Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice

INTRODUCTION: This study explored the adaptive changes in pancreatic islets by assessing the maternal pancreatic β-cell mass (PβCM), islets size, number, shape, distribution and vascularity using the anti-GAD65 Ab as a marker in pregnant mice. MATERIALS AND METHODS: Fourty female Swiss-Webster mic...

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Main Authors:	Al-asadi, Alaa K., Al-Habib, May F., Al-Kawaz, Ula M., Selman, Mohammad Oda, Al-Ani, Imad Matloub Dally
Format:	Article
Language:	English English
Published:	International Islamic University Malaysia 2018
Subjects:	QM Human anatomy QP Physiology RB Pathology
Online Access:	http://irep.iium.edu.my/65606/ http://irep.iium.edu.my/65606/ http://irep.iium.edu.my/65606/1/65606_Morphometric%20study%20of%20changes%20in%20GAD%2065.pdf http://irep.iium.edu.my/65606/2/65606_Morphometric%20study%20of%20changes%20in%20GAD%2065_WOS.pdf

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spelling	iium-656062019-01-30T08:26:06Z http://irep.iium.edu.my/65606/ Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice Al-asadi, Alaa K. Al-Habib, May F. Al-Kawaz, Ula M. Selman, Mohammad Oda Al-Ani, Imad Matloub Dally QM Human anatomy QP Physiology RB Pathology INTRODUCTION: This study explored the adaptive changes in pancreatic islets by assessing the maternal pancreatic β-cell mass (PβCM), islets size, number, shape, distribution and vascularity using the anti-GAD65 Ab as a marker in pregnant mice. MATERIALS AND METHODS: Fourty female Swiss-Webster mice were randomly divided into two age matched groups of 20 animals each, pregnant and control (non-pregnant) groups. The pregnant mice were sacrificed at Day 19 of gestation and the control group was sacrificed 19 days after observation in the same environmental conditions. Specimens of pancreata were fixed in 10% formal-saline, and processed for light microscopy. Paraffin sections were stained with chromogen DAB. Image scope (APERIO) with Image J software was used for morphometric study. RESULTS: Islets cells showed specific reaction with GAD65 Ab. There was an increase in the cellularity and vascularity of the PβCM. A significant increase in the islets area of the pregnant group ranging between 32-723 μm2 in comparison with the control group that ranged between 5–210 μm2, the islet size and numbers also increased; there were numerous newly formed islets and coalescence of adjacent islets, positive reactivity of β-cells toward anti- GAD65 during pregnancy, the mean of positive granulation in PβCM in pregnant group was 8273.5±SE 831.35 while in control group was 34±SE 0.2. CONCLUSION: The changes for alteration in PβCM such as increase in cells number, size, positive anti-GAD65 reactivity and their vascularity during pregnancy is related to the adaptation for hormonal and nutritional demands of both foetus and mother. International Islamic University Malaysia 2018-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65606/1/65606_Morphometric%20study%20of%20changes%20in%20GAD%2065.pdf application/pdf en http://irep.iium.edu.my/65606/2/65606_Morphometric%20study%20of%20changes%20in%20GAD%2065_WOS.pdf Al-asadi, Alaa K. and Al-Habib, May F. and Al-Kawaz, Ula M. and Selman, Mohammad Oda and Al-Ani, Imad Matloub Dally (2018) Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice. International Medical Journal Malaysia (IMJM), 17 (2). pp. 83-90. ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/volume_17_no_2/11-Morphometric-study.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QM Human anatomy QP Physiology RB Pathology
spellingShingle	QM Human anatomy QP Physiology RB Pathology Al-asadi, Alaa K. Al-Habib, May F. Al-Kawaz, Ula M. Selman, Mohammad Oda Al-Ani, Imad Matloub Dally Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice
description	INTRODUCTION: This study explored the adaptive changes in pancreatic islets by assessing the maternal pancreatic β-cell mass (PβCM), islets size, number, shape, distribution and vascularity using the anti-GAD65 Ab as a marker in pregnant mice. MATERIALS AND METHODS: Fourty female Swiss-Webster mice were randomly divided into two age matched groups of 20 animals each, pregnant and control (non-pregnant) groups. The pregnant mice were sacrificed at Day 19 of gestation and the control group was sacrificed 19 days after observation in the same environmental conditions. Specimens of pancreata were fixed in 10% formal-saline, and processed for light microscopy. Paraffin sections were stained with chromogen DAB. Image scope (APERIO) with Image J software was used for morphometric study. RESULTS: Islets cells showed specific reaction with GAD65 Ab. There was an increase in the cellularity and vascularity of the PβCM. A significant increase in the islets area of the pregnant group ranging between 32-723 μm2 in comparison with the control group that ranged between 5–210 μm2, the islet size and numbers also increased; there were numerous newly formed islets and coalescence of adjacent islets, positive reactivity of β-cells toward anti- GAD65 during pregnancy, the mean of positive granulation in PβCM in pregnant group was 8273.5±SE 831.35 while in control group was 34±SE 0.2. CONCLUSION: The changes for alteration in PβCM such as increase in cells number, size, positive anti-GAD65 reactivity and their vascularity during pregnancy is related to the adaptation for hormonal and nutritional demands of both foetus and mother.
format	Article
author	Al-asadi, Alaa K. Al-Habib, May F. Al-Kawaz, Ula M. Selman, Mohammad Oda Al-Ani, Imad Matloub Dally
author_facet	Al-asadi, Alaa K. Al-Habib, May F. Al-Kawaz, Ula M. Selman, Mohammad Oda Al-Ani, Imad Matloub Dally
author_sort	Al-asadi, Alaa K.
title	Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice
title_short	Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice
title_full	Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice
title_fullStr	Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice
title_full_unstemmed	Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice
title_sort	morphometric study of changes in gad 65 expressing beta cell mass in the islets of langerhans in pregnant mice
publisher	International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/65606/ http://irep.iium.edu.my/65606/ http://irep.iium.edu.my/65606/1/65606_Morphometric%20study%20of%20changes%20in%20GAD%2065.pdf http://irep.iium.edu.my/65606/2/65606_Morphometric%20study%20of%20changes%20in%20GAD%2065_WOS.pdf
first_indexed	2023-09-18T21:33:05Z
last_indexed	2023-09-18T21:33:05Z
_version_	1777412640815972352

Morphometric study of changes in GAD 65 expressing beta cell mass in the islets of langerhans in pregnant mice

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