Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression

Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression

IQGAPs genes play critical role in either induction or suppression of cancer and its progression, however the relationship between Ras genes and these genes are still unclear. In this study, we tried to understand the mechanistic action of IQGAPs genes and its correlation with Ras genes in mouse hep...

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Main Authors: Zoheir, Khairy MA, Abd-Rabou, Ahmed A, Harisa, Gamaleldin I., Ashour, Abdelkader Elbadawy Abbas, Ahmad, Sheikh Fayaz, Attia, Sabry M, Bakheet, Saleh A, Abdel-Hamied, Hala E., Abd-Allah, Adel R. A., Kumar, Ashok
Format: Article
Language:English
Published: e-Century Publishing Corporation 2015
Subjects:
RM300 Drugs and their action
Online Access:http://irep.iium.edu.my/65376/
http://irep.iium.edu.my/65376/
http://irep.iium.edu.my/65376/
http://irep.iium.edu.my/65376/7/65376%20Gene%20expression%20of%20IQGAPs%20and%20Ras%20families.pdf
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recordtype eprints
spelling iium-653762018-09-14T09:21:40Z http://irep.iium.edu.my/65376/ Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression Zoheir, Khairy MA Abd-Rabou, Ahmed A Harisa, Gamaleldin I. Ashour, Abdelkader Elbadawy Abbas Ahmad, Sheikh Fayaz Attia, Sabry M Bakheet, Saleh A Abdel-Hamied, Hala E. Abd-Allah, Adel R. A. Kumar, Ashok RM300 Drugs and their action IQGAPs genes play critical role in either induction or suppression of cancer and its progression, however the relationship between Ras genes and these genes are still unclear. In this study, we tried to understand the mechanistic action of IQGAPs genes and its correlation with Ras genes in mouse hepatic cancer model. The genetic expressions of IQGAP1, IQGAP2, IQGAP3, Hras, Kras, Nras, Mras, Caspase3, and BAX were followed in both hepatocellular carcinoma and normal liver cells of Balbc mice. Genotoxic agent diethylnitrosamine (DEN)-induced hepatic cancer model was induced in male mice and recorded the occurrence of hepatocellular carcinoma by morphological and histological changes in the liver. It was observed that mRNA expressions of IQGAP1, Hras, Kras, Nras, Mras, Caspase3, and BAX genes were highly elevated in hepatocellular carcinoma cells when compared with normal liver cells, additionally their expressions increased by concentrating the dose of DEN. While, the expressions of IQGAP2 and IQGAP3 were significantly decreased in hepatocellular carcinoma cells when compared with normal liver cells, as well as their expressions decreased more with increasing the dose of DEN. It was concluded from this study that IQGAP1 has a strong signaling relationship with Ras genes in induction of cancer and it is considered as a key gene for induction or suppression of the hepatocellular carcinoma. e-Century Publishing Corporation 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65376/7/65376%20Gene%20expression%20of%20IQGAPs%20and%20Ras%20families.pdf Zoheir, Khairy MA and Abd-Rabou, Ahmed A and Harisa, Gamaleldin I. and Ashour, Abdelkader Elbadawy Abbas and Ahmad, Sheikh Fayaz and Attia, Sabry M and Bakheet, Saleh A and Abdel-Hamied, Hala E. and Abd-Allah, Adel R. A. and Kumar, Ashok (2015) Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression. International Journal of Clinical and Experimental Pathology, 8 (8). pp. 8821-8831. ISSN 1936-2625 http://www.ijcep.com/files/ijcep0010544.pdf 1936-2625/IJCEP0010544
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RM300 Drugs and their action
spellingShingle RM300 Drugs and their action
Zoheir, Khairy MA
Abd-Rabou, Ahmed A
Harisa, Gamaleldin I.
Ashour, Abdelkader Elbadawy Abbas
Ahmad, Sheikh Fayaz
Attia, Sabry M
Bakheet, Saleh A
Abdel-Hamied, Hala E.
Abd-Allah, Adel R. A.
Kumar, Ashok
Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression
description IQGAPs genes play critical role in either induction or suppression of cancer and its progression, however the relationship between Ras genes and these genes are still unclear. In this study, we tried to understand the mechanistic action of IQGAPs genes and its correlation with Ras genes in mouse hepatic cancer model. The genetic expressions of IQGAP1, IQGAP2, IQGAP3, Hras, Kras, Nras, Mras, Caspase3, and BAX were followed in both hepatocellular carcinoma and normal liver cells of Balbc mice. Genotoxic agent diethylnitrosamine (DEN)-induced hepatic cancer model was induced in male mice and recorded the occurrence of hepatocellular carcinoma by morphological and histological changes in the liver. It was observed that mRNA expressions of IQGAP1, Hras, Kras, Nras, Mras, Caspase3, and BAX genes were highly elevated in hepatocellular carcinoma cells when compared with normal liver cells, additionally their expressions increased by concentrating the dose of DEN. While, the expressions of IQGAP2 and IQGAP3 were significantly decreased in hepatocellular carcinoma cells when compared with normal liver cells, as well as their expressions decreased more with increasing the dose of DEN. It was concluded from this study that IQGAP1 has a strong signaling relationship with Ras genes in induction of cancer and it is considered as a key gene for induction or suppression of the hepatocellular carcinoma.
format Article
author Zoheir, Khairy MA
Abd-Rabou, Ahmed A
Harisa, Gamaleldin I.
Ashour, Abdelkader Elbadawy Abbas
Ahmad, Sheikh Fayaz
Attia, Sabry M
Bakheet, Saleh A
Abdel-Hamied, Hala E.
Abd-Allah, Adel R. A.
Kumar, Ashok
author_facet Zoheir, Khairy MA
Abd-Rabou, Ahmed A
Harisa, Gamaleldin I.
Ashour, Abdelkader Elbadawy Abbas
Ahmad, Sheikh Fayaz
Attia, Sabry M
Bakheet, Saleh A
Abdel-Hamied, Hala E.
Abd-Allah, Adel R. A.
Kumar, Ashok
author_sort Zoheir, Khairy MA
title Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression
title_short Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression
title_full Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression
title_fullStr Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression
title_full_unstemmed Gene expression of IQGAPs and Ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression
title_sort gene expression of iqgaps and ras families in an experimental mouse model for hepatocellular carcinoma: a mechanistic study of cancer progression
publisher e-Century Publishing Corporation
publishDate 2015
url http://irep.iium.edu.my/65376/
http://irep.iium.edu.my/65376/
http://irep.iium.edu.my/65376/
http://irep.iium.edu.my/65376/7/65376%20Gene%20expression%20of%20IQGAPs%20and%20Ras%20families.pdf
first_indexed 2023-09-18T21:32:46Z
last_indexed 2023-09-18T21:32:46Z
_version_ 1777412620701138944

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