Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract

Introduction:Breast cancer becomes the global health problem throughout the world. It is rated as the second most common cancer occurrence after lung cancer and the leading cause of death among women. The rising of breast cancer cases years by years is really worrying. Due to the side effects of cur...

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Main Authors:	Khatib, Alfi, Hashim, Ridzwan, Mohammad, Mardhiah, Draman, Samsul, Abdul Jamil, Muhammad Mahadi, Johan, Muhammad Farid, Hassan, Habibah
Format:	Monograph
Language:	English
Published:	2018
Subjects:	SF Animal culture
Online Access:	http://irep.iium.edu.my/66648/ http://irep.iium.edu.my/66648/1/UNDERSTANDING%20OF%20ANTI-CANCER%20PROPERTIES%20OF%20NEOLAMARCKIA%20CADAMBA%20LEAVES%20ON%20BREAST%20CANCER%20PROSPECTIVE%20THERAPEUTIC%20NEOLAMARCKIA%20CADAMBA%20EXTRACT%20%281%29.pdf

id	iium-66648
recordtype	eprints
spelling	iium-666482019-01-07T02:24:51Z http://irep.iium.edu.my/66648/ Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract Khatib, Alfi Hashim, Ridzwan Mohammad, Mardhiah Draman, Samsul Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Hassan, Habibah SF Animal culture Introduction:Breast cancer becomes the global health problem throughout the world. It is rated as the second most common cancer occurrence after lung cancer and the leading cause of death among women. The rising of breast cancer cases years by years is really worrying. Due to the side effects of current treatments and resistance towards chemotropic drugs, it is important to evaluate new chemopreventive agents from various sources particularly plants. Method: Neolamarckia cadamba leaves were rinsed, dried and pulverised into coarse powder. The powdered leaves were sonicated in ethanol. The filtered extract were concentrated and freeze dried. In in vitro analysis, Neolamarckia cadamba leaves extracts (NCE) were tested on breast cancer cell line (MCF-7). Different concentration of sample extracts were administered on MCF-7 cells to find the 50% inhibitory concentration [IC50] after 72 hours of treatment. The treated MCF-7 cells were observed under microscope to observe the morphological changes. Cytotoxicity assay was conducted on normal cells to test the extract toxicity using IC50 value. Flowcytometric analysis were conducted to evaluate apoptosis induction and cell cycle arrest using the IC50 value of extracts against MCF-7 cancer cells.Quantitative PCR (qPCR) were conducted to investigate the gene expression with regards to induction of apoptosis and cell cycle arrest in treated MCF-7 cells. Phytochemical screening were conducted by TPC and TFC assay as well as GCMS analysis.Result: MCF-7 treated cells showed decreased in cell growth with increased concentration of NCE. The value of IC50 obtain was at 0.2 mg/ml. Under microscopic observation, the MCF-7 treated cells showed the features of apoptosis including cell shrinkage, cell blebbing and apoptotic cells No significant toxic effect were observed on both normal fibroblast cell line after treated with NCE. Moreover, flowcytometric analysis on apoptosis induction demonstrated that NCE induced apoptosis and cell cycle arrest at G0/G1 phase. qPCR assay of gene expression showed up-regulated of apoptosis related genes (cytochrome C, Baxcaspase 9 and caspase 7) and down-regulated of Bcl-2and caspase 8were suggested that NCE induced apoptosis via intrinsic mitochondrial pathway. Meanwhile, the up-regulated of p21 and down-regulated of CDK2 indicated that the NCE cause cell cycle arrest. Phenol and flavonoid compound were detected in the NCE. Several compoundswere presented in the NCE includephenol,2,4-bis(1,1-dimethyllethyl)-, levoglucosan, d-Glucose, beta-D-(+)-Talopyranose, hexadecanoic acid, myo-Inositol, oleic acid and octadecanoic acid.Conclusion: Neolamarckia cadamba leaves have a potential to act as chemopreventive agent for breast cancer treatment. 2018 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/66648/1/UNDERSTANDING%20OF%20ANTI-CANCER%20PROPERTIES%20OF%20NEOLAMARCKIA%20CADAMBA%20LEAVES%20ON%20BREAST%20CANCER%20PROSPECTIVE%20THERAPEUTIC%20NEOLAMARCKIA%20CADAMBA%20EXTRACT%20%281%29.pdf Khatib, Alfi and Hashim, Ridzwan and Mohammad, Mardhiah and Draman, Samsul and Abdul Jamil, Muhammad Mahadi and Johan, Muhammad Farid and Hassan, Habibah (2018) Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract. Research Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SF Animal culture
