Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)
Biofilm complicates osteomyelitis as there is antibiotic resistance and toxicity involved. In order to overcome the challenges of current treatment, gentamicin, the current antibiotic of choice for osteomyelitis, is fused with Nigella sativa oil to form an emulsion. Its efficacy as anti-biofilm a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Microscopy Society of Malaysia (MSM)
2015
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/66948/ http://irep.iium.edu.my/66948/ http://irep.iium.edu.my/66948/1/MJMicroscopy-2015-12%20%282%29.pdf |
| Summary: | Biofilm complicates osteomyelitis as there is antibiotic resistance and toxicity involved. In
order to overcome the challenges of current treatment, gentamicin, the current antibiotic
of choice for osteomyelitis, is fused with Nigella sativa oil to form an emulsion. Its efficacy
as anti-biofilm agent is assessed using confocal laser scanning microscope (CLSM) against
different strains of biofilm-producing Staphylococcus aureus. These strains of S.aureus
were first allowed to express biofilm before being exposed for 24 hours to the emulsion with
(0.1% (w/v) gentamicin; 40.2% (v/v) N.sativa. Later, the emulsion was removed and the
biofilm was stained with fluorescence staining. The slides were viewed under CLSM at 100
times resolution. 3D images of biofilm were reconstructed, using Image J software, to
measure the thickness of biofilm and viability of bacteria cells. Results revealed that the
emulsion significantly reduced biofilm thickness compared to gentamicin and N.sativa
alone in all strains of S.aureus (Tukey’s test p < 0.05). The emulsion was also able to
produce more than 80% and 15% surface percentage (%) of non-viable (dead) bacteria in
the sensitive and resistant strain, respectively, at a significant level when compared to
gentamicin and N.sativa (Tukey’s test p < 0.05). As a conclusion, this new fusion of
gentamicin-N.sativa may be effective towards the biofilm of S.aureus, and can be developed
further as a new promising anti-biofilm agent in osteomyelitis. |
|---|