In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum

Purpose: This study predicted three-dimensional (3D) protein structure of Cryptosporidium parvum subtilisin-like serine protease (CpSUB) using homology modelling as the approach of 3D structure prediction. Methods: CpSUB is an extracellular protein comprising of 1324 amino acid residues. Its amino...

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Main Authors:	Mat Yusof, Afzan, Md Isa, Muhammad Lokman
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/67099/ http://irep.iium.edu.my/67099/1/3rd%20AMDI%20International%20Biohealth%20Science%20Conference%20%28IBSC%29%202018.pdf

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spelling	iium-670992018-10-30T03:18:40Z http://irep.iium.edu.my/67099/ In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum Mat Yusof, Afzan Md Isa, Muhammad Lokman Q Science (General) Purpose: This study predicted three-dimensional (3D) protein structure of Cryptosporidium parvum subtilisin-like serine protease (CpSUB) using homology modelling as the approach of 3D structure prediction. Methods: CpSUB is an extracellular protein comprising of 1324 amino acid residues. Its amino acid sequence was computed to determine the physiochemical properties. 3D protein structure was developed by using MODELLER v9.18 software before subjected to model refinement and assessment. The refined model was then computed for functional annotation. Quality assessment analysis of the protein model for CpSUB through its PROCHECK Ramachandran Plot and other reliable protein structure assessment tools indicated that the selected protein model is a reliable model. Result: The result from functional annotation revealed that it is sorted to cd07473 superfamily or peptidase S8 family domain in subtilisin-like proteins. To our best knowledge, this protein model of CpSUB was first proposed for the modeling of 3D structure despite not having a crystallized protein structure deposited yet in Protein Data Bank (PDB). Conclusion: Thus, this study may provide understanding to the molecular and structural function of CpSUB. This predicted 3D model may be further studied for wet lab characterization studies and may be used for a prospective therapeutic anti-cryptosporidial agent. 2018-01-19 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/67099/1/3rd%20AMDI%20International%20Biohealth%20Science%20Conference%20%28IBSC%29%202018.pdf Mat Yusof, Afzan and Md Isa, Muhammad Lokman (2018) In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum. In: 3rd Advanced Medical & Dental Institute International Biohealth Sciences Conferences 2018, 19th-20th Jan. 2018, Kuching, Sarawak. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mat Yusof, Afzan Md Isa, Muhammad Lokman In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum
description	Purpose: This study predicted three-dimensional (3D) protein structure of Cryptosporidium parvum subtilisin-like serine protease (CpSUB) using homology modelling as the approach of 3D structure prediction. Methods: CpSUB is an extracellular protein comprising of 1324 amino acid residues. Its amino acid sequence was computed to determine the physiochemical properties. 3D protein structure was developed by using MODELLER v9.18 software before subjected to model refinement and assessment. The refined model was then computed for functional annotation. Quality assessment analysis of the protein model for CpSUB through its PROCHECK Ramachandran Plot and other reliable protein structure assessment tools indicated that the selected protein model is a reliable model. Result: The result from functional annotation revealed that it is sorted to cd07473 superfamily or peptidase S8 family domain in subtilisin-like proteins. To our best knowledge, this protein model of CpSUB was first proposed for the modeling of 3D structure despite not having a crystallized protein structure deposited yet in Protein Data Bank (PDB). Conclusion: Thus, this study may provide understanding to the molecular and structural function of CpSUB. This predicted 3D model may be further studied for wet lab characterization studies and may be used for a prospective therapeutic anti-cryptosporidial agent.
format	Conference or Workshop Item
author	Mat Yusof, Afzan Md Isa, Muhammad Lokman
author_facet	Mat Yusof, Afzan Md Isa, Muhammad Lokman
author_sort	Mat Yusof, Afzan
title	In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum
title_short	In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum
title_full	In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum
title_fullStr	In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum
title_full_unstemmed	In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum
title_sort	in silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from cryptosporidium parvum
publishDate	2018
url	http://irep.iium.edu.my/67099/ http://irep.iium.edu.my/67099/1/3rd%20AMDI%20International%20Biohealth%20Science%20Conference%20%28IBSC%29%202018.pdf
first_indexed	2023-09-18T21:35:16Z
last_indexed	2023-09-18T21:35:16Z
_version_	1777412778136436736

In silico sequence analysis, homology modeling and function annotation of putative subtilisin-like serine protease from Cryptosporidium parvum

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