Application of retina acid improves form and function of tissue engineered corneal construct

Retinoic acid has recently been shown to control the phenotype and extracellular matrix composition of corneal stromal cells cultured in vitro as monolayers. This study set out to investigate the effects of retinoic acid on human corneal keratocytes within a 3D environment. Human corneal keratocytes...

Full description

Bibliographic Details
Main Authors:	Zainal Abidin, Fadhilah, Gouveia, Ricardo M, Connon, Che J
Format:	Article
Language:	English English English
Published:	Taylor and Francis 2015
Subjects:	RE Ophthalmology
Online Access:	http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/1/Application%20of%20retinoic%20acid%20improves%20form%20and%20function%20of%20tissue%20engineered%20corneal%20construct.pdf http://irep.iium.edu.my/67141/7/Scopus%20-%20Application%20of%20retinoic%20acid.pdf http://irep.iium.edu.my/67141/8/WOS%20-%20Application%20of%20retinoic%20acid.pdf

id	iium-67141
recordtype	eprints
spelling	iium-671412018-12-14T03:14:32Z http://irep.iium.edu.my/67141/ Application of retina acid improves form and function of tissue engineered corneal construct Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J RE Ophthalmology Retinoic acid has recently been shown to control the phenotype and extracellular matrix composition of corneal stromal cells cultured in vitro as monolayers. This study set out to investigate the effects of retinoic acid on human corneal keratocytes within a 3D environment. Human corneal keratocytes were encapsulated in collagen gels, which were subsequently compressed under load, and cultured in serum-free media supplemented with 10 mM retinoic acid or DMSO vehicle for 30 days. Cell proliferation was quantified on selected days, while the expression of several important keratocytes markers was evaluated at day 30 using RT-PCR and immunoblotting. The weight and size of the collagen constructs were measured before and after hydration and contraction analyses. Retinoic acid enhanced keratocyte proliferation until day 30, whereas cells in control culture conditions showed reduced numbers after day 21. Both gene and protein expressions of keratocyte-characteristic proteoglycans (keratocan, lumican and decorin), corneal crystallins and collagen type I and V were significantly increased following retinoic acid supplementation. Retinoic acid also significantly reduced the expression of matrix metalloproteases 1, 3 and 9 while not increasing a-smooth muscle actin and fibronectin expression. Furthermore, these effects were also correlated with the ability of retinoic acid to significantly inhibit the contractility of keratocytes while allowing the build-up of corneal stromal extracellular matrix within the 3D constructs. Thus, retinoic acid supplementation represents a promising strategy to improve the phenotype of 3D-cultured keratocytes, and their usefulness as a model of corneal stroma for corneal biology and regenerative medicine applications. Taylor and Francis 2015-10-26 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67141/1/Application%20of%20retinoic%20acid%20improves%20form%20and%20function%20of%20tissue%20engineered%20corneal%20construct.pdf application/pdf en http://irep.iium.edu.my/67141/7/Scopus%20-%20Application%20of%20retinoic%20acid.pdf application/pdf en http://irep.iium.edu.my/67141/8/WOS%20-%20Application%20of%20retinoic%20acid.pdf Zainal Abidin, Fadhilah and Gouveia, Ricardo M and Connon, Che J (2015) Application of retina acid improves form and function of tissue engineered corneal construct. Organogenesis, 11 (3). pp. 122-136. ISSN 1547-6278 E-ISSN 1555-8592 https://www.tandfonline.com/doi/full/10.1080/15476278.2015.1093267 10.1080/15476278.2015.1093267
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RE Ophthalmology
spellingShingle	RE Ophthalmology Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J Application of retina acid improves form and function of tissue engineered corneal construct
description	Retinoic acid has recently been shown to control the phenotype and extracellular matrix composition of corneal stromal cells cultured in vitro as monolayers. This study set out to investigate the effects of retinoic acid on human corneal keratocytes within a 3D environment. Human corneal keratocytes were encapsulated in collagen gels, which were subsequently compressed under load, and cultured in serum-free media supplemented with 10 mM retinoic acid or DMSO vehicle for 30 days. Cell proliferation was quantified on selected days, while the expression of several important keratocytes markers was evaluated at day 30 using RT-PCR and immunoblotting. The weight and size of the collagen constructs were measured before and after hydration and contraction analyses. Retinoic acid enhanced keratocyte proliferation until day 30, whereas cells in control culture conditions showed reduced numbers after day 21. Both gene and protein expressions of keratocyte-characteristic proteoglycans (keratocan, lumican and decorin), corneal crystallins and collagen type I and V were significantly increased following retinoic acid supplementation. Retinoic acid also significantly reduced the expression of matrix metalloproteases 1, 3 and 9 while not increasing a-smooth muscle actin and fibronectin expression. Furthermore, these effects were also correlated with the ability of retinoic acid to significantly inhibit the contractility of keratocytes while allowing the build-up of corneal stromal extracellular matrix within the 3D constructs. Thus, retinoic acid supplementation represents a promising strategy to improve the phenotype of 3D-cultured keratocytes, and their usefulness as a model of corneal stroma for corneal biology and regenerative medicine applications.
format	Article
author	Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J
author_facet	Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J
author_sort	Zainal Abidin, Fadhilah
title	Application of retina acid improves form and function of tissue engineered corneal construct
title_short	Application of retina acid improves form and function of tissue engineered corneal construct
title_full	Application of retina acid improves form and function of tissue engineered corneal construct
title_fullStr	Application of retina acid improves form and function of tissue engineered corneal construct
title_full_unstemmed	Application of retina acid improves form and function of tissue engineered corneal construct
title_sort	application of retina acid improves form and function of tissue engineered corneal construct
publisher	Taylor and Francis
publishDate	2015
url	http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/1/Application%20of%20retinoic%20acid%20improves%20form%20and%20function%20of%20tissue%20engineered%20corneal%20construct.pdf http://irep.iium.edu.my/67141/7/Scopus%20-%20Application%20of%20retinoic%20acid.pdf http://irep.iium.edu.my/67141/8/WOS%20-%20Application%20of%20retinoic%20acid.pdf
first_indexed	2023-09-18T21:35:20Z
last_indexed	2023-09-18T21:35:20Z
_version_	1777412782236368896

Application of retina acid improves form and function of tissue engineered corneal construct

Similar Items