α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts

Entada spiralis Ridl. (Leguminosae), locally known as “Sintok” or “Beluru” is a tropical woody climber that grows widely in Malaysia. It is a valuable and well known plant in herbal medicine due to its various traditional and medicinal applications. Crude extracts were obtained from the stem bark...

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Main Authors:	Opeyemi Roheem, Fatimah, Mat So'ad, Siti Zaiton, Ahmed, Qamar Uddin, Mohd Hassan, Norazian
Format:	Article
Language:	English English
Published:	Universiti Putra Malaysia 2019
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/66971/ http://irep.iium.edu.my/66971/ http://irep.iium.edu.my/66971/1/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_article.pdf http://irep.iium.edu.my/66971/2/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_scopus.pdf

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recordtype	eprints
spelling	iium-669712019-06-13T03:15:04Z http://irep.iium.edu.my/66971/ α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Mohd Hassan, Norazian QD Chemistry Entada spiralis Ridl. (Leguminosae), locally known as “Sintok” or “Beluru” is a tropical woody climber that grows widely in Malaysia. It is a valuable and well known plant in herbal medicine due to its various traditional and medicinal applications. Crude extracts were obtained from the stem bark by using petroleum ether, chloroform and methanol as extracting solvents and were then biossayed for their biological potential. The antioxidant and alpha-glucosidase inhibitory activities of the extracts were assessed by using DPPH, ABTS, β-carotene and α-glucosidase inhibitory methods respectively. Qualitative analysis showed the presence of most of the phytochemicals in methanol extract; however, chloroform and petroleum ether extracts contained terpenoid and tannins as their major phytoconstituents respectively. Methanol extract contained the highest amount of total phenolics (42.5 ± 15.85 µg/ml) and flavonoids (28.94 ± 2.93 µg/ml), and showed the most potent α-glucosidase inhibitory activity with IC50 value of 20.67 µg/ml. The same methanol extract exhibited highest β-carotene bleaching inhibition (27% at 1 mg/ml), while methanol and chloroform extracts exhibited good radical scavenging activities dose dependently (IC50 37.29 ± 0.05, 90.84 ± 3.12 µg/ml respectively) against ABTS and DPPH radicals. Bioassay-guided silica gel column chromatography purification of the most active methanol extract afforded 3,4′,5,7- tetrahydroxy flavone (6 mg). The compound displayed promising inhibitory activity against free radicals as well as α-glucosidase enzyme. These results further suggest the traditional medicinal uses of E. spiralis Ridl. stem bark as a therapeutic agent against hyperglycemia. Universiti Putra Malaysia 2019-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66971/1/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_article.pdf application/pdf en http://irep.iium.edu.my/66971/2/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_scopus.pdf Opeyemi Roheem, Fatimah and Mat So'ad, Siti Zaiton and Ahmed, Qamar Uddin and Mohd Hassan, Norazian (2019) α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts. Pertanika - Journal of Tropical Agricultural Sciences, 42 (1). pp. 139-153. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2042%20(1)%20Feb.%202019/10%20JTAS-1267-2017.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Mohd Hassan, Norazian α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts
description	Entada spiralis Ridl. (Leguminosae), locally known as “Sintok” or “Beluru” is a tropical woody climber that grows widely in Malaysia. It is a valuable and well known plant in herbal medicine due to its various traditional and medicinal applications. Crude extracts were obtained from the stem bark by using petroleum ether, chloroform and methanol as extracting solvents and were then biossayed for their biological potential. The antioxidant and alpha-glucosidase inhibitory activities of the extracts were assessed by using DPPH, ABTS, β-carotene and α-glucosidase inhibitory methods respectively. Qualitative analysis showed the presence of most of the phytochemicals in methanol extract; however, chloroform and petroleum ether extracts contained terpenoid and tannins as their major phytoconstituents respectively. Methanol extract contained the highest amount of total phenolics (42.5 ± 15.85 µg/ml) and flavonoids (28.94 ± 2.93 µg/ml), and showed the most potent α-glucosidase inhibitory activity with IC50 value of 20.67 µg/ml. The same methanol extract exhibited highest β-carotene bleaching inhibition (27% at 1 mg/ml), while methanol and chloroform extracts exhibited good radical scavenging activities dose dependently (IC50 37.29 ± 0.05, 90.84 ± 3.12 µg/ml respectively) against ABTS and DPPH radicals. Bioassay-guided silica gel column chromatography purification of the most active methanol extract afforded 3,4′,5,7- tetrahydroxy flavone (6 mg). The compound displayed promising inhibitory activity against free radicals as well as α-glucosidase enzyme. These results further suggest the traditional medicinal uses of E. spiralis Ridl. stem bark as a therapeutic agent against hyperglycemia.
format	Article
author	Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Mohd Hassan, Norazian
author_facet	Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Mohd Hassan, Norazian
author_sort	Opeyemi Roheem, Fatimah
title	α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts
title_short	α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts
title_full	α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts
title_fullStr	α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts
title_full_unstemmed	α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts
title_sort	α - glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (sintok) stem bark extracts
publisher	Universiti Putra Malaysia
publishDate	2019
url	http://irep.iium.edu.my/66971/ http://irep.iium.edu.my/66971/ http://irep.iium.edu.my/66971/1/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_article.pdf http://irep.iium.edu.my/66971/2/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_scopus.pdf
first_indexed	2023-09-18T21:35:05Z
last_indexed	2023-09-18T21:35:05Z
_version_	1777412767003705344

α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts

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