Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread

Introduction: Extracapsular spread (ECS) is one of the most important prognostic factors in oral squamous cell carcinoma (OSCC). Gamma Glutamyl Hydrolase (GGH) is a lysosomal enzyme which is involved in folate homeostasis. It is overexpressed in several human malignancies but its role in OSCC has ne...

Full description

Bibliographic Details
Main Authors:	Burhanudin, Nor Aszlitah, Kallarakkal, Thomas George
Format:	Article
Language:	English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017
Subjects:	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Online Access:	http://irep.iium.edu.my/66746/ http://irep.iium.edu.my/66746/ http://irep.iium.edu.my/66746/1/Expression%20of%20GGH%20and%20CA9%20in%20OSCC%20and%20their%20association%20with%20Extracapsular%20Spread.pdf

id	iium-66746
recordtype	eprints
spelling	iium-667462018-10-22T01:15:01Z http://irep.iium.edu.my/66746/ Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread Burhanudin, Nor Aszlitah Kallarakkal, Thomas George RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Introduction: Extracapsular spread (ECS) is one of the most important prognostic factors in oral squamous cell carcinoma (OSCC). Gamma Glutamyl Hydrolase (GGH) is a lysosomal enzyme which is involved in folate homeostasis. It is overexpressed in several human malignancies but its role in OSCC has never been reported. Carbonic Anhydrase 9 (CA9) is a transmembrane glycoprotein and is related to hypoxia. High expression of CA9 has been associated with poor prognosis in several tumours including OSCC. This study was aimed to investigate the expression of GGH and CA9 in OSCC and their potential use as biomarkers to predict ECS. Materials and Methods: Immunohistochemical staining with GGH and CA9 markers were performed in 35 cases of OSCC (19 with ECS and 16 without ECS) and 5 cases of normal mucosa. A semi-quantative index was used for immunoscoring. Association between the expression of these markers and ECS status were analyzed using chi-square test. Results: Immunohistochemical results indicated that GGH and CA9 were upregulated in OSCC. High GGH and CA9 expression were significantly associated with ECS (p<0.05). Combination of GGH and CA9 showed greater prognostic accuracy as compared to the individual markers. Conclusion(s): Both separately and in combination GGH and CA9 offer potential as prognostic biomarkers in OSCC and was thus reliable in segregating the OSCC cases based on ECS status. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66746/1/Expression%20of%20GGH%20and%20CA9%20in%20OSCC%20and%20their%20association%20with%20Extracapsular%20Spread.pdf Burhanudin, Nor Aszlitah and Kallarakkal, Thomas George (2017) Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread. International Medical Journal of Malaysia, 16 (Supplementary Issus no 2). p. 1. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2016%20No%201%20Supp%202/ID19_IMJM-Vol16-Supp2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
spellingShingle	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Burhanudin, Nor Aszlitah Kallarakkal, Thomas George Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
description	Introduction: Extracapsular spread (ECS) is one of the most important prognostic factors in oral squamous cell carcinoma (OSCC). Gamma Glutamyl Hydrolase (GGH) is a lysosomal enzyme which is involved in folate homeostasis. It is overexpressed in several human malignancies but its role in OSCC has never been reported. Carbonic Anhydrase 9 (CA9) is a transmembrane glycoprotein and is related to hypoxia. High expression of CA9 has been associated with poor prognosis in several tumours including OSCC. This study was aimed to investigate the expression of GGH and CA9 in OSCC and their potential use as biomarkers to predict ECS. Materials and Methods: Immunohistochemical staining with GGH and CA9 markers were performed in 35 cases of OSCC (19 with ECS and 16 without ECS) and 5 cases of normal mucosa. A semi-quantative index was used for immunoscoring. Association between the expression of these markers and ECS status were analyzed using chi-square test. Results: Immunohistochemical results indicated that GGH and CA9 were upregulated in OSCC. High GGH and CA9 expression were significantly associated with ECS (p<0.05). Combination of GGH and CA9 showed greater prognostic accuracy as compared to the individual markers. Conclusion(s): Both separately and in combination GGH and CA9 offer potential as prognostic biomarkers in OSCC and was thus reliable in segregating the OSCC cases based on ECS status.
format	Article
author	Burhanudin, Nor Aszlitah Kallarakkal, Thomas George
author_facet	Burhanudin, Nor Aszlitah Kallarakkal, Thomas George
author_sort	Burhanudin, Nor Aszlitah
title	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_short	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_full	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_fullStr	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_full_unstemmed	Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
title_sort	expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/66746/ http://irep.iium.edu.my/66746/ http://irep.iium.edu.my/66746/1/Expression%20of%20GGH%20and%20CA9%20in%20OSCC%20and%20their%20association%20with%20Extracapsular%20Spread.pdf
first_indexed	2023-09-18T21:34:48Z
last_indexed	2023-09-18T21:34:48Z
_version_	1777412748644188160

Expression of gamma glutamyl hydrolase and carbonic anhydrase 9 in oral squamous cell carcinoma and their association with extracapsular spread

Similar Items