Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
2016
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/1/The%20International%20Medical%20Journal%20Malaysia%20%282016%29%20Vol%2015%20Supplementary%20issue%20No1.pdf |
| Summary: | Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming
because biofilms induce antibiotic resistance to an order of three or more in magnitude
greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious
organism that causes the hallmark of chronic infections including hospital acquired infections
that leads to high morbidity and mortality. One of the reasons for the occurrence of
resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms
against a series of metal-antibiotics, an alternative to the conventional antibiotics, was
investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was
synthesized to give metal-gentamicin complexes. The metal contents of all the compounds
were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing
of the gentamicin-antibiotic complexes against the biofilms was conducted using broth
microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all
the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II)
complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show
that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25
to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these
metal-antibiotics are also proposed. |
|---|