Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection

The protozoan Cryptosporidium mainly infects the epithelial cells of colorectal region. The Infection of this species can be observed and confirmed by immunofluorescent staining method in vitro. Meanwhile, the onco-microRNA and tumour suppressor miRNA expression pattern of the epithelial cells upon...

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Main Authors:	Jainul, Mohammed Abdullah, Md Isa, Muhammad Lokman, Mat Yusof, Afzan
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/67087/ http://irep.iium.edu.my/67087/1/Abstract%20MSAB%20conference-%20Jainul%20et%20al.pdf

id	iium-67087
recordtype	eprints
spelling	iium-670872018-10-29T01:34:08Z http://irep.iium.edu.my/67087/ Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection Jainul, Mohammed Abdullah Md Isa, Muhammad Lokman Mat Yusof, Afzan Q Science (General) The protozoan Cryptosporidium mainly infects the epithelial cells of colorectal region. The Infection of this species can be observed and confirmed by immunofluorescent staining method in vitro. Meanwhile, the onco-microRNA and tumour suppressor miRNA expression pattern of the epithelial cells upon Cryptosporidium infection may reveal possible effect of Cryptosporidium in the onset and progression of cancer. In this study, two epithelial colorectal cancer cell lines HCT8 and HT29 were infected with Cryptosporidium parvum (C. parvum) and observed using direct immunofluorescent technique via fluorescein conjugated Vicia Villosa Lectin (VVL) staining. Then, the RT-qPCR was performed to observe the expression of miR-21 and miR-145 in infected cell lines. Data normalization of the miRNA expression was carried out using reference gene RNU44. The immunofluorescent micrographs exhibited the Cryptosporidium infection on both HCT-8 and HT-29 cell lines with green fluorescence. Upregulation of miR-21 and downregulation of normalized expression of miR-145 was observed in both of the cell lines upon Cryptosporidium infection. We found the expression of miR-21 to be increased and miR-145 greatly decreased in both cell lines upon infection. Our current observation suggests that, C. parvum can potentially infect the colorectal cancer cell lines and play critical role in regulating oncomiRNAs and tumor suppressor miRNAs. 2018 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/67087/1/Abstract%20MSAB%20conference-%20Jainul%20et%20al.pdf Jainul, Mohammed Abdullah and Md Isa, Muhammad Lokman and Mat Yusof, Afzan (2018) Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection. In: The 15th Symposium of Malaysian Society of Applied Biology 2018- Translating Applied Biology for Future Sustainability, 29th June- 1st July 2018, Melaka. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Jainul, Mohammed Abdullah Md Isa, Muhammad Lokman Mat Yusof, Afzan Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection
description	The protozoan Cryptosporidium mainly infects the epithelial cells of colorectal region. The Infection of this species can be observed and confirmed by immunofluorescent staining method in vitro. Meanwhile, the onco-microRNA and tumour suppressor miRNA expression pattern of the epithelial cells upon Cryptosporidium infection may reveal possible effect of Cryptosporidium in the onset and progression of cancer. In this study, two epithelial colorectal cancer cell lines HCT8 and HT29 were infected with Cryptosporidium parvum (C. parvum) and observed using direct immunofluorescent technique via fluorescein conjugated Vicia Villosa Lectin (VVL) staining. Then, the RT-qPCR was performed to observe the expression of miR-21 and miR-145 in infected cell lines. Data normalization of the miRNA expression was carried out using reference gene RNU44. The immunofluorescent micrographs exhibited the Cryptosporidium infection on both HCT-8 and HT-29 cell lines with green fluorescence. Upregulation of miR-21 and downregulation of normalized expression of miR-145 was observed in both of the cell lines upon Cryptosporidium infection. We found the expression of miR-21 to be increased and miR-145 greatly decreased in both cell lines upon infection. Our current observation suggests that, C. parvum can potentially infect the colorectal cancer cell lines and play critical role in regulating oncomiRNAs and tumor suppressor miRNAs.
format	Conference or Workshop Item
author	Jainul, Mohammed Abdullah Md Isa, Muhammad Lokman Mat Yusof, Afzan
author_facet	Jainul, Mohammed Abdullah Md Isa, Muhammad Lokman Mat Yusof, Afzan
author_sort	Jainul, Mohammed Abdullah
title	Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection
title_short	Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection
title_full	Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection
title_fullStr	Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection
title_full_unstemmed	Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection
title_sort	immunofluorescent staining and mirna expression of hct-8 and ht-29 cell lines upon cryptosporidium infection
publishDate	2018
url	http://irep.iium.edu.my/67087/ http://irep.iium.edu.my/67087/1/Abstract%20MSAB%20conference-%20Jainul%20et%20al.pdf
first_indexed	2023-09-18T21:35:15Z
last_indexed	2023-09-18T21:35:15Z
_version_	1777412776988246016

Immunofluorescent staining and miRNA expression of HCT-8 AND HT-29 cell lines upon Cryptosporidium infection

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