Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells

Tetracera indica Merr.(Family: DILLENIACEAE) is a large, woody, rain forest climber of Malaysia which is commonly known as Mempelas paya or sand paper plant. Two flavonoids MHQ-1 (wogonin) and MHQ-2 (norwogonin) were isolated from the stems ethanol extract of T. indica. Moreover, MHQ-1 and MHQ-2...

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Main Authors:	Ahmed, Qamar Uddin, Hasan, Mohd Mehmudul, Sarian, Murni Nazira, Mat So'ad, Siti Zaiton, Bakhtiar, M. Taher, Latip, Jalifah
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/65520/ http://irep.iium.edu.my/65520/1/rcstss2016_abstracts_ST_final_secured.pdf

id	iium-65520
recordtype	eprints
spelling	iium-655202018-09-18T04:30:10Z http://irep.iium.edu.my/65520/ Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah RS403 Materia Medica-Pharmaceutical Chemistry Tetracera indica Merr.(Family: DILLENIACEAE) is a large, woody, rain forest climber of Malaysia which is commonly known as Mempelas paya or sand paper plant. Two flavonoids MHQ-1 (wogonin) and MHQ-2 (norwogonin) were isolated from the stems ethanol extract of T. indica. Moreover, MHQ-1 and MHQ-2 including rosiglitazone as positive control were subjected to fluorescence glucose uptake test by 2-NBDG (fluorescent glucose analog) on mature adipocytes. Wogonin was found to show more cytotoxicity (IC50 <25 µg/mL) in comparison to norwogonin (no IC50 upto100 µg/mL) in MTT viability assay on 3T3-L1 preadipocyte cells. Structure activity relationships (SAR) study of both the flavonoids revealed that the methoxy (O-Me) substitution (wogonin) at position 8 of ring A of flavone skeleton is essential for inducing cytotoxicity on 3T3-L1 Pre-adipocytes. However, hydroxyl (OH) group at the same position (norwogonin) is safe to the cells (percent viability is above 80% for all the doses evaluated). Additionally, both the compounds were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails. The adipogenic cocktails were modified by the compounds and rosiglitazone in the presence or absence of insulin. The results of adipogenesis showed that MHQ-1 and MHQ-2 induced adipogenesis like insulin and enhanced adipogenesis like rosiglitazone significantly in three different concentrations. Furthermore, both the compound stimulated the 2-NBDG uptake on mature adipocytes significantly. Results further confirm the traditional use of T. indica in the management of diabetes in Malaysia. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/65520/1/rcstss2016_abstracts_ST_final_secured.pdf Ahmed, Qamar Uddin and Hasan, Mohd Mehmudul and Sarian, Murni Nazira and Mat So'ad, Siti Zaiton and Bakhtiar, M. Taher and Latip, Jalifah (2016) Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells. In: Regional Conference On Science, Technology & Social Sciences 2016 (RCSTSS), 4th - 6th December 2016, Cameron Highlands, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	RS403 Materia Medica-Pharmaceutical Chemistry Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells
description	Tetracera indica Merr.(Family: DILLENIACEAE) is a large, woody, rain forest climber of Malaysia which is commonly known as Mempelas paya or sand paper plant. Two flavonoids MHQ-1 (wogonin) and MHQ-2 (norwogonin) were isolated from the stems ethanol extract of T. indica. Moreover, MHQ-1 and MHQ-2 including rosiglitazone as positive control were subjected to fluorescence glucose uptake test by 2-NBDG (fluorescent glucose analog) on mature adipocytes. Wogonin was found to show more cytotoxicity (IC50 <25 µg/mL) in comparison to norwogonin (no IC50 upto100 µg/mL) in MTT viability assay on 3T3-L1 preadipocyte cells. Structure activity relationships (SAR) study of both the flavonoids revealed that the methoxy (O-Me) substitution (wogonin) at position 8 of ring A of flavone skeleton is essential for inducing cytotoxicity on 3T3-L1 Pre-adipocytes. However, hydroxyl (OH) group at the same position (norwogonin) is safe to the cells (percent viability is above 80% for all the doses evaluated). Additionally, both the compounds were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails. The adipogenic cocktails were modified by the compounds and rosiglitazone in the presence or absence of insulin. The results of adipogenesis showed that MHQ-1 and MHQ-2 induced adipogenesis like insulin and enhanced adipogenesis like rosiglitazone significantly in three different concentrations. Furthermore, both the compound stimulated the 2-NBDG uptake on mature adipocytes significantly. Results further confirm the traditional use of T. indica in the management of diabetes in Malaysia.
format	Conference or Workshop Item
author	Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah
author_facet	Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah
author_sort	Ahmed, Qamar Uddin
title	Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells
title_short	Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells
title_full	Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells
title_fullStr	Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells
title_full_unstemmed	Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells
title_sort	flavonoids from tetracera indica merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3t3-l1 adipocyte cells
publishDate	2016
url	http://irep.iium.edu.my/65520/ http://irep.iium.edu.my/65520/1/rcstss2016_abstracts_ST_final_secured.pdf
first_indexed	2023-09-18T21:32:58Z
last_indexed	2023-09-18T21:32:58Z
_version_	1777412633531514880

Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells

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