Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits

Lipids in foods are prone to oxidation that causes deterioration of food quality and formation of free radicals. Free radicals in food products can cause oxidative damage which could results in many chronic health diseases. Commercially available synthetic antioxidants used as foo...

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Main Authors:	Shuhaimen, Muhammad Shahrain, Abdullah, Erna Normaya, Omar, Muhammad Nor, Ahmad, Mohammad Norazmi
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/66834/ http://irep.iium.edu.my/66834/ http://irep.iium.edu.my/66834/1/66834_STATISTICAL%20ANALYSIS%20AND%20MOLECULAR.pdf

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recordtype	eprints
spelling	iium-668342019-01-31T01:06:42Z http://irep.iium.edu.my/66834/ Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits Shuhaimen, Muhammad Shahrain Abdullah, Erna Normaya Omar, Muhammad Nor Ahmad, Mohammad Norazmi QD Chemistry Lipids in foods are prone to oxidation that causes deterioration of food quality and formation of free radicals. Free radicals in food products can cause oxidative damage which could results in many chronic health diseases. Commercially available synthetic antioxidants used as food additives are reported to endanger health. Therefore, Halal natural antioxidants from plants are investigated. In this study, viscious liquid containing antioxidant properties was extracted from freeze dried cashew (Anacardium occidentale) apple using supercritical fluid extraction (SFE) with carbon dioxide (CO2) as the solvent. The extractions were optimized with response surface methodology (RSM) using the central composite rotatable design (CCRD). The effects of pressure (x1; 200-300 bars), time (x3; 30- 90 min), and temperature (x2; 30-50°C) were studied on the antioxidant activity of the liquid, measured using the DPPH inhibition assay. The statistical ana lysis was performed by ANOVA and the quadratic model obtained is significant (R2= 0.9858). Based on the RSM model, the optimal extraction conditions were obtained at 288.98 bar, 66.21 min, and 36.98°C that yields 70.3399% DPPH inhibition, which is in reas onable agreement with the validation test (n= 3) that yields the highest activity (71.5167±0.6684%). Based on the t-values, the ascending order of the effects of linearterms on cashew apple antioxidant activity was was temperature < time < pressure. The total phenolic content of the extract is 0.056 mg GAE/mL. The optimised extract was analysed using GC-MS and FT-IR for chemical compounds identification. Four prominent compounds were identified from the chromatogram. The antibacterial activity of the extract was tested against Gram-positive and Gram -negative bacteria. Molecular docking was used to study the interactions of the identified compounds from the extract with the bacteria. 2018-08-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/66834/1/66834_STATISTICAL%20ANALYSIS%20AND%20MOLECULAR.pdf Shuhaimen, Muhammad Shahrain and Abdullah, Erna Normaya and Omar, Muhammad Nor and Ahmad, Mohammad Norazmi (2018) Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits. In: International Conference of Analytical Sciences (SKAM31), 17th-19th August 2018, Kuantan, Pahang. http://www.iium.edu.my/skam31/wp-content/uploads/2018/08/SKAM31-PROGRAMME-BOOK-PART-1.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Shuhaimen, Muhammad Shahrain Abdullah, Erna Normaya Omar, Muhammad Nor Ahmad, Mohammad Norazmi Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits
description	Lipids in foods are prone to oxidation that causes deterioration of food quality and formation of free radicals. Free radicals in food products can cause oxidative damage which could results in many chronic health diseases. Commercially available synthetic antioxidants used as food additives are reported to endanger health. Therefore, Halal natural antioxidants from plants are investigated. In this study, viscious liquid containing antioxidant properties was extracted from freeze dried cashew (Anacardium occidentale) apple using supercritical fluid extraction (SFE) with carbon dioxide (CO2) as the solvent. The extractions were optimized with response surface methodology (RSM) using the central composite rotatable design (CCRD). The effects of pressure (x1; 200-300 bars), time (x3; 30- 90 min), and temperature (x2; 30-50°C) were studied on the antioxidant activity of the liquid, measured using the DPPH inhibition assay. The statistical ana lysis was performed by ANOVA and the quadratic model obtained is significant (R2= 0.9858). Based on the RSM model, the optimal extraction conditions were obtained at 288.98 bar, 66.21 min, and 36.98°C that yields 70.3399% DPPH inhibition, which is in reas onable agreement with the validation test (n= 3) that yields the highest activity (71.5167±0.6684%). Based on the t-values, the ascending order of the effects of linearterms on cashew apple antioxidant activity was was temperature < time < pressure. The total phenolic content of the extract is 0.056 mg GAE/mL. The optimised extract was analysed using GC-MS and FT-IR for chemical compounds identification. Four prominent compounds were identified from the chromatogram. The antibacterial activity of the extract was tested against Gram-positive and Gram -negative bacteria. Molecular docking was used to study the interactions of the identified compounds from the extract with the bacteria.
format	Conference or Workshop Item
author	Shuhaimen, Muhammad Shahrain Abdullah, Erna Normaya Omar, Muhammad Nor Ahmad, Mohammad Norazmi
author_facet	Shuhaimen, Muhammad Shahrain Abdullah, Erna Normaya Omar, Muhammad Nor Ahmad, Mohammad Norazmi
author_sort	Shuhaimen, Muhammad Shahrain
title	Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits
title_short	Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits
title_full	Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits
title_fullStr	Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits
title_full_unstemmed	Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits
title_sort	statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits
publishDate	2018
url	http://irep.iium.edu.my/66834/ http://irep.iium.edu.my/66834/ http://irep.iium.edu.my/66834/1/66834_STATISTICAL%20ANALYSIS%20AND%20MOLECULAR.pdf
first_indexed	2023-09-18T21:34:55Z
last_indexed	2023-09-18T21:34:55Z
_version_	1777412756239024128

Statistical analysis and molecular docking study on halal potential antioxidant from anacardium occidentale fruits

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