α - Glucosidase inhibitory and antioxidant activities of entada spiralis ridl. (Sintok) stem bark extracts
Entada spiralis Ridl. (Leguminosae), locally known as “Sintok” or “Beluru” is a tropical woody climber that grows widely in Malaysia. It is a valuable and well known plant in herbal medicine due to its various traditional and medicinal applications. Crude extracts were obtained from the stem bark...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Universiti Putra Malaysia
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/66971/ http://irep.iium.edu.my/66971/ http://irep.iium.edu.my/66971/1/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_article.pdf http://irep.iium.edu.my/66971/2/66971_%CE%B1%20-%20Glucosidase%20inhibitory%20and%20antioxidant%20_scopus.pdf |
| Summary: | Entada spiralis Ridl. (Leguminosae), locally known as “Sintok” or “Beluru”
is a tropical woody climber that grows widely in Malaysia. It is a valuable
and well known plant in herbal medicine due to its various traditional and
medicinal applications. Crude extracts were obtained from the stem bark
by using petroleum ether, chloroform and methanol as extracting solvents
and were then biossayed for their biological potential. The antioxidant and
alpha-glucosidase inhibitory activities of the extracts were assessed by
using DPPH, ABTS, β-carotene and α-glucosidase inhibitory methods
respectively. Qualitative analysis showed the presence of most of the
phytochemicals in methanol extract; however, chloroform and petroleum
ether extracts contained terpenoid and tannins as their major
phytoconstituents respectively. Methanol extract contained the highest
amount of total phenolics (42.5 ± 15.85 µg/ml) and flavonoids (28.94 ±
2.93 µg/ml), and showed the most potent α-glucosidase inhibitory activity
with IC50 value of 20.67 µg/ml. The same methanol extract exhibited
highest β-carotene bleaching inhibition (27% at 1 mg/ml), while methanol
and chloroform extracts exhibited good radical scavenging activities dose
dependently (IC50 37.29 ± 0.05, 90.84 ± 3.12 µg/ml respectively)
against ABTS and DPPH radicals. Bioassay-guided silica gel column
chromatography purification of the most active methanol extract afforded
3,4′,5,7- tetrahydroxy flavone (6 mg). The compound displayed promising
inhibitory activity against free radicals as well as α-glucosidase enzyme.
These results further suggest the traditional medicinal uses of E. spiralis
Ridl. stem bark as a therapeutic agent against hyperglycemia. |
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