Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms

Mapping of the venoms proteins or now commonly called venomics have been the main approach towards understanding the venomous and destructive nature of venom proteins. These has led to high number of proteomics publications taking advantage of the LCMSMS technologies, that enable the accurate determ...

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Main Authors:	Othman, Iekhsan, Zainal Abidin, Syafiq Asnawi, Yusof, Fathin Athirah, Ismail, Ahmad Khaldun, Ahmad Rusmili, Muhamad Rusdi, Naidu, Rakesh
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/67156/ http://irep.iium.edu.my/67156/ http://irep.iium.edu.my/67156/1/67156_APPLICATIONS%20OF%20MASS%20SPECTROMETRY.pdf

id	iium-67156
recordtype	eprints
spelling	iium-671562018-10-31T01:51:19Z http://irep.iium.edu.my/67156/ Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms Othman, Iekhsan Zainal Abidin, Syafiq Asnawi Yusof, Fathin Athirah Ismail, Ahmad Khaldun Ahmad Rusmili, Muhamad Rusdi Naidu, Rakesh RM Therapeutics. Pharmacology RM300 Drugs and their action TP Chemical technology TP248.13 Biotechnology Mapping of the venoms proteins or now commonly called venomics have been the main approach towards understanding the venomous and destructive nature of venom proteins. These has led to high number of proteomics publications taking advantage of the LCMSMS technologies, that enable the accurate determinations of venoms constituents. The identification and purification of specific proteins such as L-amino acid oxidase and snake venom metalloproteinase (SVMP from crude venoms from Malaysian snakes such as the Malayan pit vipers (C. rhodostoma and C. purpureomaculatus) demonstrated selective cytotoxic activity on human colon cancer cells. Additionally, SVMP revealed strong inhibition of colon cancer cell migration as demonstrated from wound healing assay. HPLC subfractions with selective cytotoxic activity were analysed with LC-MS/MS and proteins that were detected with high confidence were purified such as L-amino acid oxidase (CR-LAAO) and snake venom C-type lectins (SNACLEC) rhodocetin and rhodocytin. LC-MS/MS and de novo sequencing of the isolated proteins from C. purpureomaculatus revealed amino acid variations highlighting a potential of a novel variant of each of the protein. CP-LAAO demonstrated strong cytotoxic activity and anti-proliferative activity, while CP-SVMP only affects the former. Caspase-3 induction activity was performed on CR-LAAO-, CP-LAAO- and CP-SVMP treated cells and revealed that stronger caspase-3 activity was detected from CR-LAAO and CP-LAAO at 24 hours of treatment and peaked at 48 hours. Bcl-2 cellular protein concentration assay was performed and significant drop of Bcl-2 concentration was identified from both CR- and CP-LAAO treated colon cancer cells. Morphological changes consistent with apoptosis on the LAAO treated cells further supported apoptosis induced cell death. Overall, these data provide evidence on the anticancer activity of several proteins from the venom of Malaysian pit vipers for therapeutic intervention of human colon cancer. We have also initiated anti-cancer studies on human brain cancer cells using venoms from Malaysian kraits, showed cytotoxic effects of B. candidus and B. fasciatus crude venoms and venom fractions in neuroblastoma cells. Preliminary data strongly suggested potent cytotoxic activities of the venom fractions on neuroblastoma cells. Overall, these data provided evidence on the application of LCMSMS in facilitating the potential anti-cancer activity from the venom of Malaysian snakes for therapeutic intervention. 2018-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/67156/1/67156_APPLICATIONS%20OF%20MASS%20SPECTROMETRY.pdf Othman, Iekhsan and Zainal Abidin, Syafiq Asnawi and Yusof, Fathin Athirah and Ismail, Ahmad Khaldun and Ahmad Rusmili, Muhamad Rusdi and Naidu, Rakesh (2018) Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms. In: ASEAN Marine Animals & Snake Envenoming (Poisoning) Management - AMSEM 2018, 23rd-26th October 2018, Yogjakarta, Indonesia. (Unpublished) http://mstamsem.blogspot.com/p/abstract.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action TP Chemical technology TP248.13 Biotechnology
