Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity
BACKGROUND: Antioxidant natural products and chemoprevention are considered nowadays as an effective approach against health various disorders and diseases induced by oxidative stress or free radicals. OBJECTIVE: The aim of this study was to assess the hepato- and nephroprotective activity of a...
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| Language: | English |
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Wolters Kluwer - Medknow Publications and Media Pvt. Ltd
2015
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| Online Access: | http://irep.iium.edu.my/66082/ http://irep.iium.edu.my/66082/ http://irep.iium.edu.my/66082/ http://irep.iium.edu.my/66082/1/Hepatorenal%20protective%20effect%20of%20Antistax%20%C2%AE%20against%20chemically-induced%20toxicity.pdf |
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iium-660822018-10-02T04:02:39Z http://irep.iium.edu.my/66082/ Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity Ahmed, Atallah F. Al‑Yousef, Hanan M. Al‑Qahtani, Jawaher H. Al‑Said, Mansour S. Ashour, Abdelkader Elbadawy Abbas Al‑Sohaibani, Mohammed , Syed Rafatullah RM300 Drugs and their action BACKGROUND: Antioxidant natural products and chemoprevention are considered nowadays as an effective approach against health various disorders and diseases induced by oxidative stress or free radicals. OBJECTIVE: The aim of this study was to assess the hepato- and nephroprotective activity of a standardized red vine leaf aqueous extract AS195 (Antistax(®)). METHODS: The protective activity of AS195 (100 mg/kg) was investigated on carbon tetrachloride (CCl4)-intoxicated rats in comparison with silymarin. The flavonoid/proanthocyanidin nature of AS195 was identified by phytochemical and nuclear magnetic resonance (NMR) analyses, while its total phenol/proanthocyanidin/flavonoid content and antioxidant activity were determined by Folin-Ciocalteau, vanillin-sulfuric acid, AlCl3, and 2, 2-diphenyl-2-picrylhydrazyl radical scavenging assays, respectively. RESULTS: Relative to the control CCl4 -intoxicated group, pretreatment with AS195 could significantly suppressed the elevated serum levels of alanine aminotransferase, alkaline phosphatase, γ-glutamyl transferase, total cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, triglycerides, bilirubin, creatinine, uric acid, and calcium, whereas it significantly increased the diminished serum levels of high-density lipoprotein cholesterol, albumin and total protein. Moreover, AS195 significantly decreased malondialdehyde formation in the tissues of liver and kidney, whereas it significantly elevated and nonprotein sulfhydryl groups, compared with the intoxicated control. The improvement in biochemical parameters by AS195 was obviously observed and further confirmed by restoration of normal histological features in the two organs. CONCLUSIONS: The results of the present study revealed the capacity of AS195 to enhance the recovery from xenobiotic-induced hepatorenal toxicity initiated by free radicals. Wolters Kluwer - Medknow Publications and Media Pvt. Ltd 2015-05-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66082/1/Hepatorenal%20protective%20effect%20of%20Antistax%20%C2%AE%20against%20chemically-induced%20toxicity.pdf Ahmed, Atallah F. and Al‑Yousef, Hanan M. and Al‑Qahtani, Jawaher H. and Al‑Said, Mansour S. and Ashour, Abdelkader Elbadawy Abbas and Al‑Sohaibani, Mohammed and , Syed Rafatullah (2015) Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity. Pharmacognosy Magazine, 11 (42 (Supplement 1)). S173-S181. ISSN 0973-1296 E-ISSN 0976-4062 http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=42;spage=173;epage=181;aulast=Ahmed;type=0 10.4103/0973-1296.157726 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
RM300 Drugs and their action |
| spellingShingle |
RM300 Drugs and their action Ahmed, Atallah F. Al‑Yousef, Hanan M. Al‑Qahtani, Jawaher H. Al‑Said, Mansour S. Ashour, Abdelkader Elbadawy Abbas Al‑Sohaibani, Mohammed , Syed Rafatullah Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity |
| description |
BACKGROUND:
Antioxidant natural products and chemoprevention are considered nowadays as an effective approach against health various disorders and diseases induced by oxidative stress or free radicals.
OBJECTIVE:
The aim of this study was to assess the hepato- and nephroprotective activity of a standardized red vine leaf aqueous extract AS195 (Antistax(®)).
METHODS:
The protective activity of AS195 (100 mg/kg) was investigated on carbon tetrachloride (CCl4)-intoxicated rats in comparison with silymarin. The flavonoid/proanthocyanidin nature of AS195 was identified by phytochemical and nuclear magnetic resonance (NMR) analyses, while its total phenol/proanthocyanidin/flavonoid content and antioxidant activity were determined by Folin-Ciocalteau, vanillin-sulfuric acid, AlCl3, and 2, 2-diphenyl-2-picrylhydrazyl radical scavenging assays, respectively.
RESULTS:
Relative to the control CCl4 -intoxicated group, pretreatment with AS195 could significantly suppressed the elevated serum levels of alanine aminotransferase, alkaline phosphatase, γ-glutamyl transferase, total cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, triglycerides, bilirubin, creatinine, uric acid, and calcium, whereas it significantly increased the diminished serum levels of high-density lipoprotein cholesterol, albumin and total protein. Moreover, AS195 significantly decreased malondialdehyde formation in the tissues of liver and kidney, whereas it significantly elevated and nonprotein sulfhydryl groups, compared with the intoxicated control. The improvement in biochemical parameters by AS195 was obviously observed and further confirmed by restoration of normal histological features in the two organs.
CONCLUSIONS:
The results of the present study revealed the capacity of AS195 to enhance the recovery from xenobiotic-induced hepatorenal toxicity initiated by free radicals. |
| format |
Article |
| author |
Ahmed, Atallah F. Al‑Yousef, Hanan M. Al‑Qahtani, Jawaher H. Al‑Said, Mansour S. Ashour, Abdelkader Elbadawy Abbas Al‑Sohaibani, Mohammed , Syed Rafatullah |
| author_facet |
Ahmed, Atallah F. Al‑Yousef, Hanan M. Al‑Qahtani, Jawaher H. Al‑Said, Mansour S. Ashour, Abdelkader Elbadawy Abbas Al‑Sohaibani, Mohammed , Syed Rafatullah |
| author_sort |
Ahmed, Atallah F. |
| title |
Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity |
| title_short |
Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity |
| title_full |
Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity |
| title_fullStr |
Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity |
| title_full_unstemmed |
Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity |
| title_sort |
hepatorenal protective effect of antistax(®) against chemically-induced toxicity |
| publisher |
Wolters Kluwer - Medknow Publications and Media Pvt. Ltd |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/66082/ http://irep.iium.edu.my/66082/ http://irep.iium.edu.my/66082/ http://irep.iium.edu.my/66082/1/Hepatorenal%20protective%20effect%20of%20Antistax%20%C2%AE%20against%20chemically-induced%20toxicity.pdf |
| first_indexed |
2023-09-18T21:33:45Z |
| last_indexed |
2023-09-18T21:33:45Z |
| _version_ |
1777412682841849856 |