Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens

Purpose – This paper aims to study the effect of different organic solvents on the extraction of pigments present in callus cultures of E. cinerascens. Design/methodology/approach – Attempts have been made to extract pigments from callus cultures through tissue culture system as an alternative repl...

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Main Authors:	Elias, Hashimah, Mat Taha, Rosna, Hasbullah, Nor Azlina, Othman, Rashidi, Mahmad, Noraini, Saleh, Azani, Abdullah, Sakinah
Format:	Article
Language:	English English English
Published:	Emerald Publishing Limited 2018
Subjects:	SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
Online Access:	http://irep.iium.edu.my/67642/ http://irep.iium.edu.my/67642/ http://irep.iium.edu.my/67642/ http://irep.iium.edu.my/67642/1/PRT%20RO1.pdf http://irep.iium.edu.my/67642/7/67642_Detection%20and%20quantification%20of%20natural%20pigments_scopus.pdf http://irep.iium.edu.my/67642/14/67642%20Detection%20and%20quantification%20of%20natural%20pigments%20extracted%20from%20callus%20of%20Echinocereus%20cinerascens_wos.pdf

id	iium-67642
recordtype	eprints
spelling	iium-676422019-03-13T06:57:03Z http://irep.iium.edu.my/67642/ Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens Elias, Hashimah Mat Taha, Rosna Hasbullah, Nor Azlina Othman, Rashidi Mahmad, Noraini Saleh, Azani Abdullah, Sakinah SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Purpose – This paper aims to study the effect of different organic solvents on the extraction of pigments present in callus cultures of E. cinerascens. Design/methodology/approach – Attempts have been made to extract pigments from callus cultures through tissue culture system as an alternative replacement for conventional plant cultivation as tissue culture provides unlimited supplies of plant samples. Callus of E. cinerascens was induced from stem explant cultured in Murashige and Skoog medium supplemented with a combination of 0.5 mg/L 6-benzylaminopurine and 0.5 mg/L a-naphthaleneacetic acid maintained under the photoperiod of 16 h light and 8 h dark. Fresh samples of the callus were harvested and dissolved in various types and concentrations of solvents such as 100 percent acetone, 80 percent acetone, 95 percent ethanol, 100 percent methanol and 90 percent methanol. Each of the mixtures was directly centrifuged to get clear supernatant containing pigments of interest. The pigments were detected and subsequently quantified via two simple techniques, ultraviolet-visible (UV-Vis) spectrophotometer and thin layer chromatography (TLC). Findings – UV-Vis spectrophotometer detected two families of pigments present in the callus cultures, namely, carotenoids (carotene and xanthophyll) and tetrapyrroles (chlorophyll a and b). Pigment contents in various solvent extractions were estimated using spectroscopic quantification equations established. Through TLC, spots were seen on the plates, and Rf values of each spot were assessed to indicate the possible existence of carotenoids and tetrapyrroles. Originality/value – This preliminary study offers significant finding for further advance research related on natural pigments extracted from E. cinerascens that would provide profits in future applications, especially in the food industry, medicine, agriculture, etc. Emerald Publishing Limited 2018-11-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67642/1/PRT%20RO1.pdf application/pdf en http://irep.iium.edu.my/67642/7/67642_Detection%20and%20quantification%20of%20natural%20pigments_scopus.pdf application/pdf en http://irep.iium.edu.my/67642/14/67642%20Detection%20and%20quantification%20of%20natural%20pigments%20extracted%20from%20callus%20of%20Echinocereus%20cinerascens_wos.pdf Elias, Hashimah and Mat Taha, Rosna and Hasbullah, Nor Azlina and Othman, Rashidi and Mahmad, Noraini and Saleh, Azani and Abdullah, Sakinah (2018) Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens. Pigment & Resin Technology, 47 (6). pp. 464-469. ISSN 0369-9420 https://www.emeraldinsight.com/doi/pdfplus/10.1108/PRT-11-2016-0103 10.1108/PRT-11-2016-0103
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
spellingShingle	SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Elias, Hashimah Mat Taha, Rosna Hasbullah, Nor Azlina Othman, Rashidi Mahmad, Noraini Saleh, Azani Abdullah, Sakinah Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens
description	Purpose – This paper aims to study the effect of different organic solvents on the extraction of pigments present in callus cultures of E. cinerascens. Design/methodology/approach – Attempts have been made to extract pigments from callus cultures through tissue culture system as an alternative replacement for conventional plant cultivation as tissue culture provides unlimited supplies of plant samples. Callus of E. cinerascens was induced from stem explant cultured in Murashige and Skoog medium supplemented with a combination of 0.5 mg/L 6-benzylaminopurine and 0.5 mg/L a-naphthaleneacetic acid maintained under the photoperiod of 16 h light and 8 h dark. Fresh samples of the callus were harvested and dissolved in various types and concentrations of solvents such as 100 percent acetone, 80 percent acetone, 95 percent ethanol, 100 percent methanol and 90 percent methanol. Each of the mixtures was directly centrifuged to get clear supernatant containing pigments of interest. The pigments were detected and subsequently quantified via two simple techniques, ultraviolet-visible (UV-Vis) spectrophotometer and thin layer chromatography (TLC). Findings – UV-Vis spectrophotometer detected two families of pigments present in the callus cultures, namely, carotenoids (carotene and xanthophyll) and tetrapyrroles (chlorophyll a and b). Pigment contents in various solvent extractions were estimated using spectroscopic quantification equations established. Through TLC, spots were seen on the plates, and Rf values of each spot were assessed to indicate the possible existence of carotenoids and tetrapyrroles. Originality/value – This preliminary study offers significant finding for further advance research related on natural pigments extracted from E. cinerascens that would provide profits in future applications, especially in the food industry, medicine, agriculture, etc.
format	Article
author	Elias, Hashimah Mat Taha, Rosna Hasbullah, Nor Azlina Othman, Rashidi Mahmad, Noraini Saleh, Azani Abdullah, Sakinah
author_facet	Elias, Hashimah Mat Taha, Rosna Hasbullah, Nor Azlina Othman, Rashidi Mahmad, Noraini Saleh, Azani Abdullah, Sakinah
author_sort	Elias, Hashimah
title	Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens
title_short	Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens
title_full	Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens
title_fullStr	Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens
title_full_unstemmed	Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens
title_sort	detection and quantification of natural pigments extracted from callus of echinocereus cinerascens
publisher	Emerald Publishing Limited
publishDate	2018
url	http://irep.iium.edu.my/67642/ http://irep.iium.edu.my/67642/ http://irep.iium.edu.my/67642/ http://irep.iium.edu.my/67642/1/PRT%20RO1.pdf http://irep.iium.edu.my/67642/7/67642_Detection%20and%20quantification%20of%20natural%20pigments_scopus.pdf http://irep.iium.edu.my/67642/14/67642%20Detection%20and%20quantification%20of%20natural%20pigments%20extracted%20from%20callus%20of%20Echinocereus%20cinerascens_wos.pdf
first_indexed	2023-09-18T21:36:01Z
last_indexed	2023-09-18T21:36:01Z
_version_	1777412825217499136

Detection and quantification of natural pigments extracted from callus of echinocereus cinerascens

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