Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach

The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions namely hexane, chloroform and n-butanol fractions were evaluated for DPPH radical scavenging ef...

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Main Authors:	Ahmed, Qamar Uddin, Alhassan, Alhassan Muhammad, Khatib, Alfi, Shah, Syed Adnan Ali, Hasan, Muhammad Mahmudul, Sarian, Murni Nazira
Format:	Article
Language:	English English English
Published:	MDPI 2018
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/66719/ http://irep.iium.edu.my/66719/ http://irep.iium.edu.my/66719/ http://irep.iium.edu.my/66719/1/66719_Antiradical%20and%20Xanthine%20Oxidase.pdf http://irep.iium.edu.my/66719/2/66719_Antiradical%20and%20xanthine%20oxidase%20inhibitory_SCOPUS.pdf http://irep.iium.edu.my/66719/3/66719_Antiradical%20and%20xanthine%20oxidase%20inhibitory_WOS.pdf

id	iium-66719
recordtype	eprints
spelling	iium-667192019-01-23T03:56:36Z http://irep.iium.edu.my/66719/ Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Khatib, Alfi Shah, Syed Adnan Ali Hasan, Muhammad Mahmudul Sarian, Murni Nazira Q Science (General) The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions namely hexane, chloroform and n-butanol fractions were evaluated for DPPH radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC50 (4.14 ± 0.21 μg/mL) (p<0.05) as well as xanthine oxidase inhibitory activity with IC50 (64.84 ± 3.93 μg/mL) (p<0.05). Afzelechin 3-O-alpha-L-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction. MDPI 2018-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66719/1/66719_Antiradical%20and%20Xanthine%20Oxidase.pdf application/pdf en http://irep.iium.edu.my/66719/2/66719_Antiradical%20and%20xanthine%20oxidase%20inhibitory_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/66719/3/66719_Antiradical%20and%20xanthine%20oxidase%20inhibitory_WOS.pdf Ahmed, Qamar Uddin and Alhassan, Alhassan Muhammad and Khatib, Alfi and Shah, Syed Adnan Ali and Hasan, Muhammad Mahmudul and Sarian, Murni Nazira (2018) Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach. Antioxidants, 7 (10). pp. 1-16. E-ISSN 2076-3921 https://www.mdpi.com/2076-3921/7/10/137 10.3390/antiox7100137
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Khatib, Alfi Shah, Syed Adnan Ali Hasan, Muhammad Mahmudul Sarian, Murni Nazira Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach
description	The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions namely hexane, chloroform and n-butanol fractions were evaluated for DPPH radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC50 (4.14 ± 0.21 μg/mL) (p<0.05) as well as xanthine oxidase inhibitory activity with IC50 (64.84 ± 3.93 μg/mL) (p<0.05). Afzelechin 3-O-alpha-L-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction.
format	Article
author	Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Khatib, Alfi Shah, Syed Adnan Ali Hasan, Muhammad Mahmudul Sarian, Murni Nazira
author_facet	Ahmed, Qamar Uddin Alhassan, Alhassan Muhammad Khatib, Alfi Shah, Syed Adnan Ali Hasan, Muhammad Mahmudul Sarian, Murni Nazira
author_sort	Ahmed, Qamar Uddin
title	Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach
title_short	Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach
title_full	Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach
title_fullStr	Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach
title_full_unstemmed	Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach
title_sort	antiradical and xanthine oxidase inhibitory activity evaluations of averrhoa bilimbi l. leaves and identification of bioactive constituents through lc-qtof-ms/ms and molecular docking approach
publisher	MDPI
publishDate	2018
url	http://irep.iium.edu.my/66719/ http://irep.iium.edu.my/66719/ http://irep.iium.edu.my/66719/ http://irep.iium.edu.my/66719/1/66719_Antiradical%20and%20Xanthine%20Oxidase.pdf http://irep.iium.edu.my/66719/2/66719_Antiradical%20and%20xanthine%20oxidase%20inhibitory_SCOPUS.pdf http://irep.iium.edu.my/66719/3/66719_Antiradical%20and%20xanthine%20oxidase%20inhibitory_WOS.pdf
first_indexed	2023-09-18T21:34:45Z
last_indexed	2023-09-18T21:34:45Z
_version_	1777412746175840256

Antiradical and xanthine oxidase inhibitory activity evaluations of Averrhoa bilimbi L. leaves and identification of bioactive constituents through LC-QTOF-MS/MS and molecular docking approach

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