Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms
Introduction: Dental plaque is a structurally and functionally organized biofilm. Modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. Inherent resistance of biofilm bacteria to conventional antibiot...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/65146/ http://irep.iium.edu.my/65146/ http://irep.iium.edu.my/65146/1/The%20International%20Medical%20Journal%20Malaysia%20%282017%29%20Vol16%20Supplementary%20issue%20no2.pdf |
| Summary: | Introduction: Dental plaque is a structurally and functionally organized biofilm. Modern molecular
biological techniques have identified about 1000 different bacterial species in the dental biofilm,
twice as many as can be cultured. Inherent resistance of biofilm bacteria to conventional
antibiotics is alarming. It induces antibiotic resistance to an order of three or more in magnitude
greater than those displayed by planktonic bacteria. Staphylococcus aureus is the most dominant
bacterial species isolated from the saliva and dental plaques. One of the reasons for its
pathogenicity is its ability to form biofilms. In this study, the resistance of S. aureus biofilms
against a eries of metal-antibiotics, an alternative to the conventional antibiotics, was
investigated. Materials and Methods: A series of metal-antibiotic complexes derived from
gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the
compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility
testing of the gentamicin-antibiotic complexes against several strains of S. aureus biofilms was
conducted using broth microdilution assay. Results: The results showed that S. aureus is
susceptible against Co(II) and Fe(II) gentamicin complexes; all were tested at 0.25 to 1 mmol
concentrations. Conclusion(s): Co(II) and Fe(II)-gentamicin complexes demonstrated antimicrobial
activity. |
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