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action TP Chemical technology TP248.13 Biotechnology Othman, Iekhsan Zainal Abidin, Syafiq Asnawi Yusof, Fathin Athirah Ismail, Ahmad Khaldun Ahmad Rusmili, Muhamad Rusdi Naidu, Rakesh Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms
description	Mapping of the venoms proteins or now commonly called venomics have been the main approach towards understanding the venomous and destructive nature of venom proteins. These has led to high number of proteomics publications taking advantage of the LCMSMS technologies, that enable the accurate determinations of venoms constituents. The identification and purification of specific proteins such as L-amino acid oxidase and snake venom metalloproteinase (SVMP from crude venoms from Malaysian snakes such as the Malayan pit vipers (C. rhodostoma and C. purpureomaculatus) demonstrated selective cytotoxic activity on human colon cancer cells. Additionally, SVMP revealed strong inhibition of colon cancer cell migration as demonstrated from wound healing assay. HPLC subfractions with selective cytotoxic activity were analysed with LC-MS/MS and proteins that were detected with high confidence were purified such as L-amino acid oxidase (CR-LAAO) and snake venom C-type lectins (SNACLEC) rhodocetin and rhodocytin. LC-MS/MS and de novo sequencing of the isolated proteins from C. purpureomaculatus revealed amino acid variations highlighting a potential of a novel variant of each of the protein. CP-LAAO demonstrated strong cytotoxic activity and anti-proliferative activity, while CP-SVMP only affects the former. Caspase-3 induction activity was performed on CR-LAAO-, CP-LAAO- and CP-SVMP treated cells and revealed that stronger caspase-3 activity was detected from CR-LAAO and CP-LAAO at 24 hours of treatment and peaked at 48 hours. Bcl-2 cellular protein concentration assay was performed and significant drop of Bcl-2 concentration was identified from both CR- and CP-LAAO treated colon cancer cells. Morphological changes consistent with apoptosis on the LAAO treated cells further supported apoptosis induced cell death. Overall, these data provide evidence on the anticancer activity of several proteins from the venom of Malaysian pit vipers for therapeutic intervention of human colon cancer. We have also initiated anti-cancer studies on human brain cancer cells using venoms from Malaysian kraits, showed cytotoxic effects of B. candidus and B. fasciatus crude venoms and venom fractions in neuroblastoma cells. Preliminary data strongly suggested potent cytotoxic activities of the venom fractions on neuroblastoma cells. Overall, these data provided evidence on the application of LCMSMS in facilitating the potential anti-cancer activity from the venom of Malaysian snakes for therapeutic intervention.
format	Conference or Workshop Item
author	Othman, Iekhsan Zainal Abidin, Syafiq Asnawi Yusof, Fathin Athirah Ismail, Ahmad Khaldun Ahmad Rusmili, Muhamad Rusdi Naidu, Rakesh
author_facet	Othman, Iekhsan Zainal Abidin, Syafiq Asnawi Yusof, Fathin Athirah Ismail, Ahmad Khaldun Ahmad Rusmili, Muhamad Rusdi Naidu, Rakesh
author_sort	Othman, Iekhsan
title	Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms
title_short	Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms
title_full	Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms
title_fullStr	Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms
title_full_unstemmed	Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms
title_sort	applications of mass spectrometry in identification of potential anticancer proteins from snake venoms
publishDate	2018
url	http://irep.iium.edu.my/67156/ http://irep.iium.edu.my/67156/ http://irep.iium.edu.my/67156/1/67156_APPLICATIONS%20OF%20MASS%20SPECTROMETRY.pdf
first_indexed	2023-09-18T21:35:21Z
last_indexed	2023-09-18T21:35:21Z
_version_	1777412783536603136

Applications of mass spectrometry in identification of potential anticancer proteins from snake venoms

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