Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs

Background: There is no histological study evaluating the effects of insulin-containing cream on skin injury. The goal of this study was to examine the effects of insulin-containing creams on wound healing. Methods: Creams consisting of nine parts of oil and one part of aqueous phase (9:1) mixed wit...

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Main Authors:	Mohamed, Nur-Aliana Hidayah, Mokhtar, Rafidah Hanim, Al-Ani, Imad Matloub Dally, Ayob, Azizi, Misran, Misni
Format:	Article
Language:	English English
Published:	Universitas Indonesia 2018
Subjects:	QM Human anatomy QP Physiology RB Pathology RL Dermatology
Online Access:	http://irep.iium.edu.my/66214/ http://irep.iium.edu.my/66214/ http://irep.iium.edu.my/66214/ http://irep.iium.edu.my/66214/1/66214_Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream_article.pdf http://irep.iium.edu.my/66214/7/66214%20Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream%20as%20Evidenced%20from%20the%20Morphological%20Evaluation%20in%20Guinea%20Pigs_wos.pdf

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spelling	iium-662142019-01-31T02:08:52Z http://irep.iium.edu.my/66214/ Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs Mohamed, Nur-Aliana Hidayah Mokhtar, Rafidah Hanim Al-Ani, Imad Matloub Dally Ayob, Azizi Misran, Misni QM Human anatomy QP Physiology RB Pathology RL Dermatology Background: There is no histological study evaluating the effects of insulin-containing cream on skin injury. The goal of this study was to examine the effects of insulin-containing creams on wound healing. Methods: Creams consisting of nine parts of oil and one part of aqueous phase (9:1) mixed with 1.5 mL human insulin were prepared. Eighteen male guinea pigs were divided into three groups; the control (9:1 C) group received cream without insulin. The experimental groups received Humulin N (9:1 N) and Humulin R (9:1 R) respectively. A 1 cm2 wound of 1-2 cm thickness was created in the skin. Each animal received 0.5 g of the respective creams which was topically applied once a day for 14 days. The progress of wound healing was monitored daily. Skin tissues were excised at the 14th days from the wound sites and processed for light microscopy. Results: Skin wound treated with the long acting insulin Humulin N had an accelerated wound healing process with restoration of vascular network, increased collagen deposition and early complete wound remodeling. Conclusion: Insulin cream with long acting mechanism facilitates in normalizing cell permeability, promoting vascularization, reducing exudation and stimulate proliferation of cells. These properties render insulin cream suitable for expediting wound healing. Universitas Indonesia 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66214/1/66214_Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream_article.pdf application/pdf en http://irep.iium.edu.my/66214/7/66214%20Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream%20as%20Evidenced%20from%20the%20Morphological%20Evaluation%20in%20Guinea%20Pigs_wos.pdf Mohamed, Nur-Aliana Hidayah and Mokhtar, Rafidah Hanim and Al-Ani, Imad Matloub Dally and Ayob, Azizi and Misran, Misni (2018) Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs. Makara Journal of Health Research, 22 (2). pp. 88-94. ISSN 2356-3664 E-ISSN 2356-3656 http://journal.ui.ac.id/index.php/health/article/view/9714/pdf 10.7454/msk.v22i2.9714
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QM Human anatomy QP Physiology RB Pathology RL Dermatology
spellingShingle	QM Human anatomy QP Physiology RB Pathology RL Dermatology Mohamed, Nur-Aliana Hidayah Mokhtar, Rafidah Hanim Al-Ani, Imad Matloub Dally Ayob, Azizi Misran, Misni Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs
description	Background: There is no histological study evaluating the effects of insulin-containing cream on skin injury. The goal of this study was to examine the effects of insulin-containing creams on wound healing. Methods: Creams consisting of nine parts of oil and one part of aqueous phase (9:1) mixed with 1.5 mL human insulin were prepared. Eighteen male guinea pigs were divided into three groups; the control (9:1 C) group received cream without insulin. The experimental groups received Humulin N (9:1 N) and Humulin R (9:1 R) respectively. A 1 cm2 wound of 1-2 cm thickness was created in the skin. Each animal received 0.5 g of the respective creams which was topically applied once a day for 14 days. The progress of wound healing was monitored daily. Skin tissues were excised at the 14th days from the wound sites and processed for light microscopy. Results: Skin wound treated with the long acting insulin Humulin N had an accelerated wound healing process with restoration of vascular network, increased collagen deposition and early complete wound remodeling. Conclusion: Insulin cream with long acting mechanism facilitates in normalizing cell permeability, promoting vascularization, reducing exudation and stimulate proliferation of cells. These properties render insulin cream suitable for expediting wound healing.
format	Article
author	Mohamed, Nur-Aliana Hidayah Mokhtar, Rafidah Hanim Al-Ani, Imad Matloub Dally Ayob, Azizi Misran, Misni
author_facet	Mohamed, Nur-Aliana Hidayah Mokhtar, Rafidah Hanim Al-Ani, Imad Matloub Dally Ayob, Azizi Misran, Misni
author_sort	Mohamed, Nur-Aliana Hidayah
title	Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs
title_short	Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs
title_full	Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs
title_fullStr	Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs
title_full_unstemmed	Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs
title_sort	improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs
publisher	Universitas Indonesia
publishDate	2018
url	http://irep.iium.edu.my/66214/ http://irep.iium.edu.my/66214/ http://irep.iium.edu.my/66214/ http://irep.iium.edu.my/66214/1/66214_Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream_article.pdf http://irep.iium.edu.my/66214/7/66214%20Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream%20as%20Evidenced%20from%20the%20Morphological%20Evaluation%20in%20Guinea%20Pigs_wos.pdf
first_indexed	2023-09-18T21:33:58Z
last_indexed	2023-09-18T21:33:58Z
_version_	1777412696612798464

Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs

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