters of timun laut family comprising family Holothuriidae (Holothuria (Mertensiothuria) leucospilota, Holothuria (Metriatyla)scabra, Holothuria (Metriatyla) lessoni, Holothuria (Halodeima) atra, and Holothuria (Halodeima) edulis) and family Caudinidae (Acaudina molpadioides). The outcomes of this study also highlighted the availability of 40 new 12S mitochondrial rRNA gene sequences deposited in the GenBank that can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets. UPM Press 2018-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67774/1/2018%20JTAS%20Kamarudin%20et%20al.%2012S%20Processed%20Sea%20Cucumber.pdf application/pdf en http://irep.iium.edu.my/67774/7/67774_Species%20identification%20and%20molecular_scopus.pdf Kamarudin, Kamarul Rahim and Mohamed Rehan, Maryam and Mohamed Rehan, Aisyah (2018) Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene. Pertanika Journal of Tropical Agricultural Science, 41 (4). pp. 1833-1851. ISSN 1511-3701 E-ISSN 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2041%20(4)%20Nov.%202018/19%20JTAS-1404-2018.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QL Zoology
spellingShingle	QL Zoology Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene
description	Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was conducted to determine the species identities of processed sea cucumbers from selected Malaysian markets using non-protein-coding 12S mitochondrial rRNA gene. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 81 ingroup sequences representing 63 processed sea cucumber specimens, 13 fresh and processed reference samples for species identification, and five fresh additional specimens from Teluk Nipah Beach, Pangkor Archipelago and Manukan Island, Sabah suggested the presence of three main clusters i.e. a gamat family cluster consisting of family Stichopodidae (Stichopus horrens, Stichopus vastus, and Thelenota anax) and two clusters of timun laut family comprising family Holothuriidae (Holothuria (Mertensiothuria) leucospilota, Holothuria (Metriatyla)scabra, Holothuria (Metriatyla) lessoni, Holothuria (Halodeima) atra, and Holothuria (Halodeima) edulis) and family Caudinidae (Acaudina molpadioides). The outcomes of this study also highlighted the availability of 40 new 12S mitochondrial rRNA gene sequences deposited in the GenBank that can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets.
format	Article
author	Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah
author_facet	Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah
author_sort	Kamarudin, Kamarul Rahim
title	Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene
title_short	Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene
title_full	Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene
title_fullStr	Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene
title_full_unstemmed	Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene
title_sort	species identification and molecular phylogenetics of processed sea cucumbers from malaysian market based on 12s mitochondrial rrna gene
publisher	UPM Press
publishDate	2018
url	http://irep.iium.edu.my/67774/ http://irep.iium.edu.my/67774/ http://irep.iium.edu.my/67774/1/2018%20JTAS%20Kamarudin%20et%20al.%2012S%20Processed%20Sea%20Cucumber.pdf http://irep.iium.edu.my/67774/7/67774_Species%20identification%20and%20molecular_scopus.pdf
first_indexed	2023-09-18T21:36:13Z
last_indexed	2023-09-18T21:36:13Z
_version_	1777412837906317312

Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene

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