The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus

Visual spectral sensitivity refers to the relative sensitivity of the eye towards different wavelengths of light. In fish, their eyes show specific adaptation in sensitivity to match the different light environments in their natural habitats. Sutchi catfish Pangasianodon hypophthalmus is one of the...

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Main Authors:	Nai, Han Tan, Mukai, Yukinori, Okawa, Ryo, Anraku, Kazuhiko
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture
Online Access:	http://irep.iium.edu.my/67517/ http://irep.iium.edu.my/67517/ http://irep.iium.edu.my/67517/1/67517_The%20spectrophotometric%20determination%20of%20visual.pdf

id	iium-67517
recordtype	eprints
spelling	iium-675172018-11-27T04:07:33Z http://irep.iium.edu.my/67517/ The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Visual spectral sensitivity refers to the relative sensitivity of the eye towards different wavelengths of light. In fish, their eyes show specific adaptation in sensitivity to match the different light environments in their natural habitats. Sutchi catfish Pangasianodon hypophthalmus is one of the commercially cultured freshwater fish species in Southeast Asia. The present study aimed to understand the visual spectral sensitivity of sutchi catfish through spectrophotometric analyses of the spectral absorbance of retinal wholemounts for the goal to establish appropriate lighting conditions in aquaculture system to improve growth performance of sutchi catfish. In the dark room, the retinae of sutchi catfish were isolated under a dissecting microscope with the help of a night vision goggles. The isolated retinae were cut into approximately 9 mm2 -size pieces. Each retina sample was then placed on a coverslip with a drop of saline solution and covered with another coverslip. Spectral absorbance of retinal wholemounts was measured by means of a portable modular spectrophotometer. A broad wavelength LED was used as the main light source, while blue (peak at 454 nm), green (520 nm), yellow (590 nm) and red (632 nm) LEDs were used as the supplementary light sources for flashing. Spectrophotometric measurement data were used to generate spectral absorbance curves. Comparison between the peaks of the curves showed that red light exposure induced a prominent shift of peak spectral absorbance towards shorter light wavelengths, suggesting the eyes of sutchi catfish had spectral sensitivity for light wavelength of red. 2018-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/67517/1/67517_The%20spectrophotometric%20determination%20of%20visual.pdf Nai, Han Tan and Mukai, Yukinori and Okawa, Ryo and Anraku, Kazuhiko (2018) The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus. In: International Conference on Agriculture, Animal Sciences and Food Technology (ICAFT) 2018, 30th-31st Oct. 2018, Kuala Terengganu, Terengganu. https://www.unisza.edu.my/icaft2018/index.php?lang=en
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture
spellingShingle	SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus
description	Visual spectral sensitivity refers to the relative sensitivity of the eye towards different wavelengths of light. In fish, their eyes show specific adaptation in sensitivity to match the different light environments in their natural habitats. Sutchi catfish Pangasianodon hypophthalmus is one of the commercially cultured freshwater fish species in Southeast Asia. The present study aimed to understand the visual spectral sensitivity of sutchi catfish through spectrophotometric analyses of the spectral absorbance of retinal wholemounts for the goal to establish appropriate lighting conditions in aquaculture system to improve growth performance of sutchi catfish. In the dark room, the retinae of sutchi catfish were isolated under a dissecting microscope with the help of a night vision goggles. The isolated retinae were cut into approximately 9 mm2 -size pieces. Each retina sample was then placed on a coverslip with a drop of saline solution and covered with another coverslip. Spectral absorbance of retinal wholemounts was measured by means of a portable modular spectrophotometer. A broad wavelength LED was used as the main light source, while blue (peak at 454 nm), green (520 nm), yellow (590 nm) and red (632 nm) LEDs were used as the supplementary light sources for flashing. Spectrophotometric measurement data were used to generate spectral absorbance curves. Comparison between the peaks of the curves showed that red light exposure induced a prominent shift of peak spectral absorbance towards shorter light wavelengths, suggesting the eyes of sutchi catfish had spectral sensitivity for light wavelength of red.
format	Conference or Workshop Item
author	Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko
author_facet	Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko
author_sort	Nai, Han Tan
title	The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus
title_short	The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus
title_full	The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus
title_fullStr	The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus
title_full_unstemmed	The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus
title_sort	spectrophotometric determination of visual spectral sensitivity of sutchi catfish pangasianodon hypophthalmus
publishDate	2018
url	http://irep.iium.edu.my/67517/ http://irep.iium.edu.my/67517/ http://irep.iium.edu.my/67517/1/67517_The%20spectrophotometric%20determination%20of%20visual.pdf
first_indexed	2023-09-18T21:35:50Z
last_indexed	2023-09-18T21:35:50Z
_version_	1777412814312308736

The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus

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