Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread

Introduction: Extracapsular spread (ECS) is one of the most important prognostic factors in oral squamous cell carcinoma (OSCC). Gamma Glutamyl Hydrolase (GGH) is a lysosomal enzyme which is involved in folate homeostasis. It is overexpressed in several human malignancies but its role in OSCC has ne...

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Main Authors:	Burhanudin, Nor Aszlitah, Kallarakkal, Thomas George
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/66747/ http://irep.iium.edu.my/66747/7/66747%20Expression%20Of%20Gamma%20Glutamyl%20Hydrolase.pdf

id	iium-66747
recordtype	eprints
spelling	iium-667472018-11-09T01:27:11Z http://irep.iium.edu.my/66747/ Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread Burhanudin, Nor Aszlitah Kallarakkal, Thomas George RB Pathology Introduction: Extracapsular spread (ECS) is one of the most important prognostic factors in oral squamous cell carcinoma (OSCC). Gamma Glutamyl Hydrolase (GGH) is a lysosomal enzyme which is involved in folate homeostasis. It is overexpressed in several human malignancies but its role in OSCC has never been reported. Carbonic Anhydrase 9 (CA9) is a transmembrane glycoprotein and is related to hypoxia. High expression of CA9 has been associated with poor prognosis in several tumours including OSCC. This study was aimed to investigate the expression of GGH and CA9 in OSCC and their potential use as biomarkers to predict ECS. Materials and Methods: Immunohistochemical staining with GGH and CA9 markers were performed in 35 cases of OSCC (19 with ECS and 16 without ECS) and 5 cases of normal mucosa. A semi-quantative index was used for immunoscoring. Association between the expression of these markers and ECS status were analyzed using chi-square test. Results: Immunohistochemical results indicated that GGH and CA9 were upregulated in OSCC. High GGH and CA9 expression were significantly associated with ECS (p<0.05). Combination of GGH and CA9 showed greater prognostic accuracy as compared to the individual markers. Conclusion(s): Both separately and in combination GGH and CA9 offer potential as prognostic biomarkers in OSCC and was thus reliable in segregating the OSCC cases based on ECS status. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/66747/7/66747%20Expression%20Of%20Gamma%20Glutamyl%20Hydrolase.pdf Burhanudin, Nor Aszlitah and Kallarakkal, Thomas George (2017) Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread. In: 1st IIUM International Dental Conference 2017 in Commemoration of the Kulliyyah of Dentistry 10th Anniversary (1st IIDC), 6th-8th October 2017, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Burhanudin, Nor Aszlitah Kallarakkal, Thomas George Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
description	Introduction: Extracapsular spread (ECS) is one of the most important prognostic factors in oral squamous cell carcinoma (OSCC). Gamma Glutamyl Hydrolase (GGH) is a lysosomal enzyme which is involved in folate homeostasis. It is overexpressed in several human malignancies but its role in OSCC has never been reported. Carbonic Anhydrase 9 (CA9) is a transmembrane glycoprotein and is related to hypoxia. High expression of CA9 has been associated with poor prognosis in several tumours including OSCC. This study was aimed to investigate the expression of GGH and CA9 in OSCC and their potential use as biomarkers to predict ECS. Materials and Methods: Immunohistochemical staining with GGH and CA9 markers were performed in 35 cases of OSCC (19 with ECS and 16 without ECS) and 5 cases of normal mucosa. A semi-quantative index was used for immunoscoring. Association between the expression of these markers and ECS status were analyzed using chi-square test. Results: Immunohistochemical results indicated that GGH and CA9 were upregulated in OSCC. High GGH and CA9 expression were significantly associated with ECS (p<0.05). Combination of GGH and CA9 showed greater prognostic accuracy as compared to the individual markers. Conclusion(s): Both separately and in combination GGH and CA9 offer potential as prognostic biomarkers in OSCC and was thus reliable in segregating the OSCC cases based on ECS status.
format	Conference or Workshop Item
author	Burhanudin, Nor Aszlitah Kallarakkal, Thomas George
author_facet	Burhanudin, Nor Aszlitah Kallarakkal, Thomas George
author_sort	Burhanudin, Nor Aszlitah
title	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_short	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_full	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_fullStr	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_full_unstemmed	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_sort	expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
publishDate	2017
url	http://irep.iium.edu.my/66747/ http://irep.iium.edu.my/66747/7/66747%20Expression%20Of%20Gamma%20Glutamyl%20Hydrolase.pdf
first_indexed	2023-09-18T21:34:48Z
last_indexed	2023-09-18T21:34:48Z
_version_	1777412748802523136

Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread

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