Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept

Expression of beta-human chorionic gonadotropin (bHCG) mRNA in early cleavage cell stages is important in predicting embryo viability at blastocyst stage of development. Embryo viability is crucial for in vitro fertilization (IVF) treatment to be successful in terms of increment of implantation an...

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Main Authors:	Md Isa, Muhammad Lokman, Mat Yusof, Afzan, Ramli, Roszaman, Ahmad Arifin, Syamsul, Abdul Wahab, Azantee Yazmie
Format:	Article
Language:	English
Published:	International Islamic University Malaysia 2018
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/66346/ http://irep.iium.edu.my/66346/ http://irep.iium.edu.my/66346/1/bhcg.pdf

id	iium-66346
recordtype	eprints
spelling	iium-663462019-03-03T20:54:20Z http://irep.iium.edu.my/66346/ Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept Md Isa, Muhammad Lokman Mat Yusof, Afzan Ramli, Roszaman Ahmad Arifin, Syamsul Abdul Wahab, Azantee Yazmie Q Science (General) Expression of beta-human chorionic gonadotropin (bHCG) mRNA in early cleavage cell stages is important in predicting embryo viability at blastocyst stage of development. Embryo viability is crucial for in vitro fertilization (IVF) treatment to be successful in terms of increment of implantation and pregnancy rate. In order to establish fertilization outside the human body, optimum condition mimicking the natural body environment like hormones and growth factors as well as impeccable timing must be established. Imam Muslim narrated from Hudhayfa ibn Asad that the Prophet Muhammad (SAW) said: “After the sperm-andovum drop (nut.fa) has been [in the uterus] forty-two days, Allah sends it an angel that gives it form and fashions its hearing, sight, skin, flesh, and skeleton”. Therefore the aim of this study is to find the significant association of bHCG expression with early cell stage cleavages and its relationship as predictive marker for potential embryo implantation. Our study focuses on leftover frozen embryos from eight patients consists of six pregnant patients and two non-pregnant patients. We assessed the human bHCG mRNA expression at different cell cleavage stages in these frozen embryos using reverse transcriptasepolymerase chain reaction (RT-PCR). Our results have shown three out of eight patients with five to ten cells of blastomeres were expressed with bHCG. This study indicated that bHCG was expressed on frozethawed late cleavage stage of embryos in IVF patients. International Islamic University Malaysia 2018-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66346/1/bhcg.pdf Md Isa, Muhammad Lokman and Mat Yusof, Afzan and Ramli, Roszaman and Ahmad Arifin, Syamsul and Abdul Wahab, Azantee Yazmie (2018) Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept. International Medical Journal Malaysia, 17 (Special Issue 2). pp. 165-170. ISSN 1823-4631 http://iiumedic.net/imjm/v1/about-imjm/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Md Isa, Muhammad Lokman Mat Yusof, Afzan Ramli, Roszaman Ahmad Arifin, Syamsul Abdul Wahab, Azantee Yazmie Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
description	Expression of beta-human chorionic gonadotropin (bHCG) mRNA in early cleavage cell stages is important in predicting embryo viability at blastocyst stage of development. Embryo viability is crucial for in vitro fertilization (IVF) treatment to be successful in terms of increment of implantation and pregnancy rate. In order to establish fertilization outside the human body, optimum condition mimicking the natural body environment like hormones and growth factors as well as impeccable timing must be established. Imam Muslim narrated from Hudhayfa ibn Asad that the Prophet Muhammad (SAW) said: “After the sperm-andovum drop (nut.fa) has been [in the uterus] forty-two days, Allah sends it an angel that gives it form and fashions its hearing, sight, skin, flesh, and skeleton”. Therefore the aim of this study is to find the significant association of bHCG expression with early cell stage cleavages and its relationship as predictive marker for potential embryo implantation. Our study focuses on leftover frozen embryos from eight patients consists of six pregnant patients and two non-pregnant patients. We assessed the human bHCG mRNA expression at different cell cleavage stages in these frozen embryos using reverse transcriptasepolymerase chain reaction (RT-PCR). Our results have shown three out of eight patients with five to ten cells of blastomeres were expressed with bHCG. This study indicated that bHCG was expressed on frozethawed late cleavage stage of embryos in IVF patients.
format	Article
author	Md Isa, Muhammad Lokman Mat Yusof, Afzan Ramli, Roszaman Ahmad Arifin, Syamsul Abdul Wahab, Azantee Yazmie
author_facet	Md Isa, Muhammad Lokman Mat Yusof, Afzan Ramli, Roszaman Ahmad Arifin, Syamsul Abdul Wahab, Azantee Yazmie
author_sort	Md Isa, Muhammad Lokman
title	Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
title_short	Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
title_full	Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
title_fullStr	Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
title_full_unstemmed	Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
title_sort	identification of beta human chorionic gonadotrophin (bhcg) expression in frozen cleavage embryo of in vitro fertilization (ivf) patients: an essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
publisher	International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/66346/ http://irep.iium.edu.my/66346/ http://irep.iium.edu.my/66346/1/bhcg.pdf
first_indexed	2023-09-18T21:34:11Z
last_indexed	2023-09-18T21:34:11Z
_version_	1777412710388989952

Identification of beta human chorionic gonadotrophin (bHCG) expression in frozen cleavage embryo of in vitro fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept

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