Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines
Naringin has been reported to possess diverse pharmacological properties, including anti-arthritic and anti-inflammatory activities. The aim of the present study was to determine the potential anti-inflammatory effect of naringin in a mouse model of carrageenan-induced pleurisy. A single dose of nar...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Nature Switzerland AG.
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/66957/ http://irep.iium.edu.my/66957/ http://irep.iium.edu.my/66957/ http://irep.iium.edu.my/66957/1/Naringin%20Attenuates%20the%20Development%20of%20Carrageenan-Induced%20Acute%20Lung%20Inflammation%20Through%20Inhibition%20of%20NF-%CE%BAb%2C%20STAT3%20and%20Pro-Inflammatory%20Mediators%20and%20Enhancement%20of%20I%CE%BAB%CE%B1%20and%20Anti-Inflammatory%20Cytokines.pdf http://irep.iium.edu.my/66957/7/66957_Scopus%20-%20Document%20details.pdf |
| Summary: | Naringin has been reported to possess diverse pharmacological properties, including anti-arthritic and anti-inflammatory activities. The aim of the present study was to determine the potential anti-inflammatory effect of naringin in a mouse model of carrageenan-induced pleurisy. A single dose of naringin (40 and 80 mg/kg) was administered per oral (p.o.) 1 h before carrageenan (Cg) administration. Pro- and anti-inflammatory cytokines were analysed in pleural fluid. We also assessed the effects of naringin on the expression levels of iNOS, inducible cyclooxygenase isoform (COX-2), ICAM-1, MIP-2, PGE2, STAT3, TGF-β1, nuclear factor kappa B (NF-κB) and inhibitor of kappa B (IκBα) in lung tissue. The histological examinations revealed anti-inflammatory effect of naringin while Cg group deteriorated. Naringin downregulated Th1 and upregulated Th2 cytokines. Western blot analyses revealed increased protein expression of NF-κB, STAT3 and COX-2 and decreased IκBα in response to Cg treatment, which were reversed by the treatment with naringin. In the Cg group, mRNA expression levels of pro-inflammatory mediators upregulated and anti-inflammatory mediators downregulated. Naringin reversed these actions. |
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