siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice

Tauopathy is a pathological hallmark of many neurodegenerative diseases. It is characterized by abnormal aggregates of pathological phosphotau and somatodendritic redistribution. One suggested strategy for treating tauopathy is to stimulate autophagy, hence, getting rid of these pathological protein...

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Main Authors:	Salama, Mohamed M., El-Desouky, Sara, Alsayed, Aziza, El-Hussiny, Mahmoud, Magdy, Khaled, Fekry, Emad, Shabka, Osama, El-Khodery, Sabry A., Youssef, Mohamed A., Sobh, Mohamed, Mohamed, Wael Mohamed Yousef
Format:	Article
Language:	English English English
Published:	Springer Nature Switzerland AG 2019
Subjects:	R Medicine (General) RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/67119/ http://irep.iium.edu.my/67119/ http://irep.iium.edu.my/67119/ http://irep.iium.edu.my/67119/1/mTOR.pdf http://irep.iium.edu.my/67119/7/67119_siRNA%20Blocking%20of%20Mammalian%20Target%20of%20Rapamycin%20%28mTOR%29%20Attenuates%20Pathology%20in%20Annonacin-Induced%20Tauopathy%20in%20Mice_wos.pdf http://irep.iium.edu.my/67119/13/67119_siRNA%20Blocking%20of%20Mammalian%20Target%20of%20Rapamycin%20mTOR_scopus.pdf

id	iium-67119
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spelling	iium-671192019-08-05T06:12:34Z http://irep.iium.edu.my/67119/ siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice Salama, Mohamed M. El-Desouky, Sara Alsayed, Aziza El-Hussiny, Mahmoud Magdy, Khaled Fekry, Emad Shabka, Osama El-Khodery, Sabry A. Youssef, Mohamed A. Sobh, Mohamed Mohamed, Wael Mohamed Yousef R Medicine (General) RM300 Drugs and their action Tauopathy is a pathological hallmark of many neurodegenerative diseases. It is characterized by abnormal aggregates of pathological phosphotau and somatodendritic redistribution. One suggested strategy for treating tauopathy is to stimulate autophagy, hence, getting rid of these pathological protein aggregates. One key controller of autophagy is mTOR. Since stimulation of mTOR leads to inhibition of autophagy, inhibitors of mTOR will cause stimulation of autophagy process. In this report, tauopathy was induced in mice using annonacin. Blocking of mTOR was achieved through stereotaxic injection of siRNA against mTOR. The behavioral and immunohistochemical evaluation revealed the development of tauopathy model as proven by deterioration of behavioral performance in open field test and significant tau aggregates in annonacin-treated mice. Blocking of mTOR revealed significant clearance of tau aggregates in the injected side; however, tau expression was not affected by mTOR blockage. Springer Nature Switzerland AG 2019-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67119/1/mTOR.pdf application/pdf en http://irep.iium.edu.my/67119/7/67119_siRNA%20Blocking%20of%20Mammalian%20Target%20of%20Rapamycin%20%28mTOR%29%20Attenuates%20Pathology%20in%20Annonacin-Induced%20Tauopathy%20in%20Mice_wos.pdf application/pdf en http://irep.iium.edu.my/67119/13/67119_siRNA%20Blocking%20of%20Mammalian%20Target%20of%20Rapamycin%20mTOR_scopus.pdf Salama, Mohamed M. and El-Desouky, Sara and Alsayed, Aziza and El-Hussiny, Mahmoud and Magdy, Khaled and Fekry, Emad and Shabka, Osama and El-Khodery, Sabry A. and Youssef, Mohamed A. and Sobh, Mohamed and Mohamed, Wael Mohamed Yousef (2019) siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice. Neurotoxicity Research, 35 (4). pp. 987-992. ISSN 1029-8428 E-ISSN 1476-3524 (In Press) https://link.springer.com/article/10.1007/s12640-018-9974-3 10.1007/s12640-018-9974-3
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	R Medicine (General) RM300 Drugs and their action
spellingShingle	R Medicine (General) RM300 Drugs and their action Salama, Mohamed M. El-Desouky, Sara Alsayed, Aziza El-Hussiny, Mahmoud Magdy, Khaled Fekry, Emad Shabka, Osama El-Khodery, Sabry A. Youssef, Mohamed A. Sobh, Mohamed Mohamed, Wael Mohamed Yousef siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice
description	Tauopathy is a pathological hallmark of many neurodegenerative diseases. It is characterized by abnormal aggregates of pathological phosphotau and somatodendritic redistribution. One suggested strategy for treating tauopathy is to stimulate autophagy, hence, getting rid of these pathological protein aggregates. One key controller of autophagy is mTOR. Since stimulation of mTOR leads to inhibition of autophagy, inhibitors of mTOR will cause stimulation of autophagy process. In this report, tauopathy was induced in mice using annonacin. Blocking of mTOR was achieved through stereotaxic injection of siRNA against mTOR. The behavioral and immunohistochemical evaluation revealed the development of tauopathy model as proven by deterioration of behavioral performance in open field test and significant tau aggregates in annonacin-treated mice. Blocking of mTOR revealed significant clearance of tau aggregates in the injected side; however, tau expression was not affected by mTOR blockage.
format	Article
author	Salama, Mohamed M. El-Desouky, Sara Alsayed, Aziza El-Hussiny, Mahmoud Magdy, Khaled Fekry, Emad Shabka, Osama El-Khodery, Sabry A. Youssef, Mohamed A. Sobh, Mohamed Mohamed, Wael Mohamed Yousef
author_facet	Salama, Mohamed M. El-Desouky, Sara Alsayed, Aziza El-Hussiny, Mahmoud Magdy, Khaled Fekry, Emad Shabka, Osama El-Khodery, Sabry A. Youssef, Mohamed A. Sobh, Mohamed Mohamed, Wael Mohamed Yousef
author_sort	Salama, Mohamed M.
title	siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice
title_short	siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice
title_full	siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice
title_fullStr	siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice
title_full_unstemmed	siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice
title_sort	sirna blocking of mammalian target of rapamycin (mtor) attenuates pathology in annonacin-induced tauopathy in mice
publisher	Springer Nature Switzerland AG
publishDate	2019
url	http://irep.iium.edu.my/67119/ http://irep.iium.edu.my/67119/ http://irep.iium.edu.my/67119/ http://irep.iium.edu.my/67119/1/mTOR.pdf http://irep.iium.edu.my/67119/7/67119_siRNA%20Blocking%20of%20Mammalian%20Target%20of%20Rapamycin%20%28mTOR%29%20Attenuates%20Pathology%20in%20Annonacin-Induced%20Tauopathy%20in%20Mice_wos.pdf http://irep.iium.edu.my/67119/13/67119_siRNA%20Blocking%20of%20Mammalian%20Target%20of%20Rapamycin%20mTOR_scopus.pdf
first_indexed	2023-09-18T21:35:18Z
last_indexed	2023-09-18T21:35:18Z
_version_	1777412780294406144

siRNA blocking of mammalian target of rapamycin (mTOR) attenuates pathology in annonacin-induced tauopathy in mice

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