Shedding light on lipase stability in natural deep eutectic solvents
This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as gly...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Croation Society of Chemical Engineers
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/1/elgharbawy%20et%20al%202018.pdf http://irep.iium.edu.my/67015/7/67015_Shedding%20light%20on%20lipase%20stability%20in%20natural%20_scopus.pdf |
| Summary: | This study presents the potential role of natural deep eutectic solvents (NADESs) in
a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as
a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different
hydrogen bond donors such as glycerol and malonic acid and sugars (glucose,
fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases:
lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS,
from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin
from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead
150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The
results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent
as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 %
and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km)
of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend
achieved by NADES may be a promising approach for applications and further perspectives
as genuinely green industrial solvents. |
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