spellingShingle	SF Animal culture Khatib, Alfi Hashim, Ridzwan Mohammad, Mardhiah Draman, Samsul Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Hassan, Habibah Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract
description	Introduction:Breast cancer becomes the global health problem throughout the world. It is rated as the second most common cancer occurrence after lung cancer and the leading cause of death among women. The rising of breast cancer cases years by years is really worrying. Due to the side effects of current treatments and resistance towards chemotropic drugs, it is important to evaluate new chemopreventive agents from various sources particularly plants. Method: Neolamarckia cadamba leaves were rinsed, dried and pulverised into coarse powder. The powdered leaves were sonicated in ethanol. The filtered extract were concentrated and freeze dried. In in vitro analysis, Neolamarckia cadamba leaves extracts (NCE) were tested on breast cancer cell line (MCF-7). Different concentration of sample extracts were administered on MCF-7 cells to find the 50% inhibitory concentration [IC50] after 72 hours of treatment. The treated MCF-7 cells were observed under microscope to observe the morphological changes. Cytotoxicity assay was conducted on normal cells to test the extract toxicity using IC50 value. Flowcytometric analysis were conducted to evaluate apoptosis induction and cell cycle arrest using the IC50 value of extracts against MCF-7 cancer cells.Quantitative PCR (qPCR) were conducted to investigate the gene expression with regards to induction of apoptosis and cell cycle arrest in treated MCF-7 cells. Phytochemical screening were conducted by TPC and TFC assay as well as GCMS analysis.Result: MCF-7 treated cells showed decreased in cell growth with increased concentration of NCE. The value of IC50 obtain was at 0.2 mg/ml. Under microscopic observation, the MCF-7 treated cells showed the features of apoptosis including cell shrinkage, cell blebbing and apoptotic cells No significant toxic effect were observed on both normal fibroblast cell line after treated with NCE. Moreover, flowcytometric analysis on apoptosis induction demonstrated that NCE induced apoptosis and cell cycle arrest at G0/G1 phase. qPCR assay of gene expression showed up-regulated of apoptosis related genes (cytochrome C, Baxcaspase 9 and caspase 7) and down-regulated of Bcl-2and caspase 8were suggested that NCE induced apoptosis via intrinsic mitochondrial pathway. Meanwhile, the up-regulated of p21 and down-regulated of CDK2 indicated that the NCE cause cell cycle arrest. Phenol and flavonoid compound were detected in the NCE. Several compoundswere presented in the NCE includephenol,2,4-bis(1,1-dimethyllethyl)-, levoglucosan, d-Glucose, beta-D-(+)-Talopyranose, hexadecanoic acid, myo-Inositol, oleic acid and octadecanoic acid.Conclusion: Neolamarckia cadamba leaves have a potential to act as chemopreventive agent for breast cancer treatment.
format	Monograph
author	Khatib, Alfi Hashim, Ridzwan Mohammad, Mardhiah Draman, Samsul Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Hassan, Habibah
author_facet	Khatib, Alfi Hashim, Ridzwan Mohammad, Mardhiah Draman, Samsul Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Hassan, Habibah
author_sort	Khatib, Alfi
title	Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract
title_short	Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract
title_full	Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract
title_fullStr	Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract
title_full_unstemmed	Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract
title_sort	understanding of anti-cancer properties of neolamarckia cadamba leaves on breast cancer: prospective therapeutic neolamarckia cadamba extract
publishDate	2018
url	http://irep.iium.edu.my/66648/ http://irep.iium.edu.my/66648/1/UNDERSTANDING%20OF%20ANTI-CANCER%20PROPERTIES%20OF%20NEOLAMARCKIA%20CADAMBA%20LEAVES%20ON%20BREAST%20CANCER%20PROSPECTIVE%20THERAPEUTIC%20NEOLAMARCKIA%20CADAMBA%20EXTRACT%20%281%29.pdf
first_indexed	2023-09-18T21:34:39Z
last_indexed	2023-09-18T21:34:39Z
_version_	1777412739717660672

Understanding of anti-cancer properties of Neolamarckia cadamba leaves on breast cancer: prospective therapeutic Neolamarckia cadamba extract

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