Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)

Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)

Previous study reported that plant natural product promotes stem cell growth. Flaxseed (Linum usitatissimum) contains numerous compounds recognized for its health benefits. This study aimed to investigate the biological effects of flaxseed crude extract on stem cells obtained from human exfoliate...

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Main Authors: Nordin, Nur Sazwi, Mokhtar@Makhtar, Khairani Idah, Md Isa, Muhannd Lokman, Syed Osman Idid, Syed Zahir Idid, Lestari, Widya, Mustafa Al-Ahmad, Basma Ezzat, Arief Ichwan, Solachuddin Jauhari, Darnis, Deny Susanti, Ahmad, Azlina, Kannan, Thirumulu Ponnuraj
Format: Article
Language:English
English
Published: Ectodermal Dysplasia Group-Turkey 2018
Subjects:
RK Dentistry
Online Access:http://irep.iium.edu.my/66347/
http://irep.iium.edu.my/66347/
http://irep.iium.edu.my/66347/1/47D18_587-Nur%20Sazwi%20et%20al%202018.pdf
http://irep.iium.edu.my/66347/7/66347_Flaxseed%20ethanolic%20crude%20extract%20influences%20growth%20of%20stem%20cells%20from%20human%20exfoliated%20deciduous%20teeth%20%28SHED%29.pdf
id iium-66347
recordtype eprints
spelling iium-663472019-11-12T02:37:18Z http://irep.iium.edu.my/66347/ Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED) Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Md Isa, Muhannd Lokman Syed Osman Idid, Syed Zahir Idid Lestari, Widya Mustafa Al-Ahmad, Basma Ezzat Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Ahmad, Azlina Kannan, Thirumulu Ponnuraj RK Dentistry Previous study reported that plant natural product promotes stem cell growth. Flaxseed (Linum usitatissimum) contains numerous compounds recognized for its health benefits. This study aimed to investigate the biological effects of flaxseed crude extract on stem cells obtained from human exfoliated deciduous teeth (SHED). Whole flaxseeds were ground and extracted with absolute ethanol using soxhlet extractor. The effects of flaxseed crude extract on SHED were assessed at concentration 1, 2, 4, 8 and 16 mg/ml for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity describe as population doubling time (PDT) using alamarBlue assay. The fatty acid composition of flaxseed was analysed using gas chromatographymass spectrometry (GCMS) instrumental technique.Although insignificant, flaxseed at concentration up to 4 mg/ml slightly increased SHED proliferation activity while maintaining cell viability and morphology. However, 8 mg/ml of flaxseed inhibited cell viability and proliferation activity, and changed the cell morphology. GCMS analysis revealed the presence of linolenic acid, linoleic acid, palmitic acid and stearic acid. Overall, ethanolic crude extract of flaxseed at concentration up to 4 mg/ml slightly enhanced the growth and maintained the morphology of SHED. Ectodermal Dysplasia Group-Turkey 2018-04-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66347/1/47D18_587-Nur%20Sazwi%20et%20al%202018.pdf application/pdf en http://irep.iium.edu.my/66347/7/66347_Flaxseed%20ethanolic%20crude%20extract%20influences%20growth%20of%20stem%20cells%20from%20human%20exfoliated%20deciduous%20teeth%20%28SHED%29.pdf Nordin, Nur Sazwi and Mokhtar@Makhtar, Khairani Idah and Md Isa, Muhannd Lokman and Syed Osman Idid, Syed Zahir Idid and Lestari, Widya and Mustafa Al-Ahmad, Basma Ezzat and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Ahmad, Azlina and Kannan, Thirumulu Ponnuraj (2018) Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED). Journal of International Dental and Medical Research, 11 (2). pp. 643-649. ISSN 1309-100X E-ISSN 1309-100X http://www.jidmr.com
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic RK Dentistry
spellingShingle RK Dentistry
Nordin, Nur Sazwi
Mokhtar@Makhtar, Khairani Idah
Md Isa, Muhannd Lokman
Syed Osman Idid, Syed Zahir Idid
Lestari, Widya
Mustafa Al-Ahmad, Basma Ezzat
Arief Ichwan, Solachuddin Jauhari
Darnis, Deny Susanti
Ahmad, Azlina
Kannan, Thirumulu Ponnuraj
Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)
description Previous study reported that plant natural product promotes stem cell growth. Flaxseed (Linum usitatissimum) contains numerous compounds recognized for its health benefits. This study aimed to investigate the biological effects of flaxseed crude extract on stem cells obtained from human exfoliated deciduous teeth (SHED). Whole flaxseeds were ground and extracted with absolute ethanol using soxhlet extractor. The effects of flaxseed crude extract on SHED were assessed at concentration 1, 2, 4, 8 and 16 mg/ml for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity describe as population doubling time (PDT) using alamarBlue assay. The fatty acid composition of flaxseed was analysed using gas chromatographymass spectrometry (GCMS) instrumental technique.Although insignificant, flaxseed at concentration up to 4 mg/ml slightly increased SHED proliferation activity while maintaining cell viability and morphology. However, 8 mg/ml of flaxseed inhibited cell viability and proliferation activity, and changed the cell morphology. GCMS analysis revealed the presence of linolenic acid, linoleic acid, palmitic acid and stearic acid. Overall, ethanolic crude extract of flaxseed at concentration up to 4 mg/ml slightly enhanced the growth and maintained the morphology of SHED.
format Article
author Nordin, Nur Sazwi
Mokhtar@Makhtar, Khairani Idah
Md Isa, Muhannd Lokman
Syed Osman Idid, Syed Zahir Idid
Lestari, Widya
Mustafa Al-Ahmad, Basma Ezzat
Arief Ichwan, Solachuddin Jauhari
Darnis, Deny Susanti
Ahmad, Azlina
Kannan, Thirumulu Ponnuraj
author_facet Nordin, Nur Sazwi
Mokhtar@Makhtar, Khairani Idah
Md Isa, Muhannd Lokman
Syed Osman Idid, Syed Zahir Idid
Lestari, Widya
Mustafa Al-Ahmad, Basma Ezzat
Arief Ichwan, Solachuddin Jauhari
Darnis, Deny Susanti
Ahmad, Azlina
Kannan, Thirumulu Ponnuraj
author_sort Nordin, Nur Sazwi
title Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)
title_short Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)
title_full Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)
title_fullStr Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)
title_full_unstemmed Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)
title_sort flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (shed)
publisher Ectodermal Dysplasia Group-Turkey
publishDate 2018
url http://irep.iium.edu.my/66347/
http://irep.iium.edu.my/66347/
http://irep.iium.edu.my/66347/1/47D18_587-Nur%20Sazwi%20et%20al%202018.pdf
http://irep.iium.edu.my/66347/7/66347_Flaxseed%20ethanolic%20crude%20extract%20influences%20growth%20of%20stem%20cells%20from%20human%20exfoliated%20deciduous%20teeth%20%28SHED%29.pdf
first_indexed 2023-09-18T21:34:11Z
last_indexed 2023-09-18T21:34:11Z
_version_ 1777412710535790592

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